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Respiratory adenovirus IGA antibody detection test strip and its detection method

An antibody detection and adenovirus technology, applied in the field of medical testing, can solve the problems of nausea or vomiting, patient discomfort, difficult diagnosis methods, etc., and achieve the effect of improving the promotion rate, avoiding discomfort, and quick results.

Active Publication Date: 2018-04-24
GUANGZHOU RHFAY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology requires professional technicians and specialized fluorescent PCR amplification instruments, and it is difficult for primary medical departments to carry out this diagnostic method
In addition, this technology needs to collect cells from the patient's throat, which is more likely to cause discomfort in the patient and cause retching or vomiting.

Method used

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  • Respiratory adenovirus IGA antibody detection test strip and its detection method
  • Respiratory adenovirus IGA antibody detection test strip and its detection method
  • Respiratory adenovirus IGA antibody detection test strip and its detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] [Example 1] Preparation of Respiratory Adenovirus IgA Antibody Diagnostic Reagent Strips

[0025] 1. Source of raw materials: Goat anti-human IgA antibody was purchased from Sigma Company, and human IgA antibody was purchased from Guangzhou Nuosi Biotechnology Co., Ltd. The sequence of the polypeptide fragment of respiratory adenovirus hexon protein antigen is: Met Leu Leu Gly Asn GlyArg Tyr Val Pro Phe His Ile Gln Val Pro Gln Lys Phe Ala Val Lys Asn LeuLeu Leu Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys Asp Val Asn MetVal Leu (Seq ID NO.1), a total of 46 amino acids, was entrusted to Shanghai Jier Biochemical Company to be responsible for the synthesis, with a purity greater than 98%, and was stored in a lyophilized form.

[0026] 2. Preparation of goat anti-human IgA antibody colloidal gold labeling pad

[0027] In the pH 6.8-7.0 solution, the goat anti-human IgA antibody is labeled with colloidal gold particles to form a goat anti-human IgA antibody colloida...

Embodiment 2

[0032] [Example 2] Preparation of sterile absorbent cotton

[0033] Purchase medical absorbent cotton balls with a diameter of 2cm, and bind them with cotton threads with a length of 8-10cm. Place the treated cotton balls in a pressure cooker under the pressure condition of 121° C. for 30 minutes, place them in an oven for drying at a temperature of 100° C. for 4 hours, and vacuum-seal the dried cotton balls in an aluminum foil bag.

Embodiment 3

[0034] [Example 3] Saliva sample collection

[0035] In the present invention, the aseptic absorbent cotton is placed under the tongue of the child, the cotton thread is exposed outside the mouth, and the mouth is held for 3-5 minutes. as attached figure 2 , put the cotton ball 22 into the syringe 21, then install the syringe push rod and push it gently to obtain a saliva sample 23, which is stored in a 2ml small tube.

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Abstract

A respiratory adenovirus IgA antibody detection test strip, comprising a sample pad, a marker pad, a nitrocellulose membrane, an absorbent paper, and a backboard, characterized in that the cellulose membrane includes spray-coated respiratory adenovirus hexon protein The detection line of the antigen polypeptide and the control line of the human IgA antibody. The invention collects saliva samples through sterile absorbent cotton, squeezes out the saliva samples with a syringe, and detects the IgA antibody in the saliva through rapid chromatography paper strips of respiratory adenovirus IgA antibodies, thereby realizing the purpose of rapidly diagnosing patients infected with respiratory adenoviruses. The method is simple in operation and rapid in results, and is suitable for rapid diagnosis of respiratory adenovirus infection in medical institutions at all levels. The collection of specimens is non-invasive, avoiding the discomfort caused by traditional blood collection and nasopharyngeal swab collection to patients, and also improving the work efficiency of medical staff and improving the clinical promotion rate of respiratory adenovirus diagnosis.

Description

technical field [0001] The invention belongs to the field of medical testing, and specifically discloses a test strip for detecting respiratory adenovirus IgA antibody and a detection method thereof. Background technique [0002] Adenovirus (adenovirus) particles have a diameter of 60-90nm, no envelope, and 20-hedron stereosymmetry. The capsid is composed of 252 grams of particles, of which 240 shell particles are hexons (Hexon), with group-specific α antigens. Adenovirus has 6 species and 52 different serotypes, about 1 / 3 of which can cause acute respiratory infection, epidemic keratitis, acute hemorrhagic conjunctivitis, pharyngeal conjunctivitis, acute pharyngitis, cystitis, infant death diseases such as pneumonia. In my country, about 5% of acute respiratory diseases in children under the age of 5 are caused by adenoviruses. Pneumonia caused by adenoviruses can easily lead to serious illnesses. Adenovirus pneumonia can not only cause heart failure and respiratory failur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 李有生刘轶锴
Owner GUANGZHOU RHFAY BIOTECH CO LTD