Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of tissue culture method of candy iris

A technology of candy iris and cultivation methods, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of large plant variability, poor plant type consistency, and failure to maintain excellent traits, and achieve excellent results and good conditions , callus compact effect

Active Publication Date: 2018-04-24
BEIJING FORESTRY UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The propagation method of iris is usually by seeds, and it can also be propagated by division. However, the traditional method of propagation has poor consistency of plant type, large plant variability, and excellent traits cannot be maintained.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of tissue culture method of candy iris
  • A kind of tissue culture method of candy iris
  • A kind of tissue culture method of candy iris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Present embodiment relates to a kind of tissue culture method of candy iris, comprising the steps:

[0042] (1) explant disinfection sows the seed of candy iris, obtains sowing seedling, collects robust receptacle without damage by disease and insect as the explant of tissue culture (as figure 1 ), soak the collected explants in 20% detergent water for 30 minutes, and then rinse them with running water for 1-2 hours; disinfect them with 70% absolute ethanol on the ultra-clean workbench for 30 seconds, and rinse them with sterile water for 30 seconds. Then disinfect with 2% sodium hypochlorite for 12 minutes, rinse with sterile water 5 times;

[0043] (2) Induction and differentiation of adventitious buds Inoculate the sterilized explants on the induction medium, induction medium: MS+6-BA 1.0mg·L-1+2,4-D 1.0mg·L-1, Send it to the culture room for sterile culture. The conditions of the culture room: temperature is 25°C, light time is 12h·d-1, light intensity is 25-30μmol...

Embodiment 2

[0048] Present embodiment relates to a kind of tissue culture method of candy iris, comprising the steps:

[0049] (1) explant disinfection sows the seed of candy iris, obtains sowing seedling, gathers the explant that the stem node that does not have damage by disease and pest is strong as the explant of tissue culture, the explant that gathers is earlier soaked with 20% detergent water 30 minutes, then rinse with running water for 1-2 hours; disinfect with 70% absolute ethanol for 30 seconds on an ultra-clean workbench, rinse with sterile water for 3 times, then disinfect with 2% sodium hypochlorite for 12 minutes, and rinse with sterile water for 5 times;

[0050] (2) Induction and differentiation of adventitious buds Inoculate the sterilized explants on the induction medium, induction medium: MS+6-BA 1.0mg·L-1+2,4-D 1.0mg·L-1, Send it to the culture room for sterile culture. The conditions of the culture room: temperature is 25°C, light time is 12h·d-1, light intensity is ...

Embodiment 3

[0055] Present embodiment relates to a kind of tissue culture method of candy iris, comprising the steps:

[0056] (1) explant disinfection and sowing the seeds of candy iris, obtain seedlings, collect strong receptacles without damage by diseases and insect pests as the explants of tissue culture, soak the explants collected for 30min with 20% detergent water earlier , then rinse with running water for 1 to 2 hours; disinfect with 70% absolute ethanol for 25 seconds on an ultra-clean workbench, rinse with sterile water for 3 times, then disinfect with 2% sodium hypochlorite for 14 minutes, and rinse with sterile water for 5 times;

[0057] (2) Induction and differentiation of adventitious buds Inoculate the sterilized explants on the induction medium, induction medium: MS+6-BA 0.8mg·L-1+2,4-D 1.2mg·L-1, Send it to the culture room for sterile culture. The conditions of the culture room: temperature is 25°C, light time is 12h·d-1, light intensity is 25-30μmol·m-2·s-1, after 4 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a tissue culture method of Pardancanda norrisii. The method comprises steps as follows: 1) flower buds, receptacles, nodes or tender leaves of Pardancanda norrisii are selected as explants, a callus induction medium is inoculated with the explants, and culture is performed until adventitious buds are differentiated; 2) the adventitious buds are transplanted in a subculture multiplying medium until seedlings are differentiated; 3) the seedlings are transplanted in a rooting medium, and Pardancanda norrisii seedlings are obtained after culture and rooting. With the tissue culture method, good traits of Pardancanda norrisii can be kept, large-scale rapid breeding can be realized, the callus formation rate is high in the culture process, the growth state is good, and the final survival seedling rate is high.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a tissue culture technique of candy iris. Background technique [0002] Candy Iris (×Pardancanda norrisii) is a collective name for offspring populations obtained from wild Iris (Iris dichotoma) and Shegan (Iris domestica) through continuous crossing and backcrossing. Because the perianth will roll up the next day after the flower closes, and the ovary is like candy, so it is called Candy lily, which is translated as candy iris in Chinese. Both Shegan and Wild Iris are native to my country. They bloom in summer when there are few flowers, and the flowering period lasts for one and a half months. They have excellent resistance to drought, cold, and barrenness, and are very good breeding materials. But the color is relatively single, which limits their application in gardens. The hybrid offspring Candy Iris obtained through interspecific hybridization has a ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 高亦珂阮丽丽刘蓉杨占辉郭彦超张启翔王佳
Owner BEIJING FORESTRY UNIVERSITY