A kind of food-grade bamboo leaf extract nano liposome and its preparation method and application

A technology of bamboo leaf extract and nano-liposome, which is applied in the fields of application, food science, food preservation, etc., and achieves the effects of high yield, easy large-scale production, good economic benefits and application prospects

Active Publication Date: 2019-09-03
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no fresh-keeping application of bamboo leaf extract nanoliposomes for surimi, beverages and other products

Method used

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  • A kind of food-grade bamboo leaf extract nano liposome and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Take dried bamboo leaves, crush them, pass through 40 meshes, add bamboo leaves and 50-75% ethanol solution respectively at a ratio of 10Kg: 50L to 10Kg: 200L with a material-liquid ratio, and extract 20% with the aid of ultrasonic waves at a temperature of 40-70 degrees. -100 minutes to obtain the extract containing bamboo leaves.

[0031] 2. Adsorbed by macroporous adsorption resin with polystyrene skeleton, and eluted with 4 times column volume of 75% ethanol. The eluate is separated by ultrafiltration membrane and concentrated under reduced pressure to obtain 25-50 L of bamboo leaf extract concentrate.

[0032] 3. 20 parts of bamboo leaf extract concentrate, 30 parts of soybean lecithin, 40 parts of egg yolk lecithin, 5 parts of vitamin E, 2 parts of cholesterol, and 3 parts of vitamin C, mixed and fully dissolved in ethanol solution to obtain lipid solution, at temperature Under the condition of 50 degrees Celsius, the organic solvent is removed under reduced p...

Embodiment 2

[0035] 1. Select dry bamboo leaves, crush them, pass through 40 meshes, add bamboo leaves and 30-85% ethanol solution at a ratio of 1Kg: 5L to 1Kg: 20L with a material-to-liquid ratio, and extract 50% with the aid of ultrasonic waves at a temperature of 40-70 degrees. Minutes, obtain containing bamboo leaf extract.

[0036] 2. Adsorbed by macroporous adsorption resin with polystyrene skeleton, and eluted with 75% ethanol with 5 times column volume. The eluate is separated by ultrafiltration membrane and concentrated under reduced pressure to obtain 2-5L of bamboo leaf extract concentrate.

[0037] 3. 10 parts of bamboo leaf extract concentrate, 40 parts of soybean lecithin, 45 parts of egg yolk lecithin, 2 parts of vitamin E, 1 part of cholesterol, and 2 parts of vitamin C are mixed and fully dissolved in ethanol solution to obtain a lipid solution. Under the condition of 40 degrees Celsius, use a rotary evaporator to remove the organic solvent under reduced pressure, and the...

Embodiment 3

[0040]1. Select dry bamboo leaves, crush them, pass through 30 meshes, add bamboo leaves and 30-75% ethanol solution at a ratio of 5Kg: 20L to 10Kg: 50L with a material-to-liquid ratio, and use ultrasonic-assisted extraction for 60 Minutes, obtain containing bamboo leaf extract.

[0041] 2. Adsorbed by macroporous adsorption resin with polystyrene skeleton, and eluted with 75% ethanol with 5 times column volume. The eluate is separated by an ultrafiltration membrane and concentrated under reduced pressure to obtain 2-8 L of bamboo leaf extract concentrate.

[0042] 3. 15 parts of bamboo leaf extract concentrate, 50 parts of soybean lecithin, 30 parts of egg yolk lecithin, 2 parts of vitamin E, 1 part of cholesterol, and 3 parts of vitamin C, mixed and fully dissolved in ethanol solution to obtain lipid solution, at temperature Under the condition of 45 degrees Celsius, use a rotary evaporator to remove the organic solvent under reduced pressure, and the temperature is 30-50 d...

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Abstract

The present invention relates to food-grade bamboo leaf extract nano-liposomes. The food-grade bamboo leaf extract nano-liposomes are prepared from, 5-20 parts of bamboo leaf extract concentrated bamboo liquid, 20-60 parts of soybean lecithin, 30-50 parts of egg yolk lecithin, 1-5 parts of vitamin E, 1-6 parts of cholesterol, and 1-10 parts of vitamin C by ultrasonic hydration, high pressure homogenization and reverse evaporation-thin film hydration-homogenization. The encapsulation ratio of the food-grade bamboo leaf extract nano-liposomes is 40-70%. The food-grade bamboo leaf extract nano-liposomes contain 0.01-50% of the bamboo leaf extract, have particle sizes of 300-500 [mu]m, are uniform and stable, can effectively replace preservatives to be used for food preservation, and extend shelf life.

Description

technical field [0001] The present invention relates to a kind of food-grade bamboo leaf extract nano liposome and its preparation method and application, particularly to a kind of using natural bamboo leaf extract, soybean lecithin, egg yolk lecithin, vitamin E, cholesterol, vitamin C, through Ultrasonic hydration, high-pressure homogenization, reverse evaporation method-thin film hydration-homogenization is prepared, which can effectively replace preservatives for food preservation and extend shelf life. [0002] technical background [0003] Bamboo is a perennial gramineous plant. China is one of the big countries with the most abundant bamboo plant resources. There are more than 500 species of bamboo plants in 48 genera. Bamboo stems and leaves contain various flavonoids, phenolic compounds, proteins and sugars, tea polyphenols, exogenous antioxidants, amino acids and other valuable components. Flavonoids in bamboo leaves mainly exist in the form of glycosides, and there...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L3/3472A23L3/3517A23L3/3544A23L3/3553A23L2/52A23L2/44A23P10/43
Inventor 陈丽春邓勇毛建卫沙如意
Owner ZHEJIANG GONGSHANG UNIVERSITY
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