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Anticancer composition as well as preparation and application thereof

A composition and technology for anticancer drugs, applied in the field of preparation and application of anticancer drugs, can solve problems such as metastasis, drug tolerance, treatment failure and the like

Inactive Publication Date: 2016-12-07
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapy can produce a certain curative effect in the initial stage of treatment, but as time goes on, drug resistance often occurs, tumor recurrence or metastasis, leading to treatment failure

Method used

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  • Anticancer composition as well as preparation and application thereof
  • Anticancer composition as well as preparation and application thereof
  • Anticancer composition as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 In vitro cytotoxicity test of docetaxel and dexamethasone in combination

[0058] Use complete medium to culture KB (purchased from National Nano Center), KBv (purchased from National Nano Center), PC-3 (Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences), 4T1 (ATCC, USA), 3T3 (Basic Medical Research Institute, Chinese Academy of Medical Sciences) Institute), Hela (Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences), MDA-MB-231 (Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences), B16-F10 cells (Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences), when the cells grow to the logarithmic phase Inoculate 180 μl per well of a 96-well plate, approximately 5,000 cells; culture in an incubator for 24 hours to allow the cells to adhere to the wall, and each cell is given dexamethasone, docetaxel, or dexamethasone and docetaxel at the same time The final concentration of dexamethaso...

Embodiment 2

[0060] Example 2 In vitro cytotoxicity test of docetaxel and dexamethasone in combination

[0061] Use complete medium to culture KB, KBv, PC-3, 4T1 cells. When the cells grow to the logarithmic phase, inoculate 180 μl per well of a 96-well plate, about 5000 cells; cultivate in an incubator for 24 hours to make the cells adhere to the wall. The cells were treated with dexamethasone, docetaxel, and dexamethasone for 1 hour before docetaxel; the final concentrations of dexamethasone administration groups were 0.1, 1, 5, 10, 20, 50, 80 100 μg / ml, docetaxel administration concentration is 5 μg / ml, cultivate in the incubator for 48 hours, discard the medium. Then add 100 μl of complete medium containing 5% CCK-8 solution; after incubation for 2 hours, use a microplate reader to detect the absorbance of each group at 450 nm.

[0062] From the results of cytotoxicity, it can be seen that when the concentration of docetaxel is above 10 μg / ml, it has a certain inhibitory effect on the...

Embodiment 3

[0063] Example 3 In vitro cytotoxicity test of paclitaxel combined with dexamethasone

[0064] The specific experimental method is the same as in Example 1, and paclitaxel is used instead of docetaxel.

[0065] From the results of cytotoxicity, it can be seen that when the concentration of dexamethasone is above 10 μg / ml, it has a certain inhibitory effect on the above cell lines; compared with paclitaxel alone, the inhibitory effect of dexamethasone and paclitaxel group is increased by 10- About 40%, it has a better tumor suppression effect.

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Abstract

The invention discloses an anticancer composition as well as a preparation and application thereof and belongs to the field of preparation and application of anticancer drugs. The invention firstly discloses the anticancer composition. The anticancer composition is prepared from dexamethasone with effective dose in treatment and an anticancer drug. Compared with a mode of independently using the anticancer drug, a mode of jointly using the dexamethasone and the anticancer drug, disclosed by the invention, has a better tumor inhibiting effect. The invention further discloses an anticancer preparation. The anticancer preparation is prepared from the anticancer composition with the effective dose in treatment and pharmaceutically-acceptable accessories. Founded by the invention, when the anticancer preparation is prepared into a nano preparation for application, the dexamethasone can be released into a tumor microenvironment by using a targeting action and a slow release effect, related components of the tumor microenvironment is improved, the accumulation of the anticancer drug at a tumor part is further improved, the growth and the transfer of tumors are significantly inhibited, tumor cells are killed and an anticancer effect is enhanced; the inhibition rate of the tumors in a body reaches 97 percent, toxic or side effects of the anticancer drug can be relieved, and the treatment cost and the risk of the cancer are reduced.

Description

technical field [0001] The present invention relates to an anticancer composition, in particular to an anticancer composition made of dexamethasone and one or more anticancer drugs. The present invention also relates to the application of the anticancer composition in the preparation of anticancer preparations, which belongs to Preparation and application fields of anticancer drugs. Background technique [0002] Cancer is one of the main chronic diseases affecting the health of Chinese residents, and has become the number one cause of death among Chinese residents. According to the epidemiological survey of cancer in China, the proportion of cancer among the causes of death in China has increased from 10.13% in the 1970s to 22.32% in the past 30 years, and the death rate has increased from 73.99 / 100,000 to 135.88 / 100,000. Moreover, with the aggravation of factors such as population aging, the incidence and mortality of tumors are still on the rise. According to the forecas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61K31/573A61K31/337A61K9/127A61K9/107A61P35/00
CPCA61K9/1075A61K9/127A61K31/337A61K31/573A61K45/06A61K47/24A61K2300/00
Inventor 齐宪荣苏海涛
Owner PEKING UNIV
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