Ecological poria cocos wild imitating tending technology and application
A wild-like, ecological technology, applied in application, fertilizer mixture, horticulture, etc., can solve problems such as ecological environment damage, achieve high content of active ingredients, protect pine forest resources, better treatment and health care effects
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Embodiment 1
[0025] A kind of ecological Poria cocos imitation wild raising technology, concrete operation is as follows:
[0026]1. Production of strains: (1) Production of Poria cocos mother species: use Poria cocos samples with higher content of active ingredient Poria cocos polysaccharides (4.186%) in the wild Poria cocos samples collected in the Dabie Mountains to isolate the strains on PDA slant medium, as First-class mother species. The specific production method of the mother seed is: medium formula: 150g of potatoes (peeled), 10g of sucrose, 10g of agar, 1g of urea, and 1000mL of water. The above formula was made into a test tube slant medium according to a conventional method. And under sterile conditions, the tissue separation method was used. Put a piece of white Poria meat in the sclerotium of Poria cocos into the medium slope. Move it into a room at 30°C and cultivate it for 7 days until the mycelia cover the slope, and then it becomes the first-class mother species. (2) ...
Embodiment 2
[0035] A kind of ecological Poria cocos imitation wild raising technology, concrete operation is as follows:
[0036] 1. Production of strains: (1) Production of Poria cocos mother species: use Poria cocos samples with higher content of active ingredient Poria cocos polysaccharides (4.186%) in the wild Poria cocos samples collected in the Dabie Mountains to isolate the strains on PDA slant medium, as First-class mother species. The specific production method of the mother seed is: medium formula: 180g of potatoes (peeled), 15g of sucrose, 12g of agar, 2g of urea, and 1000mL of water. The above formula was made into a test tube slant medium according to a conventional method. And under sterile conditions, the tissue separation method was used. Put a piece of white Poria meat in the sclerotium of Poria cocos into the medium slope. Move it into a room at 30°C and cultivate it for 7 days until the mycelia cover the slope, and then it becomes the first-class mother species. (2)...
Embodiment 3
[0045] A kind of ecological Poria cocos imitation wild raising technology, concrete operation is as follows:
[0046] 1. Production of strains: (1) Production of Poria cocos mother species: use Poria cocos samples with higher content of active ingredient Poria cocos polysaccharides (4.186%) in the wild Poria cocos samples collected in the Dabie Mountains to isolate the strains on PDA slant medium, as First-class mother species. The specific production method of the mother species is: medium formula: 200g of potatoes (peeled), 20g of sucrose, 18g of agar, 2g of urea, and 1000mL of water. The above formula was made into a test tube slant medium according to a conventional method. And under sterile conditions, the tissue separation method was used. Put a piece of white Poria meat in the sclerotium of Poria cocos into the medium slope. Move it into a room at 30°C and cultivate it for 7 days until the mycelia cover the slope, and then it becomes the first-class mother species. ...
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