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Culture medium for Streptomyces actuosus fermentation production of nosiheptide, and culture method

A technology of active streptomyces and fermentation medium, which is applied in the field of active streptomyces fermentation to produce nosiheptide, can solve the problems of long fermentation period, high production cost, and lack of market competitiveness of products, so as to increase the fermentation unit and reduce the fermentation energy consumption effect

Inactive Publication Date: 2016-10-26
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2 There is a lack of literature reports on the complete fermentation process of nosiheptide in China, and the relevant literature reports are mainly based on experiments, which cannot provide effective technical support for large-scale production models
[0006] 3 The large-scale production of Nosiheptide has a long fermentation cycle, generally around 240h, and high energy consumption
[0007] 4 The comprehensive production cost of nosiheptide fermentation is relatively high, and the product lacks market competitiveness

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Seed culture: medium volume 10m 3 .

[0052] Dextran 40kg, glycogen 80kg, wort 60L, fish meal 90kg, cottonseed meal 110kg, corn gluten powder 130kg, ammonium sulfate 0.4kg, light calcium carbonate 10kg;

[0053] First sterilize the seed medium and cool it down to 20°C, and keep the pressure with sterile air. The quality of the sterilized seed medium is: amino nitrogen 32mg / L, dissolved phosphorus 101ug / ml, fat 6.2g / L; Under protection, the cultured active Streptomyces mother bottle fermentation broth was inserted into the seed medium according to the inoculum size of 20 L for cultivation. Seed culture conditions are: tank pressure 0.03-0.06MPa; tank temperature 28-29°C; air flow: 30m 3 / h; the stirring speed is 60r / min, until the cell concentration is 26%, and the culture time is 40h.

[0054] Fermentation culture: medium volume 100m 3 .

[0055] Dextran 600kg, glycogen 1000kg, wort juice 800L, fish meal 1100kg, cottonseed meal 1300kg, corn gluten meal 1400kg, silk...

Embodiment 2

[0070] Seed culture: medium volume 10m 3 .

[0071] Dextran 50kg, glycogen 90kg, wort 70L, fish meal 100kg, cottonseed meal 140kg, corn gluten powder 140kg, ammonium sulfate 0.5kg, light calcium carbonate 20kg;

[0072] First sterilize the seed medium and cool it down to 22°C, and keep the pressure with sterile air. The quality of the sterilized seed medium: amino nitrogen 35mg / L, dissolved phosphorus 111ug / ml, fat 7g / L; Next, the cultured active Streptomyces mother bottle fermentation liquid was inserted into the seed medium according to the inoculum size of 22 L for cultivation. The seed culture conditions are: tank pressure 0.03-0.06MPa; tank temperature 28-29°C; air flow: 35m 3 / h; the stirring speed is 65r / min, until the cell concentration is 27%, and the culture time is 41h.

[0073] Fermentation culture: medium volume 100m 3 .

[0074] Dextran 700kg, glycogen 1100kg, wort 900L, fish meal 1200kg, cottonseed meal 1400kg, corn gluten meal 1500kg, silkworm chrysalis pe...

Embodiment 3

[0089] Seed culture: medium volume 10m 3 .

[0090] Dextran 60kg, glycogen 100kg, wort 80L, fish meal 110kg, cottonseed meal 150kg, corn gluten powder 150kg, ammonium sulfate 0.6kg, light calcium carbonate 30kg;

[0091] First, sterilize the seed medium and cool it to 23°C, and keep the pressure with sterile air. The quality of the sterilized seed medium: amino nitrogen 39mg / L, dissolved phosphorus 122ug / ml, fat 8g / L; Next, the cultured active Streptomyces mother bottle fermentation broth was inserted into the seed medium according to the inoculum size of 25 L for cultivation. Seed cultivation conditions are: tank pressure 0.03-0.06MPa; tank temperature 28-29°C; air flow: 40m 3 / h; the stirring speed is 70r / min, until the cell concentration is 28%, and the culture time is 42h.

[0092] Fermentation culture: medium volume 100m 3 .

[0093] Dextran 800kg, glycogen 1200kg, wort juice 1000L, fish meal 1300kg, cottonseed meal 1500kg, corn gluten meal 1600kg, silkworm chrysalis...

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PUM

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Abstract

The present invention relates to a culture medium for Streptomyces actuosus fermentation production of nosiheptide, and a culture method. According to the present invention, the seed culture medium for Streptomyces actuosus fermentation production of nosiheptide, and the carbon source, the nitrogen source and optimal compatibility ratio in the fermentation culture medium are determined; and with the culture medium formula and the fermentation control process of the present invention, the nosiheptide fermentation unit can be increased, the fermentation cost can be reduced, the influence of the environment on the raw material source and the auxiliary material source can be minimized, the sufficient supply can be ensured, and the stable and efficient production of the nosiheptide can be achieved.

Description

technical field [0001] The invention belongs to the field of fermentation technology, and in particular relates to a culture medium and a culture method for fermenting and producing nosiheptide by active streptomyces. Background technique [0002] Nosiheptide, also known as Nosiheptide, is an antibiotic produced by active Streptomyces fermentation mode. At present, Nosiheptide has been included in the European Pharmacopoeia and the Japanese Feed Safety Law. Nosiheptide is used in many countries and regions. The Ministry of Agriculture of my country lists nosiheptide as a feed additive that can be added to feed for a long time. [0003] At present, relevant domestic literature reports the production process of Nosiheptide, and its fermentation mode is secondary fermentation or tertiary fermentation. The problem is: [0004] 1 The fermentation level of industrialized large-scale production is low, and its fermentation level is maintained at about 1500-1800ug / L. [0005] 2 T...

Claims

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Application Information

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IPC IPC(8): C12P21/02C12N1/20
CPCC12P21/02C07K5/06139C12N1/20
Inventor 任勇
Owner 宁夏泰瑞制药股份有限公司
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