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Method for preparing fibroin-base biomimetic mineralization material by grafting vinyl monomers

A technology of biomimetic mineralization and vinyl single, which is applied in the field of textile biology, can solve the problems affecting the preparation of silk fibroin-based biomimetic mineralization materials, the number of carboxyl groups on the surface of silk fibroin increases, and the difficulty of high-efficiency graft copolymerization, etc., to increase the calcium ion network Combined ability, enhanced effect, effect on process safety

Active Publication Date: 2017-08-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, although HRP can catalyze the generation of phenolic oxygen radicals from tyrosine residues in silk fibroin and initiate graft copolymerization with acrylic acid monomers, due to the small number of tyrosine residues on silk fibroin or the enzymatic reaction The medium accessibility is low, and it is difficult to efficiently graft and copolymerize with acrylic acid monomers, so that the number of carboxyl groups on the surface of silk fibroin increases less after the reaction, which affects the preparation of silk fibroin-based biomimetic mineralization materials

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0022] The silk fibroin-based biomimetic mineralization material is prepared by the method mentioned in the present invention, and the specific process steps are as follows:

[0023] (1) Preparation of silk fibroin solution: add the degummed silk into the ethanol solution of lithium bromide or calcium chloride, stir at 70°C until dissolved, and then dialyze with deionized water for 24 hours to obtain 20g / L silk fibroin protein solution;

[0024] (2) Grafting carboxylic acid containing vinyl and phenolic hydroxyl groups by EDC / NHS method: add EDC, NHS and caffeic acid to the silk fibroin solution in step (1) for treatment, wherein EDC 3g / L, NHS 2g / L, coffee Acid 2g / L, temperature 25°C, pH 5.5, treatment time 12 hours;

[0025] (3) Horseradish peroxidase catalyzes silk fibroin and acrylic acid graft copolymerization: in the silk fibroin solution of step (2), add horseradish peroxidase, acetylacetone and acrylic acid, add hydrogen peroxide under nitrogen protection, promote Gra...

Embodiment 2

[0033] The silk fibroin-based biomimetic mineralization material is prepared by the method mentioned in the present invention, and the specific process steps are as follows:

[0034](1) Preparation of silk fibroin solution: Add the degummed silk into the ethanol solution of lithium bromide or calcium chloride, stir at 70°C until dissolved, and then dialyze with deionized water for 36 hours to obtain 25g / L silk fibroin protein solution;

[0035] (2) EDC / NHS method grafting contains vinyl and phenolic hydroxyl carboxylic acid: add EDC, NHS and ferulic acid in the silk fibroin solution of step (1) and process, wherein EDC 5g / L, NHS 4g / L, Ferulic acid 4g / L, temperature 25°C, pH 5.75, treatment time 18 hours;

[0036] (3) Horseradish peroxidase catalyzes silk fibroin and acrylic acid graft copolymerization: in the silk fibroin solution of step (2), add horseradish peroxidase, acetylacetone and acrylic acid, add hydrogen peroxide under nitrogen protection, promote Silk fibroin and...

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Abstract

The invention discloses a method for preparing a fibroin-base biomimetic mineralization material by grafting vinyl monomers. According to the method, carbodiimide (EDC) and N-hydroxy succinimide (NHS) are grafted with carboxylic acid containing ethylene and phenolic hydroxy groups on fibroin macromolecules, and horseradish peroxidase is utilized for catalyzing graft copolymerization of modified fibroin and acrylic acid monomers so as to obtain the fibroin-base biomimetic mineralization material. The method specifically comprises the following steps: (1) preparing a fibroin protein solution; (2) grafting carboxylic acid containing ethylene and phenolic hydroxy groups by virtue of an EDC / NHS method; (3) catalyzing graft copolymerization of the fibroin and the acrylic acid monomers; (4) forming a fibroin film protein; and (5) preparing the fibroin-base biomimetic mineralization material. Compared with the traditional methods, such as a chemical method or an ultraviolet irradiation, for triggering graft copolymerization of the fibroin and the acrylic acid monomers, the method has the advantages that the graft copolymerization efficiency of the fibroin and the acrylic acid monomers is high, the processing conditions are mild, and the prepared fibroin-base biomimetic mineralization material has excellent performance.

Description

technical field [0001] The invention relates to a method for preparing silk fibroin-based biomimetic mineralization materials by grafting vinyl monomers. Oxidase catalyzes graft copolymerization of modified silk fibroin and acrylic acid monomers to prepare silk fibroin-based biomimetic mineralization materials, which belongs to the field of textile biotechnology. Background technique [0002] Silk fibroin is the main component of silk, which is composed of 18 kinds of amino acids, most of which are low-polarity tyrosine and alanine chain segments, and the rest are mainly composed of polar amino acid residues. Silk fibroin has good biocompatibility and environmental friendliness, and the recycled materials processed with silk fibroin have the characteristics of non-toxic, non-irritating, and excellent hygroscopicity. After the silk fiber is treated with calcium salt or lithium salt and its alcohol solution, the macromolecules of silk fibroin are dissolved and partially hydro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/00C08J5/18C08F289/00C08F220/06
CPCC08F289/00C08J5/18C08J7/00C08J2351/00C08F220/06
Inventor 王平邓超崔莉徐进范雪荣
Owner JIANGNAN UNIV
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