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New diagnostic assay using particles with magnetic properties

A technology of magnetic particles, particles, used in measurement devices, other chemical processes, separation methods, etc.

Inactive Publication Date: 2018-04-03
LAB ON A BEAD AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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One problem is the control of particle movement

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  • New diagnostic assay using particles with magnetic properties
  • New diagnostic assay using particles with magnetic properties
  • New diagnostic assay using particles with magnetic properties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0128] Example 1. Preparation of epoxide-activated agarose particles

[0129] Sepharose 4B (GE Healthcare Life Sciences) was washed with distilled water on a glass filter and blotted dry. 3 g of xerogel particles were suspended in 2.4 ml of 1M sodium hydroxide solution, and 0.45 ml of epichlorohydrin was added dropwise with stirring at room temperature. The temperature was raised to 60°C and maintained for 2 hours. The epoxide-activated agarose gel was washed neutral with distilled water on a glass filter and resuspended in distilled water at a final 50% gel concentration. This product constitutes an example of a porous particle as defined herein, see for example, figure 1 and figure 2 Particles in Pp.

Embodiment 2

[0130] Example 2. Production of agarose particles modified with magnetic particles comprising magnetic particles of one size.

[0131] Amino (NH 2 ) functionality of commercially available magnetic microparticles as magnetic particles. These particles are examples of magnetic particles as defined herein, see, for example, figure 1 and figure 2 Magnetic particles in Mp.

[0132] Make the magnetic particles as follows M-NH 2 (2 μm and 10 μm) were covalently attached to porous epoxy-activated agarose particles, respectively. will be from Micromod Partikelteknologie GmbH -M-NH 2 (250μL, 7×10 8 particles / ml) were washed twice with 1000 μL PBS (15 mM phosphate, 150 mM NaCl, pH 7.4) and resuspended in 1000 μL PBS. Epoxide-activated agarose particles, i.e. 1 ml of settled gel, were resuspended in 10 ml of 0.01 M NaOH solution, added to the magnetic particle suspension, and reacted at room temperature under vortexing, for 2 μm particles 12 hours, and 30 minutes for 10 μm...

Embodiment 3

[0134] Example 3. Production of agarose particles modified with magnetic particles comprising magnetic particles of two sizes

[0135] Make the magnetic particles as follows M-NH 2 (5 μm and 10 μm) covalently attached to porous epoxy-activated agarose particles:

[0136] First, 10 μm from Micromod Partikelteknologie GmbH -M-NH 2 (250μL, 7×10 8 particles / ml) were washed twice with 1000 μL PBS (15 mM phosphate, 150 mM NaCl, pH 7.4) and resuspended in 1000 μL PBS. Epoxide-activated agarose particles, that is, 1 ml of the settled gel, were resuspended in 10 ml of 0.01 M NaOH solution, added to the 10 μm magnetic particle suspension, and reacted for 30 minutes at room temperature under vortex shaking.

[0137] Second, 5 μm from Micromod Partikelteknologie GmbH -M-NH 2 (250μL, 7×10 8 particles / ml) were washed twice with 1000 μL PBS (15 mM phosphate, 150 mM NaCl, pH 7.4) and resuspended in 1000 μL PBS. Then 5 μm magnetic particles were added to the agarose 10 μm magneti...

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PUM

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Abstract

A particle comprising non-magnetic porous particles comprising at least one polymer and having an outer surface and an inner surface bounded by the pores. Nonmagnetic porous particles have functional groups on their inner and outer surfaces. Magnetic particles, which also have functional groups bound to their surfaces, are covalently bonded to non-magnetic porous particles by reacting the functional groups of the two particles and forming a covalent bond between them. The smallest average diameter of the magnetic particles is larger than the average diameter of the pores of the porous particles. In this way, the magnetic particles do not obstruct the pores of the non-magnetic porous particles. The present application also relates to novel systems and assays for the analysis of molecules and cells using magnetic particles. Magnetic particles located on non-magnetic particles form a signature pattern, fingerprint or barcode.

Description

technical field [0001] The present description generally relates to novel systems for the analysis of molecules and cells comprising clusters in which non-magnetic particles are supplemented with magnetic particles to form characteristic patterns or bar codes. Such particles and methods for their production are also disclosed. Background technique [0002] Techniques for analyzing biomolecules and cells in bodily fluids are critical in many technical fields such as, but not limited to, medicine, biopharmaceuticals, and biotechnology. Traditional immunological techniques such as enzyme-linked immunosorbent assay (ELISA) are still predominant. However, as the number of analyzes performed worldwide continues to increase, the need for faster and more efficient analytical methods becomes critical. Systems that rely on multiplex analysis represent one solution to a more efficient analytical system. Several commercial technologies using multiplex detection are now available. Ho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/281B01D15/38C08G81/00
CPCB01D15/3885B01D15/3804B01J20/285B01J20/286B01J20/3204B01J20/321B01J20/3212B01J20/3242B01J20/3265B01J20/28009B01J20/28016B01J20/24B01J20/3217C08B37/0039G01N33/5434G01N33/548
Inventor 斯文·奥斯卡尔森克里斯托弗·埃里克松彼得·斯韦德林德
Owner LAB ON A BEAD AB
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