Preparation method of lentinan

A technology for lentinan and lentinan, which is applied in the directions of metabolic diseases, anti-tumor drugs, antidote, etc., can solve the problems of low extraction rate of lentinan, hindering the development of lentinan industry, and low activity, and achieves improved extraction rate and total antioxidant capacity. The effect of improved ability and simple preparation method

Inactive Publication Date: 2016-09-28
高枫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the extraction rate of lentinan is low and the activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Take shiitake mushrooms, remove impurities and wash, drain and chop;

[0025] 2) Put the shiitake mushrooms obtained in step 1) into a high-pressure tank, add deionized water three times the weight of the shiitake mushrooms, pressurize to 26MPa, perform high-pressure treatment for 5 minutes, and release the pressure to obtain a paste-like slurry;

[0026] 3) Add deionized water 10 times the weight of the original shiitake mushrooms to the paste slurry, and perform ultrasonic extraction at a frequency of 110 kHz and a temperature of 65° C. for 20 minutes to obtain an ultrasonic extract;

[0027] 4) Ultrafilter the ultrasonic extraction solution with a membrane with a pore size of 10nm, concentrate the filtrate to a relative density of 1.1, then add an ethanol solution with a volume concentration 11 times the weight of the original shiitake mushrooms, and carry out alcohol precipitation and alcohol analysis for 20h, 4000r Centrifuge for 20 min at 1 / min, discard the sup...

Embodiment 2

[0029] 1) Take shiitake mushrooms, remove impurities and wash, drain and chop;

[0030] 2) Put the shiitake mushrooms obtained in step 1) into a high-pressure tank, add deionized water 4 times the weight of shiitake mushrooms, pressurize to 24MPa, perform high-pressure treatment for 7 minutes, and release the pressure to obtain a paste-like slurry;

[0031] 3) Add deionized water 9 times the weight of the original shiitake mushrooms to the paste slurry, and perform ultrasonic extraction at a frequency of 130 kHz and a temperature of 60° C. for 25 minutes to obtain an ultrasonic extract;

[0032] 4) Ultrafilter the ultrasonic extraction solution with a membrane with a pore size of 10nm, concentrate the filtrate to a relative density of 1.3, then add an ethanol solution with a volume concentration 10 times the weight of the original shiitake mushrooms, and carry out alcohol precipitation, alcohol analysis for 19h, 4200r / min centrifuged for 18min, the supernatant was discarded, ...

Embodiment 3

[0034] 1) Take shiitake mushrooms, remove impurities and wash, drain and chop;

[0035] 2) Put the shiitake mushrooms obtained in step 1) into a high-pressure tank, add deionized water 5 times the weight of shiitake mushrooms, pressurize to 22MPa, perform high-pressure treatment for 9 minutes, and release the pressure to obtain a paste-like slurry;

[0036] 3) Add deionized water 8 times the weight of the original shiitake mushrooms to the paste slurry, and perform ultrasonic extraction at a frequency of 150 kHz and a temperature of 55° C. for 30 minutes to obtain an ultrasonic extract;

[0037] 4) Ultrafilter the ultrasonic extraction solution with a membrane with a pore size of 15nm, concentrate the filtrate to a relative density of 1.2, then add an ethanol solution with a volume concentration 9 times the weight of the original shiitake mushrooms, and carry out alcohol precipitation and alcohol analysis for 18h, 4400r Centrifuge for 15 min at 100°C, discard the supernatant, ...

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Abstract

The invention belongs to the technical field of biological extraction, and particularly relates to a preparation method of lentinan. The method comprises the following steps: taking pasania fungus, purifying, washing, draining off and cutting; secondly, performing high pressure treatment; thirdly, performing ultrasonic extraction; and fourthly, performing ultrafiltration, concentrating, alcohol precipitation and centrifugation, discarding supernatant, and performing vacuum freeze drying, so as to obtain the lentinan. The lentinan prepared through the method is high in extraction ratio, and various efficacies, such as anticancer efficacy, antioxidant efficacy and blood fat reducing efficacy, of the lentinan can be improved.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, in particular to a preparation method of lentinan. Background technique [0002] my country has become a major producer of edible fungi. By 2013, the production of edible fungi produced in my country accounted for more than 70% of the world's total output. The production of edible fungi has become the three major rural industries with equal emphasis on planting and breeding. It is an important way for farmers in mountainous or poor areas of our country to get rid of poverty and become rich. However, there are serious phenomena such as large consumption of raw materials, low economic efficiency, and inverted output ratio in the production of edible fungi in my country. Research on a medium formula to improve the yield and quality of edible fungi has become a new demand in the edible fungus market. [0003] Shiitake mushrooms belong to the phylum Fungi, Agaricaceae, Pleurotaceae, an...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00A61P39/06A61P3/06
CPCC08B37/0003C08B37/0024
Inventor 高枫
Owner 高枫
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