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A method for rapid asexual reproduction of Changshan Huyou

A technology of asexual reproduction and pomelo, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low survival rate, difficulty in adapting to the production and development of Changshan pomelo, poor fruit taste, etc., and achieve high efficiency Effect

Inactive Publication Date: 2017-11-28
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fruit trees of Changshan pomelo after multi-generation propagation are not easy to preserve some of the excellent traits of the female parent, and the resulting fruit tastes bad. Some fruit farmers often use cuttings for propagation, but the propagation speed of cuttings is slow and the survival rate is not high. , it is difficult to adapt to the needs of the production and development of Changshan Huyou

Method used

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  • A method for rapid asexual reproduction of Changshan Huyou
  • A method for rapid asexual reproduction of Changshan Huyou
  • A method for rapid asexual reproduction of Changshan Huyou

Examples

Experimental program
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Effect test

Embodiment 1

[0018] (1) Selection and processing of explants:

[0019] The young stems, young leaves and young fruits with young buds of Huyou Changshan were selected as explants. Specifically, Changshan Huyou was planted in an experimental greenhouse, and watered at the right time to keep its growth in good condition. During the germination period of Huyou, the young stems and young leaves with buds of Huyou are obtained by clipping. If there is no new shoot, carry out topping treatment to Huyou, new shoots will grow after 2 weeks, and young stems and young leaves suitable for the experiment can be obtained after 3-4 weeks. Young fruit about 3 months after flowering, young fruit with a diameter of 2-3cm, put the young fruit at 4°C for 3 days, take it out, wrap it with plastic wrap and put it at 37°C for 60 minutes, wash it with detergent and soak After 5 minutes, rinse with distilled water to obtain the pretreated young fruit, and peel off the immature embryo with a size of 1-3mm on the...

Embodiment 2

[0044] The immature embryos isolated from the pretreated young fruit in Example 1 were inoculated into cluster bud induction medium (6-BA 0.5mg / L+BRs 0.1mg / L+AgNO 3 1mg / L MS modified medium), cultivated for about 30 days at 22±2°C, under the condition of 1000-1200lux light, to obtain clustered buds, which were cut into individuals with terminal buds, and then transferred to rooting medium (0.1- 0.2mg / L IAA MS modified medium or 0.1-0.2mg / L IAA+0.5mg / L 6-BA MS modified medium, see Table 5), at a temperature of 22±2°C and a light of 1000-1200lux Cultivate for about 30 days, and finally form a complete plant. On average, each cluster bud can form more than 6 plants after cutting. Roots can be grown on MS modified medium + 0.1-0.2 mg / L IAA, and MS modified medium + 0.1-0.2 mg / LIAA + 0.5 mg / L 6-BA. In about 30 days, the root length can reach 2-3 cm, further forming a complete plant. Therefore, after the above-mentioned cultivation, an immature embryo can obtain clustered buds w...

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Abstract

The invention discloses a method of quick vegetative propagation of citrus changshanensis. The method includes: inoculating a disinfected explant into a tufted bud inducing culture medium, culturing at 20-24 DEG C and under illumination of 1000-1200 lux, and transferring one time every other 25-45 days for culture for 2-3 times to obtain a tufted bud; cutting the tufted bud into an individual with a top bud, inoculating the individual into a rooting culture medium, and culturing at 24-26 DEG C and under 1000-1200 lux for 30-60 days to form a complete plant. By utilizing the method, at least 6 plants can be obtained by culturing a young embryo for about 3 months, so that efficiency is quite high, a lot of citrus changshanensis seedlings can be propagated and obtained within a short time, and the method can be applied in production practice.

Description

(1) Technical field [0001] The invention relates to a method for rapid asexual reproduction of Changshan Huyou. (2) Background technology [0002] Changshan Huyou is a new species of Citrus in China. Its fruit is plump and bright yellow in appearance; the fruit is golden yellow and full of juice; it has a strong fragrance, moderately sweet and sour taste, and slightly sweet; it is popular among the masses and reflects good economic benefits. In recent years, the plantation area has stepped up the construction of Changshan pomelo orchards, and the seed area has continued to expand; moreover, more attention has been paid to the yield and fruit quality of pomelo. The fruit trees of Changshan pomelo after multi-generation propagation are not easy to preserve some of the excellent traits of the female parent, and the resulting fruit tastes bad. Some fruit farmers often use cuttings for propagation, but the propagation speed of cuttings is slow and the survival rate is not high. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 李亚菲向太和李琳洁周世怡冯咪林幼荷
Owner HANGZHOU NORMAL UNIVERSITY