High or low expression sites in Saccharomyces cerevisiae

Abstract

The invention belongs to the field of genetic technology, and specifically relates to determination of a site effect of saccharomyces cerevisiae as well as screening of gene high-expression or low-expression sites, in particular to a series of gene high-expression or low-expression sites in the saccharomyces cerevisiae as well as vectors for interpolating genes in the high-expression sites and low-expression sites. According to the invention, the site effect of the saccharomyces cerevisiae is researched at a whole genome level, the distribution law of the site effect in saccharomyces cerevisiae genome is revealed, and the high-expression sites of the saccharomyces cerevisiae such as YBR128C, YDR448W, YGR240C, YHR142W, YML059C, YPL014W and YPR028W and the low-expression sites of the saccharomyces cerevisiae such as YBR001C, YGR038W, YIL092W, YLR371W and YPL241C are screened and obtained; therefore, a rational design basis is provided for the expression of exogenous metabolites and by-products by taking saccharomyces cerevisiae cells as chassis cells.

Description

technical field [0001] The invention belongs to the field of gene technology, and specifically relates to the determination of the site effect of Saccharomyces cerevisiae, and the screening of high-expression or low-expression sites of genes, in particular to a series of high-expression or low-expression sites of genes in Saccharomyces cerevisiae and High expression sites and low expression sites are inserted into the gene vector. Background technique [0002] Saccharomyces cerevisiae is one of the main model organisms in microbial research and is often used to express foreign proteins. Researchers usually use biotechnology to introduce exogenous genes or even a series of metabolic pathways into Saccharomyces cerevisiae cells, thereby synthesizing some natural products, including important pharmaceutical and chemical products such as terpenoids and polyunsaturated fatty acids. [0003] There are generally two ways to introduce exogenous genes and metabolic pathways, one is ...

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Problems solved by technology

However, the above studies are limited to individual sites or a certain chromosome, and cannot reveal the distribution and mechanism of site effects in the Saccharomyces cerevisiae genome from a global perspective.

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