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Application of Gracilariopsis lemaneiformis polysaccharide as antitumor chemotherapy drug synergist and antitumor drug

A technology of asparagus polysaccharides and anti-tumor drugs, which can be applied in the direction of anti-tumor drugs, drug combinations, heavy metal active ingredients, etc., can solve the problems of drug applications that have not been reported, and achieve the effect of enriching drug sources and inhibiting tumor proliferation and activity

Inactive Publication Date: 2016-08-31
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Asparagus polysaccharide is a natural compound, which is obviously different from the commonly used cytotoxic antineoplastic drugs, and the application of asparagus polysaccharide as a chemotherapeutic synergist has not been reported yet

Method used

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  • Application of Gracilariopsis lemaneiformis polysaccharide as antitumor chemotherapy drug synergist and antitumor drug
  • Application of Gracilariopsis lemaneiformis polysaccharide as antitumor chemotherapy drug synergist and antitumor drug
  • Application of Gracilariopsis lemaneiformis polysaccharide as antitumor chemotherapy drug synergist and antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Antiproliferative Effects of Asparagus Polysaccharides on Three Kinds of Tumor Cells

[0029] Experimental materials: asparagus polysaccharide is a polysaccharide extracted and purified from the red algae asparagus. Asparagus is a 981 variety, collected from Wenzhou, Zhejiang. Human lung cancer cell line A549, gastric cancer cell line MKN45 and cervical cancer cell line Hela were purchased from the Cell Bank of the Chinese Academy of Sciences and preserved and cultured in our laboratory.

[0030] Experimental method: adding ddH to purified and dried asparagus polysaccharide 2 O was made into a 1mg / mL stock solution, sterilized by filtration, aliquoted, and stored at -20°C for later use. Human lung cancer cell line A549, gastric cancer cell line MKN45 and cervical cancer cell line Hela are all adherent cells. 6 Seed in fresh 1640 medium (containing 89v / v% RPMI1640 medium, 10v / v% fetal bovine serum FBS, 1v / v% 1U / mL penicillin-streptomycin) at an inoculation density o...

Embodiment 2

[0035] Pharmacodynamic study on anti-tumor synergistic effect of asparagus polysaccharides on paclitaxel

[0036] Experimental material: asparagus polysaccharide is the finished product of asparagus polysaccharide obtained by adopting the extraction and separation method provided by the present invention, the same as in Example 1. Paclitaxel injection (trade name: Paclitaxel, English name: Paclitaxel Injection) Beijing Concord Pharmaceutical Factory (approval number: Guoyao Zhunzi H10980069), the specification is 5mL: 30mg. Human lung cancer cell line A549, gastric cancer cell line MKN45 and cervical cancer cell line Hela were routinely cultured in RPMI1640 medium containing 10% fetal bovine serum (FBS).

[0037] Experimental method: In this example, asparagus polysaccharide was used in combination with paclitaxel, a first-line drug used to treat various solid tumors, to act on human lung cancer cell line A549, gastric cancer cell line MKN45, and cervical cancer cell line Hela...

Embodiment 3

[0043] Pharmacodynamic study on anti-tumor synergistic effect of asparagus polysaccharides on fluorouracil

[0044] Experimental materials: except that paclitaxel was replaced by fluorouracil injection, other materials were the same as in Example 2. The fluorouracil injection (English name: Fluorouracil Injection) used was purchased from Yabao Pharmaceutical Group Co., Ltd. (approval number: Guoyao Zhunzi H20057995 ), the specification is 10mL:0.25g.

[0045] Experimental content: except that the added drug to be tested is fluorouracil or fluorouracil and Asparagus polysaccharide, the others are the same as in Example 2. The experimental results are shown in Table 2 and image 3 Shown:

[0046] Table 2 Tumor cell growth inhibition rate (%) ( n=6)

[0047]

[0048] Note: Compared with the fluorouracil group, ** means P<0.01, * means P<0.05

[0049] The above results show that when the chemotherapeutic drug fluorouracil is used alone, 2.5g / mL fluorouracil has a certain ...

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Abstract

The invention provides application of Gracilariopsis lemaneiformis polysaccharide and an antitumor drug containing Gracilariopsis lemaneiformis polysaccharide. The application refers to application of the Gracilariopsis lemaneiformis polysaccharide as a synergist in antitumor drug preparation. The antitumor drug contains a Gracilariopsis lemaneiformis polysaccharide synergist, antitumor active components and pharmaceutically acceptable excipients. The invention for the first time finds that combined use of seaweed Gracilariopsis lemaneiformis polysaccharide with chemotherapy drugs paclitaxel, fluorouracil, methotrexate, cisplatin and the like can increase the treatment effect of chemotherapy drugs on tumors. The combined use of Gracilariopsis lemaneiformis polysaccharide with chemotherapy drugs can achieve the effect of inhibiting tumor proliferation and activity on a plurality of tumor cells with different tissue origins, including lung cancer, gastric cancer, cervical cancer and the like, the synergistic effect of Gracilariopsis lemaneiformis polysaccharide on chemotherapy drugs is dose-dependent, and in Gracilariopsis lemaneiformis polysaccharide in-vitro cell experiments, the working concentration does not show toxicity, with the characteristics of rich medicine sources, safety and no toxicity, the Gracilariopsis lemaneiformis polysaccharide can be developed into antitumor drugs or preventive health care products used for antitumor chemotherapy drug synergists, and has clinical application prospect.

Description

technical field [0001] The invention relates to the application of a marine red alga asparagus polysaccharide and an antitumor drug containing the asparagus polysaccharide, belonging to the field of biomedicine, especially the technical field of synergizing the effect of marine traditional Chinese medicine on tumor chemotherapeutic drugs. Background technique [0002] The development of low-toxicity and high-efficiency anti-tumor drugs by using abundant seaweed is a hot field in the research of new natural active drugs. Common tumor chemotherapy drugs have good therapeutic effects but have relatively large side effects. Traditional Chinese medicine assisting Western medicine in the treatment of tumors has positive significance in enhancing the efficacy of chemotherapy drugs, improving the body's immunity, prolonging survival, reducing recurrence and metastasis rates, and reducing side effects. Liu Ji et al. reported that curcumin has an enhanced effect on the anti-gastric c...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61K36/04A61P35/00A61K31/337A61K31/513A61K33/24A61K31/519
CPCA61K31/715A61K31/337A61K31/513A61K31/519A61K33/24A61K36/04
Inventor 康亚妮赵小东邵志峰张伟伟
Owner SHANGHAI JIAO TONG UNIV
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