Unlock instant, AI-driven research and patent intelligence for your innovation.

A method of using lactic acid bacteria to degrade mycotoxins in food

A technology of mycotoxins and lactic acid bacteria, applied in the direction of food science, can solve the problems that no one has made, and achieve the effect of reducing pollution

Active Publication Date: 2019-10-18
GUANGDONG OCEAN UNIVERSITY
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In recent years, researches at home and abroad can see the use of specific strains combined with mycotoxins to degrade AFB in liquid matrices. 1 Compared with T-2 toxin, whether lactic acid bacteria in yogurt can degrade AFB1 and T-2 toxin has not yet been researched

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method of using lactic acid bacteria to degrade mycotoxins in food
  • A method of using lactic acid bacteria to degrade mycotoxins in food
  • A method of using lactic acid bacteria to degrade mycotoxins in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Isolation and identification of Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacillus casei

[0026] Dilute the fermented milk as concentration gradient, choose 10 -3 、10 -4 、10 -5 Spread 0.1 mL of each of the three dilutions on the prepared MRS medium, incubate at 37°C for 48 hours, observe the colony shape after 48 hours, and inoculate the suspected colonies of Lactobacillus bulgaricus and Lactobacillus acidophilus in the On the MRS medium, culture at 37°C for 48 hours, observe the colony morphology after 48 hours, and take pictures.

[0027] Dilute the lactic acid bacteria milk drink as a concentration gradient, and select 10 -3 、10 -4 、10 -5 Spread 0.1 mL of each of the three dilutions on the prepared MRS medium, incubate at 37°C for 48 hours, observe the colony morphology after 48 hours, and take pictures. Streak inoculation of Lactobacillus casei colonies in MRS medium, culture at 37°C for 48 hours, observe the colony morphology and ta...

Embodiment 2

[0036] Example 2 The combined effect of lactic acid bacteria on AFB 1 Degradation experiment of mixed toxin with T-2 toxin

[0037] 1.0mg AFB 1 The toxin standard was dissolved in acetonitrile, and placed in a 10mL volumetric flask to volume with acetonitrile. Prepare a standard stock solution with a concentration of 0.1 mg / mL. Take 1mL of the stock solution and dilute to the volume with methanol in a 10mL volumetric flask to obtain AFB with a concentration of 10μg / mL 1 Toxin standard solution. Prepared standard solutions were stored in a -20°C refrigerator.

[0038] AFB as needed 1 Prepare standard solutions with concentrations of 250, 500, 1000, 2500, and 5000 ng / mL in the standard solutions; the standard solutions are ready-to-use and the solvent is 30% methanol solution, and the prepared standard solutions are stored in a -20°C refrigerator.

[0039] According to the minimum detection standard of T-2 toxin, 5 concentration gradients were also set. Dissolve 1.0mg T-2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides the application of lactic acid bacteria in degrading mycotoxins in food, said lactic acid bacteria are Lactobacillus bulgaricus, Lactobacillus acidophilus and Lactobacillus casei, and said mycotoxin is AFB 1 and / or T‑2 toxin, by co-cultivation to study the effect of Lactobacillus bulgaricus, Lactobacillus acidophilus and Lactobacillus casei on AFB 1 And the similarities and differences in the degradation of T-2 toxin; this research can be effectively applied to actual life production, and will effectively reduce AFB 1 And the contamination of food by T-2 toxin, it has theoretical guiding significance and practical value for the degradation of aflatoxin and T-2 toxin in the modern production of food.

Description

technical field [0001] The invention relates to the technical field of toxin degradation, and more particularly relates to a method for degrading mycotoxins in food by using lactic acid bacteria. Background technique [0002] Aflatoxins are a general term for a class of compounds with similar chemical structures, which are derivatives of dihydrofuranocoumarins. Aflatoxins are mainly produced by Aspergillus flavus ( A. flavus ) and Aspergillus parasitica ( A. parasiticus ) produced secondary metabolites, is the world's most recognized natural carcinogenic toxins, in 1993 by the World Health Organization (WHO) Cancer Research Institute designated as I carcinogens. The mycotoxin pollution rate in my country is as high as 90%, and the mold pollution is particularly serious in the south of China where the climate is warm and humid. Aflatoxins are widely found in food, agricultural products and animal feed, and seriously pollute grain and oil products such as wheat and corn. A...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A23L5/20
CPCA23V2400/125A23V2400/123A23V2400/113
Inventor 王雅玲孙力军周浪花刘颖
Owner GUANGDONG OCEAN UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com