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Culture method of pure-line porphyra yezoensis

A technology of Porphyra zebra and its cultivation method, which is applied in the field of plant cultivation, can solve the problem of not being able to ensure that the cut pieces come from parts with consistent genetic composition, and achieve the effects of avoiding genetic mixing and simple operation process

Inactive Publication Date: 2016-11-23
南通中国科学院海洋研究所海洋科学与技术研究发展中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Filaments can be obtained by cutting a small piece from a single plant's fronds and culturing them separately, but since it cannot be guaranteed that the cut pieces come from parts with the same genetic composition, the obtained filaments are not necessarily pure lines

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for cultivating pure line Porphyra variegata, comprising the following steps:

[0032] 1. Scrub the shells inoculated with Porphyra variegata filaments, put them face up in a clean beaker, first induce them at 24-26°C for 15-20 days, change the water once a week and brush the shells to induce shell silk Swell the body, then lower the temperature to 20°C and stimulate for 3-5 days to release the conchospores. During the induction period, take the shells every morning and drain the water on the surface for observation to determine whether there are conchospores scattered. Wait until more spores are observed. proceed to the next step;

[0033] 2. Pour the induced release of ascospores into a high-temperature sterilized plate, add seawater with PES nutrients, and cultivate them at 18°C ​​for about three weeks, changing the water once a week until they grow into ascospore seedlings. 2500-3000lux, photoperiod 12h / 12h (day / night);

[0034] 3. Pick out a single ascos...

Embodiment 2

[0039] A method for cultivating pure line Porphyra variegata, comprising the following steps:

[0040] 1. Put the free filaments of Porphyra zebra in a high-temperature sterilized Erlenmeyer flask, add sterilized seawater containing PES medium, change the water once a week, and pass through the air filtered by copper sulfate during the cultivation process, 24- Induce at 26° C. for 15-25 days to make the free filaments expand, then cool down to 20° C. and stimulate for 3-5 days (stop aeration at this time) to induce the release of ascospores from the free filaments.

[0041]2. The obtained conchospores grow attached to the net rope. The growth conditions are seawater with PES nutrients added, the water is changed once a week, the temperature is 18°C, the light intensity is 2500-3000lux, and the photoperiod is 12h / 12h (day / night) , cultivated for about three weeks until they grow into ascospore seedlings.

[0042] 3. Pick individual conchospore seedlings on the seedling rope an...

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Abstract

The invention discloses a culture method of pure-line porphyra yezoensis. The culture method comprises the following steps: 1) culturing porphyra yezoensis filament, and inducing the porphyra yezoensis filament to release conchospores; 2) culturing the conchospores; 3) cultivating the conchospore seedlings to grow monospore seedlings; 4) cultivating mother seedlings to continuously diffuse monospore seedlings; 5) cultivating the monospore seedlings to grow into large porphyra thalli, namely pure-line thalli consistent in genetic material; and 6) cultivating the pure-line thalli till sexual maturity to form carpospore cysts, and germinating the diffused carpospores to form filament, namely pure-line filament. According to the invention, all monospore seedlings are derived from the same monospore, homozygous filament can be obtained for sure, and the method is a fast and simple method for obtaining pure-line filament and is essential to the cultivation of good varieties of porphyra yezoensis.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to a method for cultivating pure Porphyra variegata. Background technique [0002] Laver has important economic value and is one of the main economic seaweeds. According to statistics, the annual output of laver is about 1.8×10 4 tons of dry products, the annual output value is estimated to be about 2 billion US dollars. The main varieties of laver cultivated in my country are Porphyra altar and Porphyra variegata, among which Porphyra altar is mainly cultivated in the south of the Yangtze River (Fujian Province and Guangdong Province), and Porphyra variegata is mainly cultivated in the north of the Yangtze River (Jiangsu Province and Shandong Province, mainly in the Nantong, Yancheng and Lianyungang sea areas). Porphyra zebra has thinner algae and better taste. Although its yield is not as good as that of algae, its economic value is higher than that of algae. [0003] The culti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N3/00C12R1/89
CPCC12N1/12C12N3/00
Inventor 王广策黄爱优何林文
Owner 南通中国科学院海洋研究所海洋科学与技术研究发展中心
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