Method for preparing silk-fibroin-based water-absorbing material by grafting vinyl monomer

A water-absorbing material and vinyl monomer technology, applied in the direction of fermentation, etc., can solve the problems of the number of phenolic oxygen radicals, low reactivity or accessibility, difficult and efficient graft copolymerization, affecting the preparation of silk fibroin-based water-absorbing materials, etc. The effect of avoiding silk fibroin denaturation, safe treatment process, and mild enzyme treatment conditions

Active Publication Date: 2016-09-07
无锡市奥菲超细织物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Silk fibroin contains a certain number of tyrosine residues, and HRP can also catalyze the phenolic hydroxyl radicals of tyrosine to generate phenolic oxygen free radicals; on the other hand, although the phenolic oxygen free radicals produced by oxidation can react with vinyl monomers (such as AM, AA) graft copolymerization occurs, but due to the relatively low number, reactivity or accessibility of phenolic oxygen radicals on silk, it is difficult to form efficient graft copolymerization with AM and AA, which affects the silk fibroin-based water-absorbing materials. preparation of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The degummed mulberry silk is used to prepare the silk fibroin-based water-absorbing material by the method mentioned in the present invention, and the specific process steps are as follows:

[0025] (1) Preparation of silk fibroin solution: add the degummed silk into an ethanol solution of lithium bromide, stir at 70°C until dissolved, and then dialyze with deionized water for 40 hours to obtain a silk fibroin solution;

[0026] (2) Graft vinyl-containing carboxylic acid on the surface of silk fibroin: add EDC / NHS activation system and acrylic acid to the silk fibroin solution, among which silk fibroin 10g / L, EDC 2g / L, NHS 2g / L, acrylic acid 0.5g / L, treatment temperature 25°C, pH 6, time 6 hours;

[0027] (3) Graft copolymerization of enzymatic silk fibroin with AM and AA: in the silk fibroin solution in step (2), add horseradish peroxidase, acetylacetone, AM and AA, and add H 2 o 2 , to promote graft copolymerization of silk fibroin with AM and AA; horseradish pero...

Embodiment 2

[0034] The degummed mulberry silk is used to prepare the silk fibroin-based water-absorbing material by the method mentioned in the present invention, and the specific process steps are as follows:

[0035] (1) Preparation of silk fibroin solution: add the degummed silk to an ethanol solution of calcium chloride, stir at 70°C until dissolved, and then dialyze with deionized water for 30 hours to obtain a silk fibroin solution;

[0036](2) Vinyl-containing carboxylic acid grafted on the surface of silk fibroin: Add EDC / NHS activation system and crotonic acid to silk fibroin solution, in which silk fibroin 25g / L, EDC 3g / L, NHS 3g / L, butyric acid Dienoic acid 1.5g / L, treatment temperature 30°C, pH 6, time 12 hours;

[0037] (3) Graft copolymerization of enzymatic silk fibroin with AM and AA: in the silk fibroin solution in step (2), add horseradish peroxidase, acetylacetone, AM and AA, and add H 2 o 2 , to promote graft copolymerization of silk fibroin with AM and AA; horseradi...

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Abstract

The invention discloses a method for preparing a silk-fibroin-based water-absorbing material by grafting a vinyl monomer. Vinyl-containing carboxylic acid is grafted on the silk fibroin surface by the aid of EDC/NHS (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide), acrylamide (AM) and acrylic acid (AA) monomers are added, and a horseradish peroxidase/oxydol/acetylacetonate system is utilized to catalyze the silk fibroin-vinyl monomer graft copolymerization, thereby preparing the silk-fibroin-based water-absorbing material by a bioenzyme process. The method comprises the following steps: (1) preparing a silk fibroin solution; (2) grafting vinyl onto the silk fibroin surface by an EDC/NHS process; (3) catalyzing the silk fibroin-AM-AA graft copolymerization by using the horseradish peroxidase; and (4) forming the silk-fibroin-based water-absorbing material. Compared with the traditional process, the method has the advantages of high graft copolymerization efficiency and mild enzyme treatment conditions. The silk-fibroin-based composite water-absorbing material has the advantages of favorable water absorptivity, favorable water retention property and favorable biodegradability.

Description

technical field [0001] The present invention relates to a method for preparing silk fibroin-based water-absorbing materials by grafting vinyl monomers, in particular to a method of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC ) / N-hydroxysuccinimide (NHS), graft vinyl-containing carboxylic acid on the surface of silk fibroin, then add acrylamide (AM) and acrylic acid (AA) monomers, catalyzed by horseradish peroxidase The invention relates to graft copolymerization of silk fibroin and monomers to prepare silk fibroin-based water-absorbing materials, belonging to the field of textile biotechnology. Background technique [0002] Silk fibroin is the main component of silk, which is composed of 18 kinds of amino acids, most of which are low-polarity tyrosine and alanine chain segments, and the rest are mainly composed of polar amino acid residues. Silk fibroin has good biocompatibility and environmental friendliness, and the recycled materials processed with silk fibroin h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F289/00C08F220/56C08F220/06C08J5/18C12P13/02
CPCC08F289/00C08J5/18C08J2351/00C12P13/02C08F220/56
Inventor 王平周步光袁久刚王强范雪荣
Owner 无锡市奥菲超细织物有限公司
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