A Novel Comprehensive 2D-LC-MS Method for Metabolite Profiling and Its Detection Kit

Abstract

The present invention discloses a new metabolite profile analysis full-two-dimensional liquid chromatography-mass spectrometry (LC-MS) method, wherein non-transgenic soybean, transgenic soybean, tea leaf and ginkgo leaf are subjected to full-two-dimensional UPLC high-temperature and high-speed analysis by using full-two-dimensional ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (2D-UPLC Q-TOFMS) combined technology to obtain the full-two-dimensional metabolic profile spectrum, and the method has advantages of good reproducibility, high stability, rapidness and reliability, and is suitable for the full-two-dimensional metabolic profile analysis of non-transgenic soybean, transgenic soybean, and a variety of biological samples. The invention further discloses a new kit for detecting the full-two-dimensional metabolic profile spectrum of the metabolite in non-transgenic soybean, transgenic soybean, tea leaf and ginkgo leaf, wherein the kit comprises a buffer liquid ammonium acetate, ammonium formate or ammonium bicarbonate, 0.1-0.5% formic acid or acetic acid water, and acetonitrile. According to the present invention, the method for detecting the full-two-dimensional metabolic profile spectrum of non-transgenic soybean, transgenic soybean, and a variety of biological samples by using the kit has characteristics of low detection cost, good reproducibility, high stability, rapidness, and high efficiency.

Description

technical field The invention relates to the field of analytical chemistry, and is obtained by using a comprehensive two-dimensional ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry technology to conduct comprehensive two-dimensional UPLC high-temperature and high-speed analysis on non-transgenic soybeans, genetically modified soybeans, tea leaves and ginkgo leaves. Its new method for comprehensive two-dimensional metabolic profiling. At the same time, the detection kit disclosed in the present invention is a new technology for comprehensive two-dimensional metabolic profiling analysis of metabolites in various biological samples such as non-transgenic soybeans and transgenic soybeans. Background technique In recent years, modern chromatography has become one of the important tools for the analysis and separation of complex system components in the field of analytical chemistry. For the separation of components in complex samples, on...

AI Technical Summary

Problems solved by technology

Compared with the off-line two-dimensional liquid chromatography technology with complex operation, tedious procedures, and easy loss and contamination of sample components, comprehensive two-dimensional online liquid chromatography technology has attracted more and more attention.

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