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Phospholipid-containing microemulsion, its preparation method, and its application in the study of interfacial water properties

A microemulsion and phospholipid technology, which is applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of low water molecule content, inability to form a phospholipid film, and reducing the water signal-to-noise ratio and resolution of THz-TDS detection of phospholipid film interface. , to achieve the effect of reducing strong absorption, increasing sensitivity, and simple preparation method

Active Publication Date: 2018-12-04
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the phospholipid membrane interface water in the prior art in the preparation process, the water molecule content is less, can not form the phospholipid membrane closer to the physiological state, or in the process of forming the phospholipid membrane of the physiological state, The content of water molecules is relatively large, so that the THz pulse signal intensity is greatly affected by the absorption of water molecules, which reduces the defect of signal-to-noise ratio and resolution of THz-TDS detection of phospholipid membrane interface water, and provides a phospholipid-containing microemulsion and Preparation method, application in the study of interfacial water properties

Method used

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  • Phospholipid-containing microemulsion, its preparation method, and its application in the study of interfacial water properties
  • Phospholipid-containing microemulsion, its preparation method, and its application in the study of interfacial water properties
  • Phospholipid-containing microemulsion, its preparation method, and its application in the study of interfacial water properties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The preparation method of the microemulsion containing phospholipid, comprises the steps:

[0057] (1) Take 0.37 μmol of three kinds of raw material phospholipids (DOPC, DOPE or DOPG) and place them at the bottom of three glass test tubes respectively. The three kinds of raw material phospholipids are all dissolved in chloroform, and the concentration is 25mg / mL, and three parts of chloroform containing phospholipids are obtained. solution;

[0058] (2) Suspend the ventilation needles connected to the nitrogen tank to 4 cm above the phospholipid-containing chloroform solution, inject nitrogen slowly, maintain the air velocity at two atmospheres, and maintain the operating temperature at room temperature 25°C for 45 minutes. After passing nitrogen gas, three parts of gelatinous white phospholipid films (referred to as phospholipid film B) were formed at the bottom of three glass test tubes respectively. 1 , Phospholipid film B 2 , Phospholipid film B 3 );

[0059] In...

Embodiment 2

[0079] The preparation method of the sample to be tested in this embodiment is the same as that in Example 1. This example can be used to explore the influence of sodium chloride salt ions on the interfacial water of phospholipid membranes.

[0080] Test Conditions:

[0081] Sample: DOPC, DOPC / NaCl

[0082] Phospholipid Solvent: Hexadecane

[0083] Phospholipid hydration agent: pure water (18.2Ω)

[0084] NaCl concentration: 100mM, 500mM

[0085] Among them, NaCl was purchased from Sinopharm Chemical Reagent Co., Ltd. In the microemulsion containing phospholipids, the molar ratio of water to phospholipids is 150:1; the volume ratio of hexadecane and ultrapure water is 90:10.

[0086] Figure 4 It is the absorption coefficient diagram of the DOPC phospholipid microemulsion containing different concentrations of sodium chloride on the terahertz band. Depend on Figure 4 It can be seen that the absorption coefficient of the microemulsion increases with the increase of the...

Embodiment 3

[0088] The preparation method of the sample to be tested in this embodiment is the same as that in Example 1. This example can be used to explore the hydration degree of the phospholipid membrane interface in the state of complex phospholipid components.

[0089] In Example 1, a single phospholipid is replaced by a complex of two or more phospholipids. The basis for selecting multiple phospholipid components is whether phase separation occurs under experimental conditions. Taking DOPC / DPPC and DOPC / DOPE as examples, DOPC / DPPC DPPC has obvious phase separation at room temperature; the two phospholipids in the DOPC / DOPE composite system are liquid at room temperature, and the PE molecules with amino groups in the head will be interspersed between the PC heads to reduce the dipole between the PC heads. Electrostatic repulsion. Based on this, the effect of phase separation state and rearrangement combination of phospholipid molecular heads on the hydration degree of phospholipid ...

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Abstract

The invention discloses microemulsion containing phospholipid, a preparation method thereof and application thereof in study of interface water properties. The preparation method comprises the following steps: carrying out vortex oscillation on mixed solution of a phospholipid thin film, cetane and ultrapure water, and carrying out ultrasonic treatment, so that the microemulsion is prepared, wherein the phospholipid in the phospholipid thin film is one or more of DOPC, DOPE and DOPG; in the microemulsion, molar ratio of water to the phospholipid is (100-150):1, and volume ratio of cetane to the ultrapure water is (90-95):(5-10). The preparation method of the microemulsion is simple, the obtained microemulsion is not doped with other cosolvent, stability is good, a phospholipid membrane can be in a completely hydrated state, and water molecule content in a system can be as low as possible, so that strong absorption of the water molecules in the system to terahertz light wave is reduced, sensitivity of THz-TDS (total dissolved solids) detection on interfacial water of the phospholipid membrane is improved, and influence on signal-to-noise ratio and resolution ratio during detection is small.

Description

technical field [0001] The invention relates to a phospholipid-containing microemulsion, its preparation method and its application in researching the properties of interface water. Background technique [0002] Phospholipid membrane interface water (hydration water on lipid membrane surface) refers to the multi-layered water molecules in the range of about 1nm on the surface of the phospholipid membrane, and the dielectric response function is obviously interfered by the solvation of phospholipid molecules. These interfacial water molecules play a crucial role in the fluidity and function of the membrane itself, as well as the repulsive force related to the fusion between the membranes; the interaction between the membrane and the interfacial water molecules affects the change of the interface dipole potential, thereby regulating the ion, Transport of biomolecules, drugs, etc. between membranes. Therefore, it is particularly important to study the physical and chemical pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/3586
CPCG01N21/3586
Inventor 潘亚涛吕军鸿
Owner SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI