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Tissue-culture industrialized seedling production method of dendrobium officinale

A technology of Dendrobium officinale and industrialization, applied in the directions of gardening methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of yellowing, death, browning of seedlings, etc. The effect of factory production efficiency

Inactive Publication Date: 2017-01-04
耿马四方生物科技开发有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing dendrobium tissue culture industrial seedlings basically refer to the medium, additives and culture conditions produced by other Orchidaceae. Although the production has a certain efficiency, there are still protocorms, cluster buds, and browning and yellowing of seedlings in the seedling cultivation process. waiting for death

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The method of tissue culture factory seedlings of Dendrobium officinale includes the following steps:

[0022] (1) Provenance selection: From June to September during the peak season of Dendrobium growth, single-cluster seedlings with few diseases and insect pests and robust growth are marked one by one. EM bacteria, calcium fulvic acid and brassin are sprayed three times a month before collection. During the harvest season from November to March, the marked and capped mature seedlings shall be inspected for the corresponding physical and chemical indicators of the Chinese Pharmacopoeia, and the stems shall be collected for the seedlings that meet the standards of the Pharmacopoeia;

[0023] (2) Explant pretreatment: Put the fresh stems of Dendrobium into 35℃ warm water with a proper amount of detergent and soak for 60 minutes. Peel off the leaf sheaths of the soaked stems of Dendrobium so as not to damage the latent buds on the nodes. Cut the stem segment from the peeled le...

Embodiment 2

[0031] The method of tissue culture factory seedlings of Dendrobium officinale includes the following steps:

[0032] (1) Provenance selection: From June to September during the peak season of dendrobium growth, single-cluster seedlings with few diseases and insect pests and robust growth are marked one by one. EM bacteria, calcium fulvic acid and brassin are sprayed three times a month before collection. During the harvest season from November to March, the marked and capped mature seedlings shall be inspected for the corresponding physical and chemical indicators of the Chinese Pharmacopoeia, and the stems shall be collected for the seedlings that meet the standards of the Pharmacopoeia;

[0033] (2) Explant pretreatment: Put the fresh stems of Dendrobium stalks into 35~45℃ warm water with an appropriate amount of detergent and soak for 35 minutes, peel off the leaf sheaths of the soaked stems of Dendrobium so that it can not damage the nodes. The buds and stems from the leaf she...

Embodiment 3

[0041] The method of tissue culture factory seedlings of Dendrobium officinale includes the following steps:

[0042] (1) Provenance selection: From June to September during the peak season of dendrobium growth, single-cluster seedlings with few diseases and insect pests and robust growth are marked one by one. EM bacteria, calcium fulvic acid and brassin are sprayed three times a month before collection. During the harvest season from November to March, the marked and capped mature seedlings shall be inspected for the corresponding physical and chemical indicators of the Chinese Pharmacopoeia, and the stems shall be collected for the seedlings that meet the standards of the Pharmacopoeia;

[0043] (2) Explant pretreatment: Put fresh stems of Dendrobium stalks into 35~45℃ warm water with a proper amount of detergent and soak for 55 minutes, peel off the leaf sheaths of the soaked stems of Dendrobium so as not to damage the nodes. The buds and stems from the leaf sheaths were cut in...

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Abstract

The invention discloses a tissue-culture industrialized seedling production method of dendrobium officinale, and relates to the technical field of biology. According to the tissue-culture industrialized seedling production method of the dendrobium officinale, field cluttered germplasms are screened through a simple and easy method, and dendrobium officinale stem segments which are high in yield, disease-resistant and good in appearance and taste and of which the quality meets the standards of Chinese Pharmacopoeia are screened out to greatly shorten the variety screening time; in the culture process, a large number of elements in an MS culture medium are improved, fulvic acid calcium and a new food antioxidant of sodium erythorbate or calcium ascorbate are added in additives, the culture condition is that lighting of an led light source of which the color temperature is 4000K is used, and small seedlings of certain specifications are circularly transferred in the strong seedling production process, so that the growth rate of tissue culture seedlings is improved, and the states of small seedling browning, yellowing and undergrowth and the turnover frequency of the tissue culture seedlings are reduced in the production process to improve the industrialized production efficiency.

Description

Technical field [0001] The present invention relates to the field of biological technology, and more specifically, the present invention relates to a method of tissue culture factory seedlings of Dendrobium candidum. Background technique [0002] Dendrobium officinale is widely used clinically and in traditional Chinese medicine compounds. The application of Dendrobium officinale is mainly based on the collection of wild resources. With the depletion of wild resources, artificial Dendrobium officinale has been planted in large numbers. At present, most artificial planting seedlings of Dendrobium candidum come from ramets, cuttings and aseptic sowing and propagation of seeds. The first two methods consume a lot of resources and develop slowly. The latter method belongs to sexual propagation and has a certain production efficiency. However, this method has large seedling variability and inconsistent properties, resulting in too much fluctuation in the physical and chemical indicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘中侨
Owner 耿马四方生物科技开发有限责任公司
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