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A detection method for the quality identification of pangolin scales

A quantitative detection method and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor specificity, difficulty in detecting pangolin scales by ion chromatography, and low sensitivity, so as to ensure drug safety and consumption the effect of interest

Active Publication Date: 2018-01-30
广东省药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the pangolin quality standards and related research materials, the purity of the samples is controlled by checking the total ash content, but this method is not specific and sensitive; on the other hand, it is difficult to detect the Pangolin scales eluted after weight gain

Method used

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  • A detection method for the quality identification of pangolin scales
  • A detection method for the quality identification of pangolin scales
  • A detection method for the quality identification of pangolin scales

Examples

Experimental program
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preparation example Construction

[0034] 1.1 Preparation of ion content standard curve: Accurately measure the single element standard solution (1000 μg / mL), add water to make a series of mixed standard solutions containing 0.1 μg, 1 μg, 5 μg, 10 μg, 50 μg, and 100 μg of salt ions in each 1 mL, and carry out For chromatograms, prepare a standard curve for ion chromatograms based on the relative peak areas.

[0035] 1.2 Chromatographic conditions:

[0036] a. Anion chromatography: The chromatographic column is an anion exchange column IonPac AS11-HC (4×250mm), the eluent concentration is 0-30min 10mmol / L potassium hydroxide solution, a conductivity detector, and the flow rate is 1.0mL / min. The detection method is anion-suppressed conductivity detection, and the injection volume is 25 μL.

[0037] b. Cation chromatography: The chromatographic column is a cation exchange column IonPac CS12A (4×250mm), the eluent concentration is 0-20min 5mmol / L sulfuric acid solution, the conductivity detector, the flow rate is ...

Embodiment 1

[0039] The quality detection (1) of embodiment 1 pangolin scales

[0040] The pangolin standard and NaCl, MgSO 4 1. The pangolin to be tested after KCl weight-increasing treatment was crushed into powder, weighed 1.5g of pangolin powder, first dissolved with 50mL of ethyl acetate at 60°C, fully mixed and stirred, then dried, redissolved with 25mL of water, fully Shake for 30 minutes, and freeze at -20°C for 4 hours. Add water to make up to 100mL, shake well and filter for ion chromatography. The chromatographic conditions were as described in the comparative example.

Embodiment 2

[0041] The quality detection (2) of embodiment 2 pangolin scales

[0042] The pangolin standard and NaCl, MgSO 4 1. After the KCl weight-increasing treatment, the pangolin to be tested was crushed into powder, and 1.5g of the pangolin powder was weighed, redissolved in 25mL of water, fully shaken for 30min, and frozen at -15°C for 4h. Add water to make up to 100mL, shake well and filter for ion chromatography. The chromatographic conditions were as described in the comparative example.

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Abstract

The invention discloses a detection method capable of rapidly identifying the pangolin scale quality. The detection method comprises: 1) grinding a product to be detected, and dissolving with an organic solvent; 2) drying the organic solvent for dissolving, re-dissolving with water, and carrying out a low temperature treatment; and 3) analyzing the sample by using an ion chromatography method. The method of the present invention has advantages of simpleness, convenience, high precision and good effect, and can overcome the defects of the routine detection methods.

Description

technical field [0001] The invention relates to a detection method for quality identification of traditional Chinese medicines, in particular to a detection method and application for quality identification of pangolin scales, and belongs to the fields of drug analysis technology and drug quality control. Background technique [0002] Pangolin is derived from the scales of Manis pentadactyla Linnaeus, an animal of the dace carp family. It has the functions of promoting blood circulation and eliminating symptoms, dredging menstrual flow and breast milk, reducing swelling and draining pus, searching wind and dredging collaterals, and is used for amenorrhea, blocked milk, carbuncle and sore Poison, rheumatic arthralgia, stroke paralysis, numbness and spasm embolism. Its processed products are generally used clinically: Pao Shan Jia and Vinegar Shan Jia. Paogolin is made by scalding raw pangolin; vinegar golin is made by scalding and quenching with vinegar. [0003] The pangol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/96
CPCG01N30/96
Inventor 刘潇潇罗卓雅李华黄国凯张丽丹
Owner 广东省药品检验所