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A method of measuring the EGCG content in tea polyphenols

A detection method, the technology of tea polyphenols, applied in the field of detection of EGCG content in tea polyphenols, can solve problems such as lack of quality control, and achieve the effects of symmetry, good peak shape, high sensitivity, and moderate retention time

Inactive Publication Date: 2016-11-30
JIANGSU TIANSHENG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] EGCG is one of the main components in tea polyphenols. The inventors of the present invention have found that there is no effective detection method for quality control at present. To research and develop a detection method suitable for routine detection of EGCG content appears to be particularly important

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  • A method of measuring the EGCG content in tea polyphenols
  • A method of measuring the EGCG content in tea polyphenols
  • A method of measuring the EGCG content in tea polyphenols

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Embodiment 1

[0030] Detection method

[0031] (1) Set high-performance liquid chromatography conditions: the chromatographic column is Agilent Eclipse XDB-C18: 4.6 × 250mm, 5um, with a concentration of 0.1% aqueous acetic acid as mobile phase A, and a volume ratio of 60:40 methanol-acetonitrile as Mobile phase B, the elution method is gradient elution, the flow rate is 1.0mL min -1 , the column temperature is 35°C, the detection wavelength is 278nm, the injection volume is 20μl, and the volume ratio of mobile phase A and B in the liquid phase is 90:10 from 0 minutes onwards; 75:25 from 20 minutes onwards; 35 minutes onwards It is 90:10; the number of theoretical plates should not be less than 2000 according to EGCG calculation.

[0032] (2) Preparation of reference substance solution: Weigh 20 mg of EGCG reference substance, accurately weigh it, place it in a 50ml volumetric flask, add mobile phase A, dilute to the mark, shake well, and make the concentration 0.400mg·ml -1 solution, as a...

Embodiment 2

[0036] In the setting of high performance liquid chromatography conditions: the concentration is 0.2% acetic acid aqueous solution as mobile phase A, the volume ratio is 70:30 methanol-acetonitrile as mobile phase B, the elution method is gradient elution, and the flow rate is 1.2mL. min -1 , the column temperature was 40° C., the detection wavelength was 281 nm, and the injection volume was 15 μl. Other steps are identical with embodiment 1.

Embodiment 3

[0038] In the setting of high performance liquid chromatography conditions: the concentration is 0.15% acetic acid aqueous solution as mobile phase A, the volume ratio is 65:35 methanol-acetonitrile as mobile phase B, the elution method is gradient elution, and the flow rate is 0.8mL. min -1 , the column temperature was 20° C., the detection wavelength was 275 nm, and the injection volume was 10 μl. Other steps are identical with embodiment 1.

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Abstract

A method of measuring the EGCG content in tea polyphenols is disclosed. The method includes (1) a step of setting high performance liquid chromatography conditions, (2) a step of preparing a contrast solution, (3) a step of preparing a solution of a sample to be detected, and (4) a step of measuring the content, namely a step of separately measuring the contrast solution and the solution of the sample to be detected, injecting into a liquid chromatograph, performing operation of the high-efficient liquid chromatograph, recording chromatograms and calculating the EGCG content by utilizing the chromatograms. The method adopts a gradient elution manner, and can effectively separate EGCG and EC which are two components difficult to separate in the tea polyphenols. The method is simple, convenient, high in sensitivity, good in reappearance, good in sample separation degree, good in symmetry, good in peak shapes, proper in retention time and more suitable for routine detection, and can be effectively used for EGCG content detection and quality control.

Description

technical field [0001] The invention relates to a method for detecting compounds, in particular to a method for detecting EGCG content in tea polyphenols. Background technique [0002] Cancer has become the number one killer threatening human existence in the 21st century, with an average of more than 6 million people dying of various cancers every year. China has become one of the countries with high incidence of cancer in the world, with more than 2 million new cancer patients every year, accounting for 20% of the global total, and showing a rapid growth trend every year. Clinically, the current drugs for treating cancer are mainly chemical drugs, but due to the high toxicity of chemical drugs, while killing tumor cells, they also non-selectively kill autoimmune cells such as white blood cells and lymphocytes, resulting in various complications and serious diseases. Adverse reactions, and long course of treatment, high cost, there are greater limitations in clinical use. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 季浩徐娟阚建伟于燕燕孔繁博张宇闫成亮胡亚京
Owner JIANGSU TIANSHENG PHARMA
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