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Method for improving fertility of tetraploid cucumis melo

A tetraploid and melon technology, which is applied in the agricultural field, can solve the problems of low reproduction coefficient, prolonged breeding period, affecting the breeding efficiency of tetraploid melon, etc., and achieves the effects of improving the reproducibility rate and shortening the breeding period.

Active Publication Date: 2016-08-31
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tetraploid melons have low nirvana, which means that their reproductive coefficient is low, which is the biggest obstacle to the utilization of tetraploid melons and seriously affects the breeding efficiency of tetraploid melons.
[0004] At present, it is mainly through continuous selfing of tetraploid melons to improve their mellowness. Although the method is feasible, the breeding cycle is long, and it often takes more than 4 generations of selfing (generally takes 2 years) to improve the mellowness of tetraploid melons significantly. Prolonged breeding cycle and high breeding costs

Method used

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  • Method for improving fertility of tetraploid cucumis melo
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  • Method for improving fertility of tetraploid cucumis melo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] From February to June, 2015, the method provided by the invention was used in the main production area of ​​melons in Luwo Town, Wuming County, Nanning City, Guangxi to carry out cultivation comparative experiments.

[0060] Among them, the rootstock is the diploid thin-skinned melon JM, and the scion is the autotetraploid melon JM4n induced by diploid thin-skinned melon JM through colchicine induction. A total of 1,200 plants were planted. At the same time, a control was set up. The control was the tetraploid seedlings of melons, and the vertical cultivation method was adopted in the protected area of ​​the greenhouse. The area of ​​the test field was 1 mu, and a total of 1200 plants were planted.

[0061] In this embodiment, a grafting stick is directly inserted 8 mm obliquely from the growth point of the removed rootstock, slightly penetrating the rootstock epidermis. Afterwards, obliquely cut at 2 mm below the base of the tetraploid muskmelon scion cotyledon with a...

Embodiment 2

[0066] From February to June 2015, the present invention was used to carry out comparative cultivation experiments in the main production area of ​​melons in Luwo Town, Wuming County, Nanning City, Guangxi.

[0067] What is different from Example 1 is that in this test, the grafting stick is directly inserted obliquely from the growth point of the removed rootstock by 10 mm, and correspondingly, it is obliquely cut with a blade at the base of the tetraploid melon scion cotyledon 2 mm, and the length of the cut surface is 9mm.

[0068] The tetraploid melon fruit setting rate obtained by the method of the present invention, the average number of seeds per fruit, the average number of normal seeds per fruit and the yield of fruit are all improved compared with the control, and the yield of fruit is 49.75%, which is 168.05% higher than the 18.56% of the contrast. %, the hardness of tetraploid melon seeds was greatly improved (see Table 2 for details).

[0069] Table 2 Comparison ...

Embodiment 3

[0073] From February to June 2015, the present invention was used to carry out comparative cultivation experiments in the main production area of ​​melons in Luwo Town, Wuming County, Nanning City, Guangxi.

[0074] The difference from Example 1 is that the rootstock is diploid muskmelon KM, and the scion is tetraploid muskmelon JM4n.

[0075] The fruit setting rate of tetraploid melon obtained by the method of the present invention, the average number of seeds per fruit, the average number of normal seeds per fruit and the seed yield are all improved compared with the control, and the seed yield is 46.41%, which is 19.15% higher than that of the contrast. 142.35%, the hardness of tetraploid melon seeds was greatly improved (see Table 3 for details).

[0076] Table 3 Comparison of fruit setting rate, average single fruit seed number, normal seed number, sputum number and seed yield of tetraploid melon

[0077]

[0078] Obviously, in conjunction with Table 1 and Table 3, it...

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Abstract

The invention discloses a method for improving the fertility of tetraploid cucumis melo, and relates to the technical field of agriculture. The time-wasting seed breeding process in the prior art can be avoided, and the seed breeding period of tetraploid cucumis melo can be effectively shortened. The method for improving the fertility of tetraploid cucumis melo includes the steps that diploid cucumis melo is dibbled to serve as stock; after a few days, tetraploid cucumis melo is seeded to serve as scions; tetraploid cucumis melo scion seedlings are grafted to diploid cucumis melo stock seedlings; grafted seedlings are cultivated; harvesting and seed collecting are carried out after tetraploid cucumis melo is mature, and the fertility of tetraploid cucumis melo is checked. The method is applied to seed breeding of tetraploid cucumis melo.

Description

technical field [0001] The invention relates to the field of agriculture, in particular to a method for improving the tenderness of tetraploid melons. Background technique [0002] melon( Cucumis melo ) belongs to the genus Cucurbitaceae Melon, including two types of thin-skinned melon (commonly known as pear melon) and thick-skinned melon (cantaloupe, cantaloupe). It is one of the top ten fruits in the world and has a long history of cultivation in my country. Melon fruit is sweet and rich in carbohydrates, protein, minerals and other vitamins and other nutrients, and is very popular among the public. Common melon is a diploid plant, and the number of chromosomes in its somatic cells is 2n=24. Through artificial induction, the chromosomes of diploid melon plant cells can be doubled to become tetraploid (4n=48). The fruit of this tetraploid melon increases, and the content of vitamin C, sugar, protein and carotenoids The material is improved, the resistance to disease and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/06
CPCA01G2/30
Inventor 黄金艳龙明华温东强李文信
Owner GUANGXI UNIV