A method for separating and purifying protostructural anthocyanins in Daxinganling wild blueberry by using membrane

Abstract

The invention discloses a method for purifying primary structure anthocyanin of wild blueberries from Great Khingan by membrane separation. The method comprises the following steps: pre-treating a wild blueberry raw material; acidifying alcohol, and performing water bath extraction by adopting acidified alcohol, filtering and centrifuging to prepare an anthocyanin extract; filtering by adopting a filter membrane; adsorbing by utilizing pre-treated polyamide adsorption resin; performing elution chromatography; performing refined filtration by adopting a refined filter membrane; performing ultrafiltration by adopting an ultrafiltration membrane; concentrating at reduced pressure; drying by spraying and refrigerating to obtain crystal or pasty blueberry anthocyanin. The method can be used for reducing the extraction cost of blueberry anthocyanin and improving the extraction rate which is higher than 40mg / g, and is easy to control and simple in operation. According to the method, the resin can be repeatedly used after being eluted so as to reduce cost, and the problem of low extract purity caused by the fact that the resin has weak selectivity for adsorbed materials can be overcome by means of refined filtration and ultrafiltration.

Description

technical field [0001] The invention belongs to the technical field of extraction methods, in particular to a method for separating and purifying the anthocyanins of the Daxing'anling wild blueberry by using a membrane, and belongs to the field of natural organic chemistry. Background technique [0002] Anthocyanins, also known as anthocyanins, are a class of water-soluble natural pigments that widely exist in plants. They belong to flavonoids and mostly exist in the form of glycosides, also known as anthocyanins. The earliest and most abundant anthocyanin is red grape skin extracted from red grape pomace, which was commercially available in Italy in 1879. As a natural food coloring, anthocyanins are safe, non-toxic, rich in resources, and have certain nutritional and pharmacological effects. They have great application potential in food, cosmetics, and medicine. [0003] Blueberry, also known as Dusi lingonberry, belongs to Rhododendronaceae and Vaccinium genus. Its fruit...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the existing technical problems, the present invention provides a method for separating and purifying the original structure anthocyanins in Daxinganling wild blueberry by using membrane, which can reduce the extraction cost of blueberry anthocyanins and improve the extraction rate. Using the extraction method of the present invention, blueberry flower The extraction rate of cyanin can be higher than 40 mg / g. This method is easy to control, simple to operate, and can be used repeatedly after the resin is eluted, saving costs. The selectivity is not strong and the purity of the extract is not high

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