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Preparation method of benzene boric acidification affinity chromatography stationary phase for chitosan oligosaccharide separation

A chromatographic stationary phase, chitosan oligosaccharide technology, applied in separation methods, chemical instruments and methods, sugar derivative preparation, etc., can solve the problem of unsatisfactory separation effect, small number of chromatographic columns, and difficulty in obtaining high-purity single-component chitosan oligosaccharides. Sugar and other problems, to achieve the effect of novel structure, good separation effect and high degree of separation

Active Publication Date: 2016-11-09
HARBIN INST OF TECH
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  • Application Information

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Problems solved by technology

[0003] The present invention will solve the problem that the existing chromatographic columns used for separating chitosan oligosaccharides are less, the separation effect is unsatisfactory, and it is difficult to obtain high-purity single-component chitosan oligosaccharides, and a kind of benzene for the separation of chitosan oligosaccharides is provided. Preparation method of borated affinity chromatography stationary phase

Method used

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  • Preparation method of benzene boric acidification affinity chromatography stationary phase for chitosan oligosaccharide separation
  • Preparation method of benzene boric acidification affinity chromatography stationary phase for chitosan oligosaccharide separation

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specific Embodiment approach 1

[0021] Specific embodiment one: a kind of preparation method of the phenylborated affinity chromatography stationary phase that is used for chitosan oligosaccharide separation described in this embodiment, specifically is carried out according to the following steps:

[0022] 1. Preparation of activated silica gel:

[0023] Add silica gel to hydrochloric acid solution with a volume percentage of 10% to 30%, and then reflux at a temperature of 100°C to 120°C for 5h to 8h, cool to room temperature after reflux, and wash the reacted silica gel with water until the washing liquid To be neutral, under the condition of temperature of 100℃~120℃, vacuum dry the washed silica gel for 8h~12h to obtain the activated silica gel;

[0024] The volume ratio of the mass of the silica gel to the volume percentage of 10%-30% hydrochloric acid solution is 1g:(20-50)mL;

[0025] 2. Preparation of chlorinated silica gel:

[0026] Put the activated silica gel in a round-bottomed flask, add N,N-di...

specific Embodiment approach 2

[0035] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the mass and volume percentage of the silica gel described in step 1 is 10% to 30% by volume of the hydrochloric acid solution, and the volume ratio is 1g: (35 ~ 50) mL . Others are the same as in the first embodiment.

specific Embodiment approach 3

[0036] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the mass ratio of activated silica gel described in step two to N,N-dimethylformamide is 1g:400mL . Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a preparation method of a chromatography stationary phase for chitosan oligosaccharide separation. The invention aims to solve the problems of a small number of chromatographic columns for chitosan oligosaccharide separation, unsatisfactory separation effect, and difficult acquisition of high purity single-component chitosan oligosaccharide. The method consists of: 1. preparing activated silica gel; 2. preparing chloro silica gel; 3. preparing benzene boric acidification silica gel, i.e. the benzene boric acidification affinity chromatography stationary phase for chitosan oligosaccharide separation. The invention provides the separation method of benzene boric acidification affinity chromatography stationary phase for chitosan oligosaccharide separation.

Description

technical field [0001] The invention relates to a preparation method of a chromatographic stationary phase for separating chitosan oligosaccharides. Background technique [0002] Chitooligosaccharide, also known as chitooligosaccharide, is a glucosamine oligomer with a degree of polymerization (DP) of 2-10 obtained by degrading chitosan through special treatment. Due to the short sugar chain of chitosan oligosaccharide, it has the characteristics of good solubility and low viscosity under physiological conditions. Unlike most polysaccharides, oligochitosan has free amino groups in its structure due to deacetylation, and is the only positively charged basic oligosaccharide in nature. These properties of chitosan oligosaccharides have attracted the attention of more and more researchers. A large number of literatures have reported the biological activities of chitosan oligosaccharides, and these biological activities are widely used in food, medicine, agriculture and environ...

Claims

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Application Information

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IPC IPC(8): B01J20/286B01J20/30B01D15/38C07H5/06C07H1/06C08B37/08
CPCB01D15/3804B01J20/286C07H1/06C07H5/06C08B37/003
Inventor 杨鑫孙敬明王静
Owner HARBIN INST OF TECH
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