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Method for culturing sterile paddy seedlings

A cultivation method and technology of aseptic seedlings, applied in the field of plant tissue culture, can solve the problems of increased workload, damage to seed embryos, cumbersomeness, etc., and achieve the effect of easy operation and simple method

Inactive Publication Date: 2016-11-16
CHANGSHA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of modern research technology, there are relatively few methods for disinfecting rice seeds. At present, the method with better effect is only Zhou Yingjun and others who first shell the rice seeds and then disinfect them with 5% sodium hypochlorite for 45 minutes. This method is not only cumbersome , increases the workload, and will damage the embryo of the seed and affect the germination rate, so it is more important to find a disinfection method suitable for rice seeds

Method used

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  • Method for culturing sterile paddy seedlings
  • Method for culturing sterile paddy seedlings
  • Method for culturing sterile paddy seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 5 grams of rice seeds, place it in a beaker, fill the beaker with water, then pull out the rice grains, and let the water soak for 4 hours. At the same time, 5 sets of petri dishes and 3 bottles of 300ml deionized water were sterilized at 121°C for 30 minutes. 40 minutes before the experiment, put the required equipment for the experiment, such as: tweezers, alcohol lamp, sterile potato medium plate, etc. Transfer the soaked rice seeds to a sterilized petri dish in a clean workbench, then add alcohol with a concentration of 75% to the petri dish to cover the rice seeds, pour off the alcohol after soaking for 1 minute, and pour it into the container. Add an appropriate amount of formaldehyde with a concentration of 40% to the petri dish with seeds to make it cover the seeds, pour off the formaldehyde after soaking for 5 minutes, and then wash it three times with sterile water. Then clamp 15 seeds with aseptic tweezers and place them in three sterile potato medium ...

Embodiment 2

[0028] Take by weighing 10 grams of rice seeds, place it in a beaker, fill the beaker with water, then pull out the empty husks of the rice, and let the water soak for 4 hours. At the same time, sterilize one 500ml beaker and five bottles of 300ml deionized water at 121°C for 30min. 40 minutes before the experiment, put the required equipment for the experiment, such as: tweezers, alcohol lamp, sterile polyethylene high-temperature-resistant culture cup with wet gauze inside, etc. After the sterilization is completed, transfer the soaked rice seeds to a sterilized beaker in the ultra-clean workbench, then add alcohol with a concentration of 70% to the beaker to cover the rice seeds, soak for 2 minutes and then pour it out Alcohol, and then add an appropriate amount of formaldehyde with a concentration of 40% to the beaker containing the seeds to make it submerge the seeds, pour off the formaldehyde after soaking for 5 minutes, and then wash it three times with sterile water. ...

Embodiment 3

[0030] Take by weighing 10 grams of rice seeds, place it in a beaker, fill the beaker with water, then fish out the empty husks of the rice, and let the water soak for 8 hours. At the same time, sterilize one 500ml beaker and five bottles of 300ml deionized water at 121°C for 30min. 40 minutes before the experiment, put the required equipment for the experiment, such as: tweezers, alcohol lamp, sterile polyethylene high-temperature-resistant culture cup with wet gauze inside, etc. After the sterilization is completed, transfer the soaked rice seeds to a sterilized beaker in the ultra-clean workbench, then add alcohol with a concentration of 75% to the beaker to cover the rice seeds, soak for 1 minute and then pour it out Alcohol, and then add an appropriate amount of formaldehyde with a concentration of 38% to the beaker containing the seeds to make it submerge the seeds, pour off the formaldehyde after soaking for 5 minutes, and then wash it three times with sterile water. T...

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Abstract

The invention discloses a method for culturing sterile paddy seedlings and relates to the technical field of plant tissue culture. The method particularly includes: taking paddy seeds, and soaking the paddy seeds; preparing a culture cup, padding four layers of wet gauze at the bottom of the culture cup, and sterilizing for 25-35 min; putting the paddy seeds after being soaked in a sterile beaker, pouring alcohol into the beaker, and allowing the alcohol to soak for 1-2 min; pouring out the alcohol, adding formaldehyde into the beaker, and allowing formaldehyde to soak the seeds for 3-5 min; pouring out formaldehyde, adding a proper amount of sterile water into the beaker to clean the seeds, putting the seeds in the sterile culture cup padded with the gauze after the seeds are cleaned well, sealing the sterile culture cup through a sterile culture membrane, and putting the culture cup in an environment at temperature of 25-32 DEG C for accelerating germination. By using the method, the sterilizing problem of paddy seeds in existing labs can be solved, so that pretreatment technology is provided for conducting subsequent tissue culture and transgenic work, and an experimental material is provided for studying paddy organic nutrition and root exudate.

Description

Technical field: [0001] The invention relates to a method for cultivating sterile rice seedlings, belonging to the technical field of plant tissue culture. Background technique: [0002] The "sterile seedling" method is an extension of plant tissue culture technology. Aseptic seedlings are necessary biological materials for experiments on plant organic nutrition and root exudates, and the effective cultivation of rice sterile seedlings is the key to carrying out subsequent tissue culture and transgenic work. premise and basis. Disinfection of rice seeds is a troublesome task. This is because there are many endophytic bacteria in rice seeds, and the seeds are wrapped by a shell that is tightly combined with the embryo, which brings trouble to disinfection and makes the disinfection solution difficult. Penetrates into seeds to kill microorganisms. In the field of modern research technology, there are relatively few methods for disinfecting rice seeds. At present, the method ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01C1/08
CPCA01C1/08A01H4/00
Inventor 唐建洲龚亮何昊城朱天彧张志元
Owner CHANGSHA UNIVERSITY
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