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Developing agent for thin-layer identification method for traditional Chinese medicine material radix et rhizoma rhei and thin-layer identification method

A technology of thin-layer identification and developing agent, which is applied in the direction of material separation, material analysis, measuring devices, etc., can solve the problems of long time and achieve the effects of improving efficiency, simplifying configuration steps, and saving time

Inactive Publication Date: 2016-12-07
成都维恒医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this method takes too long

Method used

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  • Developing agent for thin-layer identification method for traditional Chinese medicine material radix et rhizoma rhei and thin-layer identification method
  • Developing agent for thin-layer identification method for traditional Chinese medicine material radix et rhizoma rhei and thin-layer identification method
  • Developing agent for thin-layer identification method for traditional Chinese medicine material radix et rhizoma rhei and thin-layer identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Preparation of the test solution: 0.75g Rheum palmatum (purchased from Tong Ren Tang) and 10 mL of 85% ethanol were mixed, the first ultrasonic treatment was performed twice, each time for 15 minutes, followed by filtration, and then the filtrate was collected twice and mixed The first filtrate is obtained, and the first filtrate is evaporated to dryness to obtain the first extract. After the first extract and 10 mL of water were mixed, the second ultrasonic treatment was carried out for 15 minutes, and then dichloromethane was added for extraction twice, and the amount of dichloromethane added each time was 15 mL. The dichloromethane liquid was collected twice and evaporated to dryness. 1mL of methanol was dissolved to obtain the rhubarb palmatum medicinal material solution.

[0054] Preparation of the reference drug solution: Mix 0.75 g of Rheum palmatum reference drug with 10 mL of 85% ethanol, perform the first ultrasonic treatment twice, each time for 15 minutes, t...

Embodiment 2

[0059] The preparation of need testing solution: 1.5g rhubarb palmatum and 20mL of 85% ethanol are mixed, carry out the first ultrasonic treatment twice, each 30 minutes, then filter, then collect twice filtrate and mix to obtain the first filtrate, The first filtrate was evaporated to dryness to obtain the first extract. Then the first extract and 20mL of water were mixed and subjected to the second ultrasonic treatment for 30min, then added dichloromethane for extraction twice, each time the dichloromethane added was 25mL, the dichloromethane liquid was collected twice and evaporated to dryness. 1mL of methanol was dissolved to obtain the rhubarb palmatum medicinal material solution.

[0060] Preparation of the reference medicinal material solution: 1.5 g of Rheum palmatum reference medicinal material and 20 mL of 85% ethanol were mixed, the first ultrasonic treatment was performed twice, each time for 30 minutes, followed by filtration, and then the filtrates were collected...

Embodiment 3

[0065] Preparation of the test solution: 0.75g Tangut rhubarb reference medicinal material and 10mL of 85% ethanol were mixed, the first ultrasonic treatment was performed twice, each time for 15 minutes, and then filtered, and then the filtrate was collected twice and mixed to obtain the first filtrate, the first filtrate was evaporated to dryness to obtain the first extract. Then the first extract and 10mL of water were mixed and subjected to the second ultrasonic treatment for 15min, then added dichloromethane for extraction twice, and the dichloromethane added each time was 25mL, and the dichloromethane liquid was collected twice and evaporated to dryness. 1mL of methanol was dissolved to obtain the Tangut rhubarb reference drug solution.

[0066] Preparation of the reference solution: take chrysophanol and emodin, add methanol respectively to prepare a chrysophanol medicinal solution with a mass concentration of 1mg / 1mL and an emodin medicinal solution with a mass concent...

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Abstract

The invention discloses a developing agent for a thin-layer identification method for the traditional Chinese medicine material radix et rhizoma rhei and the thin-layer identification method, and relates to the field of traditional Chinese medicine material identification. The thin-layer identification method for the traditional Chinese medicine material radix et rhizoma rhei includes the step of developing, wherein a medicine material solution is added to a silica gel thin layer plate in a dotted mode and is developed with the developing agent, the developing agent is a mixture of petroleum ether, glacial acetic acid and a first solvent, and the first solvent is any one of ethyl acetate, chloroform and dichloromethane. The developing agent adopted in the method is directly prepared and used, it is unnecessary to take an upper-layer solution after standing, and preparation steps of the developing agent are simplified; meanwhile, time is saved, and efficiency is improved. In a preferred embodiment, active ingredients in the medicine material radix et rhizoma rhei are extracted with the ultrasonic method, experimental steps are simplified, preparation time is shortened, and identification efficiency is improved; the preparation steps of the developing agent are simple, and the developing effect is good; a solvent for preparing the sample solution is optimized. The identification method is simple and easy to implement, and the traditional Chinese medicine material radix et rhizoma rhei can be rapidly and accurately identified with the identification method.

Description

technical field [0001] The invention relates to the field of identification of Chinese medicinal materials, in particular to a developing agent used in a thin-layer identification method of rhubarb, a Chinese medicinal material, and a thin-layer identification method. Background technique [0002] Rhubarb has the effects of purging and attacking stagnation, clearing heat and purging fire, cooling blood and detoxifying, expelling blood stasis and dredging menstrual flow, promoting dampness and reducing jaundice; it is often used for constipation due to excess heat stagnation, vomiting of blood heat, red eyes and pharynx, carbuncle and furuncle, Intestinal abscess, abdominal pain, blood stasis, amenorrhea, postpartum stasis, bruises, damp-heat dysentery, jaundice and red urine, stranguria, edema; sources of resources are Rheum pallmatum L., medicinal rhubarb (Rheumoffcinale Baill.) , dry roots and rhizomes of Rheum tanguticum Maxim.ex Balf. [0003] At present, the TLC identi...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94
Inventor 刘柳沈芃刘涛周锡建卢福曹晓红杜全胜陈洪
Owner 成都维恒医药科技有限公司