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New metabolite profile analysis full-two-dimensional liquid chromatography-mass spectrometry (LC-MS) method and detection kit thereof

A metabolite profile and metabolite profile technology, which is applied in the analysis of materials, measurement devices, material separation, etc., can solve the problems of complicated procedures, complicated operations, and easy loss, and achieve simple preprocessing, rapid analysis, and avoidance of human errors. Effect

Inactive Publication Date: 2016-12-21
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Compared with the off-line two-dimensional liquid chromatography technology with complex operation, tedious procedures, and easy loss and contamination of sample components, comprehensive two-dimensional online liquid chromatography technology has attracted more and more attention.

Method used

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  • New metabolite profile analysis full-two-dimensional liquid chromatography-mass spectrometry (LC-MS) method and detection kit thereof
  • New metabolite profile analysis full-two-dimensional liquid chromatography-mass spectrometry (LC-MS) method and detection kit thereof
  • New metabolite profile analysis full-two-dimensional liquid chromatography-mass spectrometry (LC-MS) method and detection kit thereof

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Experimental program
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Embodiment 1

[0030] 1. Collection of non-genetically modified soybeans, genetically modified soybeans, tea leaves and ginkgo biloba samples

[0031] Purchase non-genetically modified soybeans, genetically modified soybeans, some scented tea leaves, and collect some fresh ginkgo leaves, and immediately store them in an ultra-low temperature -80°C refrigerator for later use.

[0032] 2. Analysis method

[0033] 2.1 Sample preprocessing

[0034] Non-genetically modified soybeans, genetically modified soybeans, tea leaves, and ginkgo biloba samples were taken out of the ultra-low temperature refrigerator and thawed at room temperature. Stir and grind into powder with liquid nitrogen, freeze-dry and weigh, accurately weigh 60mg of sample into a 2mL centrifuge tube, add 1.5mL of 70% methanol water for ultrasonication for 1 hour, centrifuge at 12000 rpm for 15 minutes, filter with a 0.22μm organic filter membrane and directly Loading analysis.

[0035] 2.2 Comprehensive two-dimensional ultra-h...

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Abstract

The present invention discloses a new metabolite profile analysis full-two-dimensional liquid chromatography-mass spectrometry (LC-MS) method, wherein non-transgenic soybean, transgenic soybean, tea leaf and ginkgo leaf are subjected to full-two-dimensional UPLC high-temperature and high-speed analysis by using full-two-dimensional ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (2D-UPLC Q-TOFMS) combined technology to obtain the full-two-dimensional metabolic profile spectrum, and the method has advantages of good reproducibility, high stability, rapidness and reliability, and is suitable for the full-two-dimensional metabolic profile analysis of non-transgenic soybean, transgenic soybean, and a variety of biological samples. The invention further discloses a new kit for detecting the full-two-dimensional metabolic profile spectrum of the metabolite in non-transgenic soybean, transgenic soybean, tea leaf and ginkgo leaf, wherein the kit comprises a buffer liquid ammonium acetate, ammonium formate or ammonium bicarbonate, 0.1-0.5% formic acid or acetic acid water, and acetonitrile. According to the present invention, the method for detecting the full-two-dimensional metabolic profile spectrum of non-transgenic soybean, transgenic soybean, and a variety of biological samples by using the kit has characteristics of low detection cost, good reproducibility, high stability, rapidness, and high efficiency.

Description

technical field [0001] The invention relates to the field of analytical chemistry, and is obtained by using a comprehensive two-dimensional ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry technology to conduct comprehensive two-dimensional UPLC high-temperature and high-speed analysis on non-transgenic soybeans, genetically modified soybeans, tea leaves and ginkgo leaves. Its new method for comprehensive two-dimensional metabolic profiling. [0002] At the same time, the detection kit disclosed in the present invention is a new technology for comprehensive two-dimensional metabolic profiling analysis of metabolites in various biological samples such as non-transgenic soybeans and genetically modified soybeans. Background technique [0003] In recent years, modern chromatography has become one of the important tools for the analysis and separation of complex system components in the field of analytical chemistry. For the separation of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 王文昭刘杏忠李二伟任晋玮
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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