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Preparing method and application of molasses colloid composite alginate immobilizing carrier

An immobilized carrier, alginate technology, applied in the direction of being fixed on or in an inorganic carrier, immobilized on/in an organic carrier, etc., can solve problems such as insufficient utilization of molasses raw materials, and achieve improved physical strength. Effect

Active Publication Date: 2016-12-14
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Molasses contains 10%-20% colloidal substances, and these colloidal substances are removed during the molasses pretreatment process because they can inhibit the activity of microorganisms and enzymes, so that the molasses raw materials cannot be fully utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 This example illustrates the preparation method of molasses colloid composite alginate immobilization carrier

[0034] 1. Colloid extraction

[0035] 1) Mix molasses and water in equal volumes to obtain a raw material liquid.

[0036] 2) Petroleum ether was used as the extractant, molasses solution was used as the raw material, and the volume ratio of extractant and raw material was 1:1, 40°C, 200 rpm and mixed for 10 min. solution, colloidal solid in the middle layer and aqueous solution in the lower layer.

[0037] 3) Add absolute ethanol to the colloidal solid in the middle layer, the colloidal solid precipitates, and the precipitate is collected, which is the extracted colloid.

[0038] 2. Freeze drying of colloids

[0039] Vacuum freeze-drying conditions were -50°C for 2 h.

[0040] 3. Colloidal esterification

[0041] 1) Mix concentrated sulfuric acid and n-propanol, wherein the volume ratio of concentrated sulfuric acid and n-propanol is 1:1;

[0...

Embodiment 2

[0054] Example 2 This example illustrates the preparation method of molasses colloid composite alginate immobilization carrier

[0055] 1. Colloid extraction

[0056] 1) Mix molasses and water in equal volumes to obtain a raw material liquid.

[0057] 2) Petroleum ether was used as the extractant, molasses solution was used as the raw material, and the volume ratio of the extractant and the raw material was 1:1, 40°C, 200 rpm and mixed for 20 min. solution, colloidal solid in the middle layer and aqueous solution in the lower layer. .

[0058] 3) Add absolute ethanol to the colloidal solid in the middle layer, the colloidal solid precipitates, and the precipitate is collected, which is the extracted colloid.

[0059] 2. Freeze drying of colloids

[0060] Vacuum freeze-drying conditions were -50 °C for 4 h.

[0061] 3. Colloidal esterification

[0062] 1) Mix concentrated sulfuric acid and n-propanol, wherein the volume ratio of concentrated sulfuric acid and n-propanol is ...

Embodiment 3

[0075] Example 3 This example illustrates the preparation method of molasses colloid composite alginate immobilization carrier

[0076] 1. Colloid extraction

[0077] 1) Mix molasses and water in equal volumes to obtain a raw material liquid.

[0078] 2) Using petroleum ether as the extractant and molasses solution as the raw material, mix for 15 min at the volume ratio of the extractant and the raw material at 1:1, 40°C, and 200 rpm. solution, colloidal solid in the middle layer and aqueous solution in the lower layer. .

[0079] 3) Add absolute ethanol to the colloidal solid in the middle layer, the colloidal solid precipitates, and the precipitate is collected, which is the extracted colloid.

[0080] 2. Freeze drying of colloids

[0081] Vacuum freeze-drying conditions were -50 °C for 4 h.

[0082] 3. Colloidal esterification

[0083] 1) Mix concentrated sulfuric acid and n-propanol, wherein the volume ratio of concentrated sulfuric acid and n-propanol is 1:2;

[00...

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PUM

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Abstract

The invention discloses a preparing method of a molasses colloid composite alginate immobilizing carrier and application of the carrier in immobilizing microorganism cells and enzymes. According to the preparing method, the molasses colloid composite alginate immobilizing carrier is obtained through the steps of colloid extraction, colloid vacuum freeze drying, colloid esterification, esterified colloid vacuum freeze drying, esterified colloid dry powder smashing, immobilizing carrier preparing and the like. The physical strength of the molasses colloid composite alginate immobilizing carrier is improved by 30-60%, and the colloid substance abandoned in the molasses utilization process is utilized. By implementing the preparing method, comprehensive utilization of inferior biomass molasses is promoted, and a technical base is provided for molasses biorefining.

Description

technical field [0001] The invention relates to a method in the technical field of biochemical industry, in particular to a method for strengthening an alginate immobilized carrier, which can be used in a biocatalysis process. Background technique [0002] The stress resistance of immobilized microbial cells and immobilized enzymes is stronger than that of free cells and free enzymes. At the same time, immobilized cells and immobilized enzymes can achieve the purposes of repeated use, continuous operation, cost saving, and simplified product separation and purification processes. [0003] The commonly used microbial cell and enzyme immobilization methods include binding method, cross-linking method, embedding method and so on. Among them, sodium alginate-CaCl 2 Embedding is a common method of immobilizing microorganisms and immobilizing enzymes, which has the advantages of simple operation, low cost, high activity, and no toxicity to microbial cells and enzymes. However, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/02
CPCC12N11/02C12N11/14
Inventor 陈可泉何珣花加伟李梅欧阳平凯
Owner NANJING UNIV OF TECH
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