Extraction and composition analysis method for organic phosphorus in lake aquatic plants

A technology for aquatic plants and extraction methods, which is applied in the fields of nuclear magnetic resonance analysis, color/spectral characteristic measurement, etc. It can solve the problems of low extraction rate and inability to simultaneously measure the content of organic phosphorus and inorganic phosphorus, etc., and achieve a good peak effect , the effect of high extraction rate

Inactive Publication Date: 2016-09-07
CHINESE RES ACAD OF ENVIRONMENTAL SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

What this invention discloses is the extraction method of deposit, and its shortcoming is that the extraction rate is not high
CN104614331A discloses a method for simultaneously extracting and analyzing the forms of organic phosphorus and inorganic phosphorus in water sediments to solve the problem that the existing phosphorus form extraction method cannot simultaneously measure the content of organic phosphorus and inorganic phosphorus in water sediments
H

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extraction and composition analysis method for organic phosphorus in lake aquatic plants
  • Extraction and composition analysis method for organic phosphorus in lake aquatic plants
  • Extraction and composition analysis method for organic phosphorus in lake aquatic plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] A method for extracting organic phosphorus in lake aquatic plants, comprising the following steps:

[0031] (1) Sample collection: collect the whole plants of aquatic plants, put them into sealed bags for refrigerated storage, freeze-dry the collected samples, grind them thoroughly, pass through a 2mm sieve to obtain aquatic plant powder samples, and store them in refrigerated storage at 4°C for later use;

[0032] (2) Determination of the basic composition of phosphorus: total phosphorus TP, inorganic phosphorus P i and P o the determination of

[0033] Accurately weigh two samples of 0.5g aquatic plant powder, each with 3 parallel samples, one is calcined in a muffle furnace at 450°C for 3h, after cooling down to room temperature, transfer to a centrifuge tube, add 20mL3.5mol / L HCl and shake for 16h , after centrifugation, the supernatant was filtered with a 0.45 μm filter membrane to obtain an extract, digested with 5% potassium persulfate at 121°C for 30 minutes, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an extraction method for organic phosphorus in lake aquatic plants. The extraction method comprises the steps of sample collection, phosphoric basic composition determination, organic phosphorus (Po) extraction, sample testing pretreatment and liquid nuclear magnetism (31P-NMR) analysis and calculation. According to the method, the optimal Po extraction effect is obtained by adopting NaOH with the concentration of 0.5 mol/L and an EDTA extraction agent with the concentration of 25 mmol/L on the condition that the solid-to-liquid ratio of aquatic plant powder to the extraction agent is 1:60; in the 31P-NMR analysis and test process, the delay time (D1) is set as 5 s, the scanning and analyzing time is set as 15 h, and a good spectrogram can be obtained. The aquatic plant Po extraction method has the advantages that the average extraction rate of total phosphorus (TP) and Po is 91.4% or above and 88.1% or above respectively, the specific variety and content of Po in a sample can be accurately detected, and the composition structure characteristics of Po in the lake aquatic plants can be accurately reflected; a scientific basis is provided for research on the biogeochemical characteristics of Po in the lake aquatic plants and contribution of Po in algal bloom.

Description

technical field [0001] The invention relates to a method for extracting and analyzing the composition of organic phosphorus, in particular to a method for extracting and analyzing the composition of organic phosphorus in aquatic plants in lakes. Background technique [0002] Phosphorus is one of the key limiting nutrients in aquatic ecosystems and an important factor affecting lake eutrophication. Exogenous input and endogenous circulation are the main factors leading to the substantial increase of phosphorus in lake water. In recent years, the input of exogenous phosphorus, including domestic sewage, industrial and agricultural production, and soil runoff, has been controlled to a certain extent, and the release of endogenous phosphorus in lakes has become a key factor causing further deterioration of lake water quality. Sediment release is one of the important sources of endogenous phosphorus pollution in lakes, but the products of death and decomposition of aquatic plant...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N24/08G01N21/33
CPCG01N24/08G01N21/33
Inventor 冯伟莹吴丰昌朱元荣张琛
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap