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Colloidal gold immunocolorimetry kit for detecting lipoprotein (Lpa) and preparation method of kit

A colloidal gold and kit technology, applied in the field of in vitro diagnostic reagents, can solve the problems of narrow species distribution and limited basic research progress, and achieve the effects of high specificity, good repeatability and high accuracy

Inactive Publication Date: 2016-08-24
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Lpa has a narrow species distribution and is only found in humans, Old World monkeys, and European hedgehogs, thus limiting progress in basic research

Method used

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  • Colloidal gold immunocolorimetry kit for detecting lipoprotein (Lpa) and preparation method of kit
  • Colloidal gold immunocolorimetry kit for detecting lipoprotein (Lpa) and preparation method of kit
  • Colloidal gold immunocolorimetry kit for detecting lipoprotein (Lpa) and preparation method of kit

Examples

Experimental program
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Embodiment 1

[0029] Preparation of Lpa calibrator

[0030] Dissolve the recombinant human Lpa protein (commercially available) in the calibrator diluent (bovine serum albumin 0.5%, sodium azide 0.1%, PBS buffer pH 8.0) to prepare calibrator with different concentration gradients, and use Jiuqiang Lpa latex to immunize The colorimetric kit detects and prepares calibrator, detects each concentration gradient 3 times, takes the average value of the test results, and the fixed value is 0mg / L (calibration substance diluent, no Lpa protein added), 112.5mg / L, 225mg / L , 350mg / L, 700mg / L, 1400mg / L.

[0031] Preparation of Reaction Buffer Reagent (Reagent 1)

[0032] Take 80mL of 50mM PBS pH8.0 buffer and add PEG6000-200005g, polysucrose-400 1g, bovine serum albumin 0.5g, sodium azide 0.1g, and finally use the corresponding buffer to dilute to 100mL, and use 0.22um micro Filter through a pore filter to make a reaction buffer reagent (reagent 1), including PEG6000-200005%, polysucrose-4001%, bovine...

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Abstract

The invention provides a colloidal gold immunocolorimetry kit for detecting lipoprotein (Lpa) and a preparation method of the kit and belongs to the field of in-vitro diagnostic reagents. The kit is used for judging whether the situation of individual acute kidney injury occurs or not. According to the method, colloidal gold particles are adopted as a carrier, a lipoprotein (Lpa for short) antibody and the colloidal gold particles are coupled, Lpa protein in a sample makes contact with the coupled gold particles in a corresponding detection device, an antigen-antibody-gold compound is formed, the light absorbance of the gold particles under detection wavelength is changed, the change amount of the light absorbance is positively correlated with the concentration of Lpa protein, and then the purpose of detecting Lpa protein in the sample is achieved. The kit has high analysis sensitivity.

Description

field of invention [0001] The invention belongs to the field of in vitro diagnostic reagents, in particular to a colloidal gold immunocolorimetric assay kit for detecting lipoprotein a (Lpa) technical background [0002] Lipoprotein a (lipoprotein a, Lpa) is one of the important members of lipoprotein macromolecules, and it is also a hot spot in lipoprotein research. [0003] In 1963, Berg, a Norwegian geneticist in Northern Europe, immunized rabbits with the human low-density lipoprotein (LDL) of the subjects to produce an antiserum against a certain antigenic component in the LDL component. Berg made this new discovery The antigen component named Lpa. In 1972, Dalhen et al. first discovered that there was a pre-β-lipoprotein in the plasma lipoprotein profile of many patients with coronary heart disease, and confirmed that this zone was Lpa. In 1975, Dahlen et al. considered that Lpa was a risk factor for AS. In 1987, McLean et al. found that apo(a) had high homology wit...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 郭成志王志新
Owner CHEMCLIN DIAGNOSTICS CO LTD