Novel method for preparing double-violet injection

Abstract

The invention provides a novel method for preparing a double-violet injection. The double-violet injection is prepared from dry whole plants of dandelion and Chinese violet as medicines, wherein the weight ratio of the dandelion to the Chinese violet is 1 to 1. The method comprises the following steps: (a) cutting the dandelion and the Chinese violet into segments, crushing the segments and adding the product to an extraction tank; (b) adding purified water to the extraction tank, heating the extraction tank to 80-85 DEG C, soaking and cooling the product 45-55 DEG C, adjusting the pH value into acidity, adding plant disintegrating composite enzyme, stirring at a constant speed, standing and decomposing the product; (c) decocting and filtering the mixture in the extraction tank to obtain final filtrate; (d) adding a flocculant to the final filtrate and carrying out centrifugal flocculation to obtain supernate; and (e) carrying out vacuum nanofiltration and concentration on the supernate by a nanofiltration membrane, wherein the end-point concentration of concentration is 0.95-1.05g / ml. According to the double-violet injection prepared by the method, effective ingredients in the dandelion and the Chinese violet can be effectively extracted and collected.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals and relates to Shuangding injection, in particular to a new method for preparing Shuangding injection. Background technique [0002] Shuangding Injection is a pure traditional Chinese medicine injection mainly for clinical mastitis in dairy cows, which is included in the third volume P118 of "National Standards for Raising Local Standards for Veterinary Drugs". The main ingredients are dandelion (also known as dandelion) and violet. [0003] Dandelion whole herb is used as medicine, nature and flavor: bitter sweet, cold; enters the liver, stomach meridian. Function: heat-clearing and toxic substances removing, inducing diuresis and resolving stagnation. Indications: acute mastitis, lymphadenitis, furunculosis, acute conjunctivitis, cold and fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infection, etc. The whole herb of dandelion contains dandeli...

AI Technical Summary

Problems solved by technology

[0005] Because most of the active ingredients of Chinese medicinal materials exist in plant cells, and the cell wall components of plant cells are mainly fibers, pectin, etc., the active ingredients of Chinese medicinal materials are blocked by the cell wall and released incompletely, which affects the full extraction and utilization of active ingredients of medicinal materials.

And because the molecular weights of active ingredients such as inulin, pectin, flavonoids, and organic acids contained in Shuangding Injection are all less than 1000, the traditional alcohol precipitation method will usually cause the loss of these small molecular weight substances, affecting The effect of medicine