Composting method capable of regulating microbial community structure of unculturable microorganisms
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A technology of microbial flora and composting method, which is applied to the treatment of biological organic parts, organic fertilizers, fertilization devices, etc. Effect
Inactive Publication Date: 2016-11-16
NORTHEAST AGRICULTURAL UNIVERSITY
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[0005] The purpose of the present invention is to solve the problem that difficult-to-cultivate microorganisms are difficult to regulate because they cannot be separated and cultivated, and to effectively improve the quality of compost, and provide a composting method for regulating the structure of difficult-to-cultivate microorganisms
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specific Embodiment approach 1
[0020] Specific embodiment one: the composting method of this embodiment difficult to cultivate microbial flora structure regulation is carried out according to the following steps:
[0021] 1. Take a small amount of compost raw materials for composting, and then collect samples from the four stages of compost heating period, high temperature period, cooling period and decomposing period, and perform PCR-DGGE identification on the samples in these four stages, and then analyze the conditions of the dominant bacteria in the samples. The bands were sequenced, and the physicochemical properties of samples in different periods were obtained;
[0022] 2. Use RDA to analyze the key control factors of different bacterial flora structures, and determine the control range of key factors for different microorganisms;
[0023] 3. According to the target requirements of composting, determine the target functional strains of composting, then compost and adjust the composting parameters acc...
specific Embodiment approach 2
[0026] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the physical and chemical properties obtained in step 1 include the carbon-to-nitrogen ratio, moisture, temperature, pH, germination index, water-soluble organic carbon, and water-soluble organic nitrogen of the sample. , ammonia nitrogen, nitrate nitrogen and organic matter content. Other steps and parameters are the same as those in Embodiment 1.
specific Embodiment approach 3
[0027] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in step 3, the carbon metabolism, nitrogen metabolism, phosphorus metabolism and humification process of the composting target functional strain are controlled by regulating the composting parameters. Other steps and parameters are the same as those in Embodiment 1 or 2.
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Abstract
The invention discloses a composting method capable of regulating a microbial community structure of unculturable microorganisms, relates to a composting method and solves the problem that the activity of the unculturable microorganisms is difficult to regulate due to failure of isolated culture to further effectively improve the composting quality. The composting method comprises steps as follows: 1, a few raw materials are taken for composting, samples at different stages are collected for PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) recognition and sequencing, and the physicochemical properties of the samples are acquired; 2, R key control factors of different microbial community structures are analyzed through RDA (representational difference analysis), and the regulation range of the key control factors is determined for different microorganisms; 3, a composting target function strain is determined according to requirements of composting targets, composting is performed, composting parameters are regulated correspondingly, and the composting process is controlled. The succession laws of microbial community structures of microorganisms in different composting natural environments can be rapidly and accurately analyzed, the response relation between environment factors and the microorganisms in the composting process is determined, and a process regulation technology in the composting environment is realized.
Description
technical field [0001] The invention relates to a composting method. Background technique [0002] With the progress and development of society, the amount of organic solid waste produced by human beings in production and life is increasing day by day. The main organic solid wastes include crop stalks, livestock and poultry manure, and domestic waste. At present, the treatment methods for organic solid waste are mainly sanitary landfill, incineration and composting. [0003] Aerobic composting is the most effective method to realize the recycling and harmless treatment of organic solid waste. The essence of aerobic composting is mainly to use the rapid succession of microbial communities in the community structure to act synergistically on the compost substrate, and to treat organic solid waste through the physiological and biochemical processes of microbial communities. The microbial colony in the pile has a direct impact on the decomposition rate of the pile and the deg...
Claims
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