Construction method of animal experimental model for verifying food blood glucose generation index and animal model constructed through method

Abstract

The invention discloses a construction method of an animal experimental model for verifying a food blood glucose generation index and the animal model constructed through the method. The construction method comprises the steps that at least ten female rats and at least ten male rats are selected from wistar rats and SD rats, the rats are not fed on food and fed on water for 10 hours to 16 hours, and the intragastric administration food amount is calculated through weighing; the fasting blood glucose is detected by adopting a Roche glucometer, blood glucose values obtained 30 min, 60 min, 90 min and 120 min after intragastric administration of glucose, maltose, soft sugar, saccharose, lactose and fructose is conducted are detected, and the area under a food blood glucose response curve of all time detection points of each rat in each group is calculated by adopting an integral method; a glucose experiment result is taken as the GI standard 100, and GI values of other food are calculated according to the following formula: GI=100*the area under a blood glucose response curve of the food to be verified / the area of a blood glucose response curve of glucose; trend comparison is conducted on the sequence of the food GI values verified from the rats and the sequence of the GI values verified from a human body, and 7-10-week-age SD male rats with the weight of 210 g to 350 g are taken as the animal experimental model for verifying the food blood glucose generation index.

Description

technical field [0001] The invention relates to the technical field of animal experimental model construction, in particular to a method for constructing an animal experimental model for quickly and efficiently testing and verifying the glycemic index of food and the animal model constructed therefor. Background technique [0002] In recent decades, with the development of the global economy and the transformation of nutritional lifestyle, type 2 diabetes has become one of the most serious chronic non-communicable diseases that endanger human health. According to the data released by the International Diabetes Federation in 2012, there are more than 371 million people with diabetes in the world, among which, the number of people with diabetes in my country ranks first in the world and is showing an upward trend. Type 2 diabetes not only directly affects the quality of life of patients, but its complications also pose a huge threat to the health of patients. Existing studies...

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Problems solved by technology

[0006] In view of the demand and technical status of the determination of food glycemic index, the first object of the present invention is to provide a method for constructing an animal experimental model capable of quickly and efficiently testing and verifying food glycemic index; the second object is to provide a method for To replace the human body to verify the animal model of food glycemic index, to solve the dilemma of human food glycemic index measurement, and to contribute to the prevention and treatment of type 2 diabetes

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