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Hyaluronic acid purification method

A hyaluronic acid and process technology, which is applied in the field of hyaluronic acid purification technology, can solve problems such as difficulty in dissolving, and achieve the effects of stable product quality, easy production control and high yield.

Inactive Publication Date: 2016-12-21
黄毅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some hyaluronic acid is fibrous in form and difficult to dissolve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The hyaluronic acid purification process includes the following steps:

[0034] (1) Flocculation and filtration: take 25L of fermented liquid and use acetic acid to adjust the pH to 4.7, heat it to 70°C and add flocculant to stir and mix evenly for flocculation. 45min, then carry out vacuum filtration, obtain 24L filtrate;

[0035] (2) Activated carbon decolorization: heat 24L of the filtrate to 72°C, add 144g of activated carbon, stir and decolorize at a speed of 100 rpm for 45min, then vacuum filter to obtain 24L of decolorized solution;

[0036] (3) Ethanol precipitation: the pH of the decolorization solution is adjusted to 6.5 with 2.5mol / L sodium hydroxide solution, and 72L of 95% ethanol is added for precipitation to obtain an ethanol precipitate;

[0037] (4) Complexation precipitation: Dissolve the ethanol precipitate with 25L 0.1mol / L sodium chloride solution, add complexing agent under the condition of stirring at a speed of 300 rpm, stir for 5 minutes after ...

Embodiment 2

[0043] It is basically the same as Example 1, except that the flocculant in the step (1) is obtained by stirring and mixing evenly sodium citrate and sodium gluconate at a mass ratio of 1:1. Obtain the nanoscale hyaluronic acid of embodiment 2.

Embodiment 3

[0045] It is basically the same as Example 1, except that the flocculant in the step (1) is obtained by stirring and mixing evenly sodium alginate and sodium gluconate at a mass ratio of 1:1. Obtain the nanoscale hyaluronic acid of embodiment 3.

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Abstract

The invention discloses a hyaluronic acid purification method. The hyaluronic acid purification method comprises following steps: 1, flocculation filtration is carried out; 2, active carbon decoloration is carried out; 3, ethanol precipitation is carried out; 4, complexation precipitation is carried out; 5, dissociation precipitation is carried out; and 6, an obtained precipitate is dried and is subjected to superfine grinding. Operation process of the hyaluronic acid purification method is simple; a problem of conventional hyaluronic acid purification methods that technology is complex is solved; production is easy to control; product quality is stable; yield is high; cost is reduced greatly; and the application prospect is promising.

Description

technical field [0001] The invention relates to the technical field of biological polysaccharide extraction, in particular to a hyaluronic acid purification process. Background technique [0002] Hyaluronic acid is hyaluronic acid (Sodium Hyaluronate), also known as hyaluronic acid, referred to as HA, which was first isolated from the vitreous humor of the bull's eye by humans in 1934. It is widely present in the extracellular matrix of animal and human connective tissue and some bacteria. A high molecular straight-chain acidic mucopolysaccharide. At present, people have isolated HA from connective tissue, umbilical cord, skin, joint synovial fluid, brain, cartilage, cockscomb, Rana skin and other tissues. In organisms, HA is often combined with proteins and coexists with other mucopolysaccharides. In the vitreous and synovial fluid of the eye, HA exists in a dissolved form, and in the cockscomb and umbilical cord, it exists in the form of a gel. [0003] HA is a chain-li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/0072
Inventor 黄毅
Owner 黄毅