DC-targeting Porcine Reproductive and Respiratory Syndrome PLGA nano adjuvant inactivated vaccine, and preparation method thereof

A technology of porcine blue ear disease and inactivated vaccines, applied in the field of biological vaccines, can solve problems such as unsatisfactory effects and slow immune responses, and achieve the effects of strengthening cellular immune responses, prolonging half-life, and delaying release time

Pending Publication Date: 2016-12-21
DALIAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The occurrence of porcine blue-ear disease has caused great economic losses to the breeding industry in my country. At present, there is no effective drug that can completely cure it. Immunization and prevention of sows and piglets is the most economical and effective way to control porcine blue-ear disease. Current inactivated PRRS vaccines and live attenuated vaccines have slow immune responses and unsatisfactory effects

Method used

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  • DC-targeting Porcine Reproductive and Respiratory Syndrome PLGA nano adjuvant inactivated vaccine, and preparation method thereof
  • DC-targeting Porcine Reproductive and Respiratory Syndrome PLGA nano adjuvant inactivated vaccine, and preparation method thereof
  • DC-targeting Porcine Reproductive and Respiratory Syndrome PLGA nano adjuvant inactivated vaccine, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of PLGA Nano-Adjuvanted Vaccines

[0037] (1) Phase W2: Prepare 0.25wt% PVA (polyvinyl alcohol) solution with 30mL distilled water, put it into the rotor, seal it with a parafilm, put it in a constant temperature heating magnetic stirrer, add water to the constant temperature heating magnetic stirrer to cover the iron ring , adjust the appropriate speed, and adjust the voltage to the maximum. After the water boils, adjust the voltage to gradually decrease until the PVA is completely dissolved and then taken out.

[0038] (2) Phase O: Take a 5mL centrifuge tube, add 2mL of dichloromethane, weigh 0.01wt% PLGA (polylactic acid glycolic acid) into the dichloromethane, shake on a vortex shaker until PLGA is completely dissolved.

[0039] (3) Phase W1: Weigh 0.01wt% Poly(I:C) (polyinosinic acid-polycytidylic acid) into a 1mL centrifuge tube, and mix with TCID 50 =5×10 5 PRRSV (highly pathogenic porcine reproductive and respiratory syndrome inactivated virus) is ...

Embodiment 2

[0051] Preparation of PLGA Nano-Adjuvanted Vaccines

[0052] (1) Phase W2: Prepare 0.75wt% PVA (polyvinyl alcohol) solution with 30mL distilled water, put it into the rotor, seal it with a parafilm, put it in a constant temperature heating magnetic stirrer, add water to the constant temperature heating magnetic stirrer to cover the iron ring , adjust the appropriate speed, and adjust the voltage to the maximum. After the water boils, adjust the voltage to gradually decrease until the PVA is completely dissolved and then taken out.

[0053] (2) Phase O: Take a 5mL centrifuge tube, add 2mL of dichloromethane, weigh 0.5wt% PLGA (polylactic acid glycolic acid) into the dichloromethane, shake on a vortex shaker until PLGA is completely dissolved.

[0054] (3) Phase W1: Weigh 0.1wt% Poly(I:C) (polyinosinic acid-polycytidylic acid) into a 1mL centrifuge tube, and mix with TCID 50 =5×10 6 PRRSV (highly pathogenic porcine reproductive and respiratory syndrome inactivated virus) is mixe...

Embodiment 3

[0066] Preparation of PLGA Nano-Adjuvanted Vaccines

[0067] (1) Phase W2: Prepare 1.25wt% PVA (polyvinyl alcohol) solution with 30mL distilled water, put it into the rotor, seal it with a parafilm, put it in a constant temperature heating magnetic stirrer, add water to the constant temperature heating magnetic stirrer to cover the iron ring , adjust the appropriate speed, and adjust the voltage to the maximum. After the water boils, adjust the voltage to gradually decrease until the PVA is completely dissolved and then taken out.

[0068] (2) Phase O: take a 5mL centrifuge tube, add 2mL of dichloromethane, weigh 5wt% PLGA (polylactic acid glycolic acid) into the dichloromethane, shake on a vortex shaker until PLGA is completely dissolved.

[0069] (3) Phase W1: Weigh 5wt% Poly(I:C) (polyinosinic acid-polycytidylic acid) into a 1mL centrifuge tube, mix with TCID 50 =5×10 7 PRRSV (highly pathogenic porcine reproductive and respiratory syndrome inactivated virus) is mixed, the...

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Abstract

The invention belongs to the technical field of biological vaccines, and more specifically relates to a DC-targeting Porcine Reproductive and Respiratory Syndrome PLGA nano adjuvant inactivated vaccine, and a preparation method thereof. One portion of the DC-targeting Porcine Reproductive and Respiratory Syndrome PLGA nano adjuvant inactivated vaccine used for 10 pigs comprises 5*10<5> to 5*10<7> of PRRSV TCID<50>, 1 to 20wt% of mannose, 0.01 to 5wt% of PLGA, and 0.01 to 5wt% of Poly(I:C). The preparation method mainly comprises two steps: S1, a PLGA nano adjuvant inactivated vaccine is prepared; and S2, a mannose-modified PLGA nano adjuvant vaccine is synthesized. According to the preparation method, mannose residue is used for modifying PLGA so as to establish a dendritic cell (DC), macrophage delivery system with mannose receptors; Poly(I:C) is added so as to improve immunity of immunized pigs and strengthen cellullar immunologic response; nontoxic degradable material PLGA is taken as a carrier to prepare the nano vaccine, so that virus release time is prolonged, drug toxicity is reduced, and drug half-life period is prolonged.

Description

technical field [0001] The invention relates to a vaccine and a preparation method thereof, in particular to a DC targeting porcine blue ear disease PLGA nano-adjuvant inactivated vaccine and a preparation method thereof, belonging to the technical field of biological vaccines. Background technique [0002] Pig PRRS, also known as porcine reproductive and respiratory syndrome (PRRS), is caused by PRRS virus (PRRSV), and is a highly contagious, highly fatal infectious disease characterized by reproductive disorders in sows and respiratory diseases in piglets. Since it was reported in the United States in 1987, it has spread to all major pig-raising countries in the world in recent years. It is one of the severe infectious diseases of animals notified by the International Veterinary Office (OIE), and it is a major infectious disease with high morbidity and mortality. . Since June 2006, an epidemic known as "high fever" or "unknown high fever" has broken out catastrophically i...

Claims

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Application Information

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IPC IPC(8): A61K39/12A61K39/39A61P31/14A61K31/787
CPCA61K39/12A61K31/787A61K39/39A61K2039/5252A61K2039/55511C12N2770/10034A61K2300/00
Inventor 王钦富刘欢胡亚萍王鹏张正一王美辰
Owner DALIAN UNIVERSITY
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