Method for extracting wheat oligopeptide from wheat

Abstract

The invention relates to a method for extracting wheat oligopeptide from wheat. The method comprises the following specific steps: (1) thoroughly rinsing harvested fresh wheat kernels with water, draining surface moisture, and then placing the drained wheat kernels in a fresh keeping warehouse for storage for 2-6 hours; (2) taking out the wheat kernels from the fresh keeping warehouse, sending the wheat kernels taken from the fresh keeping warehouse into a crusher for coarse crushing, performing screening with a 30-40-mesh sieve so as to obtain coarse wheat kernel powder, sending the coarse wheat kernel powder into microwave treatment equipment, and performing interval microwave treatment; (3) sending the coarse wheat kernel after microwave treatment into a grinding machine, performing continuous grinding for 4 hours, performing screening with a 120-180-mesh sieve plate, then putting screened wheat kernel powder into a stainless steel container, and adding warm water for diluting the screened wheat kernel powder so as to obtain wheat kernel paste; (4) heating the wheat kernel paste to 45 DEG C, adding papain to the paste, performing uniform stirring, maintaining the temperature at 40-60 DEG C, and performing leaching for 1-4 hours so as to obtain an extracting solution for standby application; (5) taking the extracting solution, and performing vacuum sucking filtration with a circulating water type multi-purpose vacuum pump so as to obtain a peptide solution after sucking filtration; and (6) performing vacuum spray drying on the raw material peptide solution, and collecting drying substances namely the wheat oligopeptide.

Description

Technical field

[0001] The invention relates to the technical field of bioengineering, in particular to a method for extracting wheat oligopeptides from wheat. Background technique

[0002] Wheat oligopeptide is an essential active substance that exists in the human body itself, and is an important component and nutrient substance of the human body. It is widely distributed throughout the human body. Scientists have made rapid progress in the research of wheat oligopeptide peptides in the past ten years. Studies have confirmed that, in addition to the nutritional effects of general protein, peptides also have a very important and irreplaceable regulatory effect on the human body. This effect involves almost all physiological activities of the human body, such as nerves, digestion, absorption, and metabolism. , Circulation, growth, reproduction, endocrine, etc.

[0003] Wheat oligopeptides are made from wheat gluten powder through processes such as pulping, protease hydrolysis, se...