Pharmaceutical composition of deoxycorticosterone acetate, and application of same to biological medicine
A technology of deoxycorticosterone acetate and its composition, which is applied in the field of biomedicine, can solve the problems that have not been reported related to deoxycorticosterone acetate, and achieve the effect of reducing the content and improving the yellowing effect
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Embodiment 1
[0017] Example 1: Compound (Ⅰ) isolation preparation and structure confirmation
[0018] Reagent sources: ethanol, petroleum ether, ethyl acetate, n-butanol, dichloromethane are analytically pure, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd., methanol, analytically pure, purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd.
[0019] Separation method: (a) Crush Forsythia (2kg), extract with 90% ethanol under hot reflux (20L×3 times), combine the extracts, concentrate until there is no alcohol smell (4L), and use petroleum ether (4L×3 times) in turn ), ethyl acetate (4L×3 times) and water-saturated n-butanol (4L×3 times) to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) step (a ) The medium n-butanol extract was removed with AB-8 type macroporous resin. First, eluted with 8% ethanol for 10 column volumes, and then eluted with 70% ethanol for 12 column volumes. Collect 70% eluate and depressurize Concentrate to obtain...
Embodiment 2
[0023] Example 2: Pharmacological effects
[0024] In this example, a one-time intragastric administration of 1-naphthyl isothiocyanate (ANIT) was used to replicate the rat jaundice model, and the drug-induced jaundice model rat serum alanine aminotransferase (ALT) and aspartate amino group were determined. The effect of transferase (AST) and total bilirubin (TBIL) content, observe the protective effect of the drug on the liver.
[0025] 1. Materials and methods
[0026] 1.1 Animals
[0027] Clean SD rats, both ♀♂, weighing 260~300g, purchased from the Experimental Animal Center of Kunming Medical College.
[0028] 1.2 Reagents and samples
[0029] Deoxycorticosterone acetate was purchased from the National Institute for the Control of Pharmaceutical and Biological Products. Compound (I) is self-made, and the preparation method is shown in Example 1. Bifendate dropping pills (Zhejiang Pharmaceutical Co., Ltd. Xinchang Pharmaceutical Factory, specification 1.5mg / capsule); 1-naphthyl is...
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