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Urechis unicinctus visceral protein source zinc chelating peptide

A single-ring thorn and chelating peptide technology, applied in the direction of peptides, can solve the problem of wasting fishery resources

Inactive Publication Date: 2016-11-09
MARINE FISHERIES RES INST OF ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

People's traditional practice is to eat only the body wall and discard the internal organs, wasting precious fishery resources

Method used

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  • Urechis unicinctus visceral protein source zinc chelating peptide
  • Urechis unicinctus visceral protein source zinc chelating peptide
  • Urechis unicinctus visceral protein source zinc chelating peptide

Examples

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Embodiment

[0027] 1) Pretreatment of the viscera of C. monocircleum: chop up the viscera of C. monocircleum and use a tissue blender to homogenize it. The acetone was stirred overnight to degrease, the supernatant was discarded by centrifugation, the precipitate was dried in a blast drying oven at 40°C overnight and then pulverized to obtain the viscera powder of C. monocylicus.

[0028] ) Preparation of the visceral protein of C. unicycle: Take the viscera powder of C. unicycle at a ratio of 1:20 (mass volume ratio, 50 g: 1000 mL), add distilled water, heat in a constant temperature water bath at 80°C for 4 hours, centrifuge and take the supernatant solution, repeat the above steps three times, and finally obtain the supernatant as the experimental sample, which is concentrated and freeze-dried.

[0029] ) Enzymatic hydrolysis of the visceral protein of E. monocylicus: add the sample to distilled water at a ratio of 1:5 (mass volume ratio, 10 g: 50 mL), then adjust the pH to 6.8, add 2%...

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Abstract

The invention discloses an urechis unicinctus visceral protein source zinc chelating peptide; the zinc chelating peptide has an amino acid sequence of Phe-Pro-Tyr-Lys-His, and the ESI-MS detection molecular weight is 690.78 Da. During preparation, utrechis unicinctus viscera are used as a raw material, and the product is obtained through viscera pretreatment, protein extraction, enzyme hydrolysis, gel purification and chromatography; the zinc chelating peptide provides a technical support for development of zinc supplemental health-care food and drugs, and also provides a new idea for high-valued utilization of the urechis unicinctus viscera.

Description

technical field [0001] The invention relates to an animal visceral active polypeptide, in particular to a zinc chelating peptide derived from the visceral protein of monocyclic echinacea. Background technique [0002] Active biological peptides are favored by more and more consumers due to their wide range of biological activities and their unique effects in medicine, health care products, food and cosmetics, making them a popular choice in the food industry, health care product industry, and daily chemical industry. Competing for the center of attention. Zinc is an essential trace element for the human body, and its deficiency can lead to diseases such as poor growth and development, mental retardation, and decreased immunity in children. Traditional zinc supplements (such as zinc gluconate and zinc citrate) have low absorption and utilization rates, cause severe gastrointestinal irritation, and are not easy to take for a long time. The chelate salt formed by zinc and met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06
CPCC07K7/06
Inventor 李佩佩张小军陈思龙举陈荫
Owner MARINE FISHERIES RES INST OF ZHEJIANG