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Method for measuring carbon, hydrogen and nitrogen stable isotope ratios of nicotine in electronic tobacco smoke solution

An e-cigarette liquid and isotope ratio technology is applied in the determination of hydrogen and nitrogen stable isotope ratios, and in the field of nicotine carbon in e-cigarette liquid, achieving the effects of low cost and easy operation.

Inactive Publication Date: 2016-09-21
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no method for determining the stable isotope ratios of nicotine carbon, hydrogen, and nitrogen in e-cigarette liquid at home and abroad.

Method used

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  • Method for measuring carbon, hydrogen and nitrogen stable isotope ratios of nicotine in electronic tobacco smoke solution
  • Method for measuring carbon, hydrogen and nitrogen stable isotope ratios of nicotine in electronic tobacco smoke solution
  • Method for measuring carbon, hydrogen and nitrogen stable isotope ratios of nicotine in electronic tobacco smoke solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Instruments and reagents

[0028] Isotope mass spectrometer (AS1310 autosampler, Trace GC Ultra gas chromatograph, GC IsoLink combustion and thermal conversion device (combustion device is used to determine carbon and nitrogen in nicotine, thermal conversion device is used to determine hydrogen in nicotine) , Delta VAdvantage stable isotope ratio mass spectrometer. Thermo Fisher, USA); AE163 electronic balance (sensitivity: 0.0001g, Mettler, Switzerland); high-purity helium (99.999%, carrier gas); nicotine reference material comes from Indiana, USA University Isotope Laboratory, #2 (δ 13 C =7.72±0.02‰, δ 15 N=-5.94±0.15‰), #3 (δ 13 C=-30.05±0.02‰, δ 15 N=-33.62±0.18‰), #4 (δ 13 C=-2.06±0.02‰, δ 15 N=-15.49±0.13‰), #5 (δ 13 C=-29.63±0.01‰, δ 15 N=--6.03±0.04‰, δD=-161.30±1.7‰); methanol (chromatographically pure, TEDIA, USA).

[0029] 2. Sample pretreatment

[0030] The e-cigarette liquid was diluted with methanol, and the extract was mixed evenly under the c...

Embodiment 2

[0046] According to the measurement method described in Example 1, select an electronic cigarette liquid B, and measure the δ of nicotine in it 13 C, δD and δ 15 N were -31.13, -246.88 and -7.30, respectively.

Embodiment 3

[0048] According to the measurement method described in Example 1, select an electronic cigarette liquid B, and measure the δ of nicotine in it 13 C, δD and δ 15 N are -32.30, -248.203 and -5.38, respectively.

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Abstract

The invention provides a method for measuring the carbon, hydrogen and nitrogen stable isotope ratios of nicotine in an electronic tobacco smoke solution. The method is characterized by comprising the following steps of: performing liquid sampling after diluting the electronic tobacco smoke solution with an organic solvent; separating nicotine from other impurities of 1,2-propanediol and propanetriol by use of a gas chromatograph provided with a capillary chromatography column; analyzing the carbon stable isotope ratio value delta13C, hydrogen stable isotope ratio deltaD and nitrogen stable isotope ratio delta15N of nicotine in the electronic tobacco smoke solution by use of gas chromatography-isotope mass spectrograph (GC-IRMS); and calibrating and calculating the delta12C, deltaD and delta15N of nicotine in the electronic tobacco smoke solution according to delta12C, deltaD and delta15N measured values of a nicotine isotopic standard substance. By use of a GC-IRMS technology, measurement of the carbon, hydrogen and nitrogen stable isotope ratios of nicotine in the electronic tobacco smoke solution is realized for the first time at home and abroad, and a technical method is provided for judging nicotine sources in the electronic tobacco smoke solution.

Description

technical field [0001] The invention belongs to the technical field of stable isotope analysis, and in particular relates to a method for measuring the ratios of nicotine carbon, hydrogen and nitrogen stable isotopes in electronic cigarette liquid. Background technique [0002] The topic of smoking and health has attracted more and more people's attention. In recent years, people have developed new tobacco products represented by electronic cigarettes and snus. The emergence of these new tobacco products has gradually changed people's tobacco consumption structure [1, 2] . Most e-cigarette liquids contain nicotine, but the source of nicotine in e-cigarette liquids is still unclear, which is not conducive to the quality and safety control of e-cigarette products. [0003] There are many methods to determine the source of nicotine, such as stable isotope ratio method, optical rotation method, characteristic component analysis method, etc. Among them, stable isotope ratio met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/025
Inventor 韩书磊陈欢马潇付亚宁刘彤侯宏卫胡清源
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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