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A method for comprehensively extracting cordycepin and cordyceps polysaccharide from Cordyceps militaris by two aqueous phases

A comprehensive extraction and two-phase technology, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of scarcity of wild resources and achieve broad product prospects, fast phase separation, high selectivity and specificity single effect

Active Publication Date: 2019-02-26
SHANDONG JIANZHU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Cordyceps is a traditional Chinese medicine in my country, especially Cordyceps sinensis is the most famous, but so far Cordyceps sinensis has not been artificially cultivated on a large scale, and the increasing demand and high prices in the market have led to excessive collection of wild Cordyceps sinensis, making wild resources increasingly scarcity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Dry the fruiting bodies of Cordyceps militaris at 65°C to constant weight, crush them, and pass through a 100-mesh sieve to obtain Cordyceps militaris powder.

[0022] 2. PEG / (NH 4 ) 2 SO 4 The aqueous two-phase consists of 35% polyethylene glycol 6000, 25% (NH 4 ) 2 SO 4 , 18% NaCl and the balance of deionized water.

[0023] 3. Add 10g of Cordyceps militaris powder to 400ml of PEG / (NH 4 ) 2 SO 4 In the two-phase aqueous system, microwave-assisted extraction is adopted, the extraction temperature of microwave-assisted extraction is 40°C, the extraction time is 20 minutes, and the microwave power is 200W. Stand at room temperature for 10 minutes to separate the phases to obtain upper and lower phases.

[0024] 4. The upper liquid phase in the obtained two-water phase was centrifuged for 2 minutes at a speed of 5000 rpm, and its precipitate was removed to obtain a supernatant liquid, which was washed with ethyl acetate and sherwood oil successively, and vacuu...

Embodiment 2

[0027] 1. Dry the fruiting bodies of Cordyceps militaris at 65°C to constant weight, crush them, and pass through a 100-mesh sieve to obtain Cordyceps militaris powder.

[0028] 2. PEG / (NH 4 ) 2 SO 4 The aqueous two-phase consists of 35% polyethylene glycol 6000, 25% (NH 4 ) 2 SO 4 , 18% NaCl and the balance of deionized water.

[0029] 3. Add 10g of Cordyceps militaris powder to 400ml of PEG / (NH 4 ) 2 SO 4 In the two-phase aqueous system, microwave-assisted extraction is adopted, the extraction temperature of microwave-assisted extraction is 40°C, the extraction time is 40 minutes, and the microwave power is 200W. Stand at room temperature for 20 minutes to separate the phases to obtain upper and lower phases.

[0030] 4. The upper liquid phase in the obtained two-water phase was centrifuged for 2 minutes at a speed of 5000 rpm, and its precipitate was removed to obtain a supernatant liquid, which was washed with ethyl acetate and sherwood oil successively, and vacuu...

Embodiment 3

[0033] The method of Example 1 was used to comprehensively extract cordycepin and cordyceps polysaccharides in Cordyceps militaris by using two aqueous phases, the difference was that in step 3, the microwave-assisted extraction time was 30 minutes, and the phase separation was allowed to stand at room temperature for 20 minutes. Finally, the extraction rate of cordycepin was 51.5%, and the extraction rate of cordyceps polysaccharide was 33.8%.

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Abstract

The invention discloses a double-aqueous-phase comprehensive extraction method for cordycepin and cordyceps polysaccharide in cordyceps militaris. The method includes the steps of (1) raw material pretreating; (2) double-aqueous-phase system preparing; (3) double-aqueous-phase extracting; (4) cordycepin extracting; (5) cordyceps polysaccharide extracting. The double-aqueous-phase comprehensive extraction method for the cordycepin and the cordyceps polysaccharide in the cordyceps militaris is simple in technology process, convenient to operate and low in cost; the cordycepin and the cordyceps polysaccharide in the cordyceps militaris can be extracted at the same time, and resource wasting and cordyceps-militaris deep processing cost reducing which are caused by single extracting are effectively avoided.

Description

technical field [0001] The invention relates to a method for comprehensively extracting cordycepin and cordyceps polysaccharides from Cordyceps militaris with two aqueous phases, and belongs to the field of separation and purification of effective components of natural products. Background technique [0002] Cordyceps is a traditional Chinese medicine in my country, especially Cordyceps sinensis is the most famous, but so far Cordyceps sinensis has not been artificially cultivated on a large scale, and the increasing demand and high prices in the market have led to excessive collection of wild Cordyceps sinensis, making wild resources increasingly scarcity. Modern scientific research has proved that Cordyceps militaris has similar effects to Cordyceps sinensis, and some active ingredients are even higher than Cordyceps sinensis. Because Cordyceps militaris is easy to cultivate, it is generally used to produce Cordyceps fruiting body and mycelium to develop a series of Cordyc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C07H19/16C07H1/08
Inventor 张超陈文兵武道吉侯书国闫浩朱海龙袁永佳
Owner SHANDONG JIANZHU UNIV