Application of fungus hydrophobin HGFI to trypsin immobilization

A fungal hydrophobin, trypsin technology, applied in the direction of immobilization on/in an organic carrier, can solve the problem of inability to use fungal hydrophobin, improve hydrophobicity and biocompatibility, increase the number of uses, improve applied effect

Active Publication Date: 2016-10-12
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved by the present invention is that fungal hydrophobin cannot be used in the prior art when trypsin is immobilized.

Method used

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  • Application of fungus hydrophobin HGFI to trypsin immobilization
  • Application of fungus hydrophobin HGFI to trypsin immobilization
  • Application of fungus hydrophobin HGFI to trypsin immobilization

Examples

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Embodiment 1

[0028] (1) prepare the trypsin PBS solution that concentration is 1mg / mL;

[0029] (2) preparation concentration is the fungal hydrophobin HGFI aqueous solution of 100 μ g / mL;

[0030] (3) Take 100 μL of the fungal hydrophobin HGFI aqueous solution and drop it on the surface of polystyrene, let it stand overnight at room temperature, and modify it to obtain polystyrene modified with fungal hydrophobin HGFI;

[0031] (4) Draw 100uL of trypsin PBS solution and drop it on the polystyrene surface modified by fungal hydrophobin HGFI, and let it stand at room temperature for 30 minutes;

[0032] (5) Absorb the residual liquid and wash the polystyrene surface with PBS solution.

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Abstract

The invention relates to application of fungus hydrophobin HGFI to trypsin immobilization. Firstly, specific protein in the fungus hydrophobin is used for achieving immobilization of trypsin, wherein the efficient self-assembly film formation and immobilization capabilities of the hydrophobin HGFI are fully utilized, the surface of a polystryrene material is modified through the fungus hydrophobin HGFI via a static adsorption method, the trypsin is immobilized to the surface, modified by the HGFI, of the polystryrene through the static acting force, the hydrophobicity and the biocompatibility of the polystyrene are improved through the biological source performance and the high surface activity of the HGFI, finally, the catalytic activity of the trypsin on the surface of the hydrophobic carrier is improved, the service life of the trypsin is prolonged, and the frequency of using of the trypsin is increased. The application of the trypsin to scientific researches and industries is improved.

Description

technical field [0001] The invention relates to the application of fungal hydrophobin HGFI in trypsin solidification, and belongs to the field of biotechnology. Background technique [0002] Trypsin is a proteolytic enzyme extracted from animal pancreas. It has been widely used in scientific research and industry because it can specifically cut the carboxyl terminus of lysine and arginine residues in peptide chains. in production. In view of the instability of trypsin, in order to improve its stability and use efficiency, it is necessary to immobilize trypsin. [0003] At present, the strategies for trypsin immobilization mainly include embedding method, covalent cross-linking method and physical adsorption method. Compared with the other two methods, the physical adsorption method has the advantages of simple operation, saving time and effort, and easy to control the amount of enzyme; at the same time, this method can better retain the natural structure and catalytic acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08
CPCC12N11/08
Inventor 牛宝龙李文凤李炳章王慧芳郭睿劼马森源魏丽乔
Owner TAIYUAN UNIV OF TECH
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