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A kind of method of sassafras isolated plant regeneration

An isolated and plant-based technology, applied in the field of plant tissue culture, can solve the problems of unreported sassafras tissue culture technology, and achieve the effects of easy large-scale promotion and use, improved economic benefits, and high survival rate of transplanting

Active Publication Date: 2017-12-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the tissue culture technique of sassafras has not been reported

Method used

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  • A kind of method of sassafras isolated plant regeneration
  • A kind of method of sassafras isolated plant regeneration
  • A kind of method of sassafras isolated plant regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1 A kind of high-efficiency detached plant regeneration method of sassafras camphor adult excellent single plant

[0066] (1) Collection and disinfection of explants: select the fine single plant of sassafras camphora that is straight and perfect in dry type, beautiful in tree posture, fast in growth rate, rich in camphor and camphor oil, free from diseases and insect pests, and more than 15 years old as the female parent. The selected excellent trees were subjected to base circumcision to promote base sprouting. Before collecting explants, alternately spray sprouting shoots with carbendazim and chlorothalonil for 5 times every 7 days; when the weather was fine in October at around 10:00 in the morning, cut the trunk The semi-lignified shoots at the base, after removing the leaves and moisturizing treatment, take them back to the laboratory, wash them with pure water and store them in a refrigerator at 4°C for later use; then cut the branches into 4cm long ste...

Embodiment 2

[0072]Embodiment 2 A kind of high-efficiency detached plant regeneration method of sassafras camphor adult excellent single plant

[0073] (1) Collection and disinfection of explants: select the fine single plant of sassafras camphora that is straight and perfect in dry type, beautiful in tree posture, fast in growth rate, rich in camphor and camphor oil, free from diseases and insect pests, and more than 15 years old as the female parent. The selected excellent trees were subjected to base circumcision to promote base sprouting. Before collecting explants, alternately spray sprouting shoots with carbendazim and chlorothalonil for 5 times every 8 days; when the weather was fine in September at around 10:00 in the morning, cut the trunk The semi-lignified shoots at the base, after removing the leaves, take them back to the laboratory after moisturizing treatment, wash them with pure water and store them in a 4°C refrigerator for later use; then cut the branches into 3 cm long, 2...

Embodiment 3

[0079] Embodiment 3 A kind of high-efficiency detached plant regeneration method of sassafras camphor adult excellent single plant

[0080] (1) Collection and disinfection of explants: select the fine single plant of sassafras camphora that is straight and perfect in dry type, beautiful in tree posture, fast in growth rate, rich in camphor and camphor oil, free from diseases and insect pests, and more than 15 years old as the female parent. The selected excellent trees were subjected to base circumcision to promote base sprouting. Before collecting explants, alternately spray sprouting shoots with carbendazim and chlorothalonil for 4 times every 10 days; when the weather was fine in November at around 10 a.m., cut the trunk The semi-lignified shoots at the base, after removing the leaves, take them back to the laboratory after moisturizing treatment, wash them with pure water and store them in a 4°C refrigerator for later use; then cut the branches into 2cm long stems with a le...

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Abstract

The invention belongs to the technical field of plant tissue culture, and concretely relates to a Cinnamomum porrectum (Roxb.) Kosterm. in vitro plant regeneration method. The Cinnamomum porrectum (Roxb.) Kosterm. in vitro plant regeneration method comprises the steps of (1) explant collection, (2) axillalry bud induction, (3) multiplication culture, (4) rooting culture, (5) seedling hardening and transplanting, and (6) transplanted seedling management. The high-efficiency in vitro plant regeneration and rapid propagation method is established through selecting a basic medium and screening the kind, the concentration and the proportion of hormones by adopting excellent adult single plants with the advantages of fast growth speed, straight trunk, excellent wood properties, high camphor and camphor wood oil content and strong stress resistance as a female parent and a basal sprout as an explant in order to realize vigorous and fast elongation growth of multiplied buds, high multiplication rate, high rooting rate, vigorous rooted seedlings and high transplanting survival rate.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for regeneration of isolated sassafras plants. Background technique [0002] Sassafras (Cinnamomum porrectum (Roxb.) Kosterm.) belongs to Lauraceae (Lauraceae) genus (Cinnamomum), the adult tree height can reach 20m, and the diameter at breast height is more than 40cm. Sassafras is produced in Guangzhou, Guangxi, Fujian, Jiangxi, Hunan, Guizhou, Sichuan, Yunnan and other places in China. Sassafras is also distributed in Pakistan and India via Malaysia to Indonesia. [0003] Sassafras is an evergreen tree with beautiful tree posture, obvious, straight and smooth trunk, and fast growth. Its average annual growth of diameter at breast height is 50% larger than that of camphor, and its volume is 100% larger. It is an excellent fast-growing tree species. Sassafras wood has a straight texture, uniform and fine structure, slightly heavy and tough, eas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 邓小梅白红娟奚如春鲁好君赵帅陈晓阳
Owner SOUTH CHINA AGRI UNIV