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L-valine fermentation medium and its fermentation method for producing L-valine

A fermentation medium and fermentation method technology, applied in the field of biological fermentation engineering, can solve the problems of unsatisfactory, low sugar-acid conversion rate at production level, etc., and achieve the effects of low production cost, improved yield and sugar-acid conversion rate, and simple process

Active Publication Date: 2019-12-13
HEBEI SHENGXUE DACHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production of L-valine by microbial fermentation has a relatively low domestic production level and sugar-acid conversion rate, which is far from meeting the needs of the domestic market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh 20g of cane molasses, 40g of corn steep liquor, 20g of ammonium sulfate, 2g of potassium dihydrogen phosphate, 1.0g of magnesium sulfate, 0.02g of ferrous sulfate, 0.02g of manganese sulfate, 0.0004g of vitamin H, 0.0004g of vitamin B1, and drink water to volume To 2L, put it into a 5L fermenter, sterilize at 115°C, insert the seed culture, the inoculum size is 10%, the tank pressure is 0.01MPa, the rotation speed is 200rpm, the pH is 6.7, and the temperature is 25°C. According to the amount of residual sugar, add 80% glucose solution to control the residual sugar content at 20-25g / L. During the fermentation process, the pH is controlled between 6.7-7.0 by ammonia water. Control dissolved oxygen in stages: 20% for 0-36h, 5% for 36-70h. After 70 hours of fermentation, the fermentation unit of L-valine was 77g / L, and the sugar-acid conversion rate was 33.6%.

Embodiment 2

[0023] Weigh 120g of cane molasses, 100g of corn steep liquor, 40g of ammonium sulfate, 10g of potassium dihydrogen phosphate, 4g of magnesium sulfate, 0.1g of ferrous sulfate, 0.1g of manganese sulfate, 0.002g of vitamin H, 0.002g of vitamin B1, and dilute drinking water to 2L, put into a 5L fermenter, after sterilizing at 125°C, insert the seed culture, the inoculum size is 18%, the tank pressure is 0.05MPa, the rotation speed is 200rpm, the pH is 7.0, and the temperature is 38°C. According to the amount of residual sugar, add 80% glucose solution to control the residual sugar content at 20-25g / L. During the fermentation process, the pH is controlled between 6.7-7.0 by ammonia water. Control dissolved oxygen in stages: 20% for 0-36h, 5% for 36-70h. After 70 hours of fermentation, the fermentation unit of L-valine was 81g / L, and the sugar-acid conversion rate was 36.08%.

Embodiment 3

[0025] Weigh 56g of cane molasses, 50g of corn steep liquor, 24g of ammonium sulfate, 6g of potassium dihydrogen phosphate, 1.6g of magnesium sulfate, 0.08g of ferrous sulfate, 0.08g of manganese sulfate, 0.0014g of vitamin H, 0.0014g of vitamin B1, and drink water to volume To 2L, put into a 5L fermenter, after sterilizing at 121°C, insert the seed culture, the inoculum size is 15%, the tank pressure is 0.02MPa, the rotation speed is 200rpm, the pH is 6.7, and the temperature is 32°C. According to the amount of residual sugar, add 80% glucose solution to control the residual sugar content at 20-25g / L. During the fermentation process, ammonia water is used to control pH between 6.7-7.0; dissolved oxygen is controlled in stages: 20% for 0-36h, 5% for 36-70h; 70h of fermentation is put into the tank, and the fermentation unit of L-valine is detected to be 80g / L, the sugar-acid conversion rate is 35.21%.

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PUM

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Abstract

The invention provides an L-valine fermentation medium and a fermentation method for producing L-valine with the same. The fermentation method is simple in process and low in production cost, has the effect of increasing the yield and conversion rate of glucose of L-valine, and is suitable for industrial production. The fermentation medium comprises cane molasses, corn steep liquor, ammonium sulfate, ammonia water, monopotassium phosphate, magnesium sulfate, ferrous sulfate, manganese sulfate, vitamin H and vitamin B1. The fermentation medium and the fermentation method have the beneficial effects that the fermentation medium takes cane molasses as a carbon source and corn steep liquor, ammonium sulfate and ammonia water as nitrogen sources and combines with a feeding process and the mode of controlling dissolved oxygen by segments to carry out L-valine fermentation; and the fermentation unit of obtained L-valine can be 93g / L, and the conversion rate of glucose of L-valine is 40.35%, thus obviously increasing the yield and conversion rate of glucose of L-valine.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, and in particular relates to an L-valine fermentation medium and a fermentation method for producing L-valine using it. Background technique [0002] L-valine (L-valine) was discovered from casein protein in 1901, the chemical name is L-α-aminoisovaleric acid, and the molecular formula is C 5 h 11 NO 2 , relative molecular weight 117.15, white crystal or crystalline powder, odorless, bitter taste. L-valine is a branched-chain amino acid, one of the eight essential amino acids for the human body, and has a variety of physiological functions. L-valine is the basic unit of protein molecules and one of the amino acids necessary for animal nutrition. The basic raw materials for the synthesis of hormones, hormones and enzymes, etc., play a special physiological function in the human body, as an important substance converted into ATP to maintain life characteristics. L-va...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/15
CPCC12P13/08
Inventor 李猛王婷魏伟林青
Owner HEBEI SHENGXUE DACHENG PHARMA