A sesquiterpene compound, its preparation method and its application in the preparation of anti-tobacco mosaic virus medicine
A technology of sesquiterpenoids and compounds, applied in the field of sesquiterpenoids, can solve the problems of unidentified components, etc., and achieve the effects of good anti-tobacco mosaic virus activity, simple extraction method, and easy industrial production
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] The tobacco samples were from Yuxi, Yunnan, and the variety was Yuxi K326. Sampling 2.0 kg of tobacco was pulverized and extracted 5 times with 95% methanol for 24 hours each time. The extracts were combined, filtered, and concentrated under reduced pressure to form an extract to obtain 105 g of extract. After dissolving the extract with 2.0 times the amount of pure methanol by weight, mix the sample with 120g of 100-mesh silica gel, pack 0.6kg of 160-mesh silica gel into a column for silica gel column chromatography, and use a volume ratio of 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 8: The chloroform-acetone eluted part of 2 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 48% methanol as mobile phase, Zorbax SB-C18 (21.2×250mm, 5 μm) preparative column as stationary phase, and the flow rate was 20ml / m...
Embodiment 2
[0036] The tobacco sample comes from Dali, Yunnan, and the variety is Yunyan 200. The tobacco sample is 3.5kg and chopped, and extracted 4 times with 95% ethanol, each extraction is 48h, the extracts are combined, filtered, and concentrated under reduced pressure to obtain an extract. 250g. The extract is dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 250 g of 80 mesh thick silica gel, and 1.2 kg of 200 mesh silica gel is packed for silica gel column chromatography, and the volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 8: The chloroform-acetone eluted part of 2 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 48% methanol as mobile phase, Zorbax SB-C18 (21.2×250mm, 5 μm) preparative column as stationary phase, and the flow rate was 20ml / min, the detection wavelength...
Embodiment 3
[0038] The tobacco sample comes from Kunming, Yunnan, and the variety is Honghua Dajinyuan. The tobacco sample is 5kg and pulverized, and extracted 3 times with 75% acetone by ultrasound, each extraction is 72h, the extracts are combined, filtered, and concentrated under reduced pressure to form an extract. Obtain extractum 380g. The extract is dissolved in pure methanol with a weight ratio of 1.6 times, and then mixed with 400 g of 90 mesh thick silica gel, and 2.4 kg of 180 mesh silica gel is packed into a column for silica gel column chromatography, and the volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 8: The chloroform-acetone eluted part of 2 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 48% methanol as mobile phase, Zorbax SB-C18 (21.2×250mm, 5 μm) preparative column as stationary phase, and ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| degree of unsaturation | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More