Osmunda mildei fertile spore cultivation and reproduction method
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A technology for spores and spores of Radix vulgaris is applied in the field of fertile spore culture and reproduction of Radix Radix.
Inactive Publication Date: 2016-12-07
SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
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[0005] The object of the present invention is to overcome the problem of difficult spore propagation of Osmanthus edulis plant in the prior art, and provide a method for fertile spore germination and tissue culture rapid propagation suitable for heterozygous Osmanthus osmanthus
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Embodiment 1
[0021] This embodiment includes the following steps:
[0022] Disinfection treatment of S1 spores:
[0023] From March to May, the sporophytes of Osmanthus fragrans are fully unfolded, and when the sporangia begin to change from dark green to light green, pick off the sporophyll leaves, immediately wash away the floating dust on the surface with clean water, and collect the spores after the water on the surface of the sporophyll leaves is dried or, place the spore leaves in a petri dish covered with sulfuric acid paper, dry them in a desiccator and collect the spore powder. Then put it into a small test tube on the ultra-clean workbench, soak it in sterilized water for 5-10 minutes, centrifuge it with a centrifuge at 3500 rpm, pour off the supernatant, and add 0.1% HgCl 2 Disinfect the solution for 2-6 minutes, rinse with sterilized water for 5-6 times, and finally add an appropriate amount of sterilized water and mix to make a suspension.
[0024] In this step, it is best t...
Embodiment 2
[0048] S1: From March to May, the spore leaves of Osmanthus fruticosa are fully unfolded, and when the sporangia begin to change from dark green to light green, the spore leaves are removed, and the floating dust on the surface is immediately washed away with clean water, and each spore leaflet is removed. Clean the bench with 0.1% HgCl 2 The solution was sterilized for 2-6 minutes, rinsed with sterilized water for 5-6 times, the surface moisture was blotted with sterilized filter paper, and cut into small pieces containing 2-3 sporangia.
[0049] In this step, it is best to sterilize for 5 minutes, and for simple multiplication culture not for the purpose of observing gametophyte development, it is convenient to directly sterilize with sporophylls.
[0050] S2 prothalloid acquisition:
[0051] Inoculate the sporangia fragments sterilized in step S1 on MS basic medium, add hormone 6-benzylpurine 0.5-1.5㎎ / L, naphthaleneacetic acid 0.01-0.1㎎ / L, at a temperature of (25±2)℃, Und...
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Abstract
The invention provides an osmunda mildei fertile spore cultivation and reproduction method. The osmunda mildei fertile spore cultivation and reproduction method includes inoculating an MS basic culture medium with sterilized sporangium chips or spore powder, adding 0.5-1.5mg / L of 6-benzyl adenine and 0.01-0.1mg / L of naphthylacetic acid for cultivation, and allowing spores to grow into prothalli gradually from filaments and plates; transferring the prothalli into 1 / 2MS and 1 / 4MS culture media for cultivation, and after the volume of the prothalli is obviously increased, transferring the prothalli into the same solid culture medium for cultivation to form spore seedlings; inoculating a culture medium containing 0.5-1.0mg / L of indolebutyric acid with the spore seedlings for cultivation, continuing cultivation in an original bottle for around one month after radicles are formed 20 days later, and transferring the radicles to a pot for cultivation. By the osmunda mildei fertile spore cultivation and reproduction method, the problem of difficulty in reproduction of osmunda mildei is solved, osmunda mildei seedlings can be reproduced quickly in a short time, and rare resources of the osmunda mildei are protected effectively.
Description
technical field [0001] The invention belongs to the technical field of fern tissue culture, and in particular relates to a fertile spore culture and a propagation method thereof. Background technique [0002] Ferns play an important role as species resources and maintain ecological balance; secondly, they are also of great scientific value in the study of the origin, evolution and classification of plants; thirdly, they also have medicinal, edible, ornamental and other aspects the use of. Therefore, tissue culture technology has been widely used in the research and production of ferns. At present, in the tissue culture research of ferns, the explants can use underground stem segments or shoot tips, young leaves or leaf primordia, spores, etc. Usually, the subterranean stem segments or stem tips, young leaves or leaf primordia of ferns are covered with scales, which makes it difficult to sterilize. Moreover, the number of individuals of rare species is very small, and the s...
Claims
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Application Information
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