Application of nuclear FXR (Farnesoid X Receptor) in targeted therapy of liver cancer stem cells

A liver cancer stem cell and targeted therapy technology is applied in the application field of nuclear receptor FXR in the targeted therapy of liver cancer stem cells, which can solve the application difficulties and other problems, and achieve the solution of difficult regulation, reducing the ability of tumor sphere formation, and reducing tumorigenicity. sexual effect

Inactive Publication Date: 2016-12-21
HENAN UNIVERSITY
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Problems solved by technology

[0005] The isolation and identification of CSCs provides a theoretical basis for clinical targe

Method used

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  • Application of nuclear FXR (Farnesoid X Receptor) in targeted therapy of liver cancer stem cells
  • Application of nuclear FXR (Farnesoid X Receptor) in targeted therapy of liver cancer stem cells
  • Application of nuclear FXR (Farnesoid X Receptor) in targeted therapy of liver cancer stem cells

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Embodiment Construction

[0025] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0026] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0027] Human liver cancer cell line HepG2: purchased from Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences.

[0028] DMEM medium: purchased from Invitrogen, the catalog number is 11965-092.

[0029] DMEM / F12 medium: purchased from Invitrogen, the catalog number is 10565-018.

[0030] Serum: available from Gibco, catalog number 10099-141.

[0031] Pancreatin: available from Invitrogen, catalog number 25300-354.

[0032] Basic fibroblast growth factor (bFGF): purchased from Invitrogen, catalog number PHG0023.

[0033] Epidermal growth factor (EGF): purchased from Invitrogen, catalog number PHG0311.

[0034] Hepatic growth factor (HGF): purchased from PeproTech, catalog number 100-39.

[0035] b27: Avail...

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Abstract

The invention belongs to the field of biomedicines, and relates to application of a nuclear FXR (Farnesoid X Receptor) in targeted therapy of liver cancer stem cells. The nuclear FXR is used as a target for diagnosing or assisting in diagnosis of the liver cancer stem cells, and a gene medicine or a chemical medicine is designed to be applied to treatment of the liver cancer stem cells. According to the application of the nuclear FXR in the targeted therapy of the liver cancer stem cells, the gene medicine is an FXR-containing expression vector or interference vector; the chemical medicine contains a ligand for regulating the FXR and a ligand for regulating a target gene of the FXR. According to the application, by virtue of cell transfection and activation of the nuclear FXR, the tumor ball formation capacity of a hepatoma cell line can be obviously reduced, and the tumorigenicity of the liver cancer stem cells is reduced; a new molecular target is supplied to identification of the liver cancer stem cells and diagnosis and treatment of the liver cancer, so that the problem that a conventional molecular marker of tumor stem cells is hard to regulate is solved.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of nuclear receptor FXR in targeted therapy of liver cancer stem cells. Background technique [0002] Primary liver cancer is one of the most common malignant tumors clinically, ranking fourth among malignant tumors. The annual death toll due to liver cancer in my country is 42.2 / 10,000, second only to lung cancer and gastric cancer. Hepatocellular carcinoma (Heptocellular carcinoma, HCC) is the most common type of primary liver cancer, accounting for about 85%-90%. The detailed mechanism of liver carcinogenesis is still unclear, but data from leukemia, germ cell tumors and a series of solid tumors support that tumors are maintained by a small population of self-renewing cancer stem cells (CSCs), which is what we usually A well-known tumor stem cell model, CSC plays a decisive role in tumor occurrence, development, invasion, metastasis, recurrence and drug resistance. ...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61P1/16A61P35/00
CPCA61K45/00A61P1/16A61P35/00
Inventor 陈卫东叶文凌王艳东周云聂小博
Owner HENAN UNIVERSITY
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