A method for purifying bovine fetal skeletal muscle tissue-derived preadipocytes
A fat cell, skeletal muscle technology, applied in the field of biochemistry, can solve problems such as inability to obtain and reduce body fat
Active Publication Date: 2019-11-08
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology
Given the many articles published each year on lipid metabolism and adipogenesis, it is largely impossible to obtain an effective method of reducing body fat or reducing adipocyte differentiation through exogenous treatments
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Embodiment 1
[0052] A method for purifying bovine fetal skeletal muscle tissue-derived preadipocytes, the material used in the method is 3-month-old fetal longissimus dorsi muscle tissue, and the reagents used are collagenase, fetal bovine serum, low-sugar Dulbecco 's modified Eagle's medium, cell sorting buffer, anti-platelet-derived growth factor receptor alpha antibody, dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, and Oil Red O.
[0053] The method includes the following steps:
[0054] Step 1. Cell isolation and culture;
[0055] Step 2, cell culture;
[0056] Step 3. Adipogenic induction of cells;
[0057] Step 4, oil red O;
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The invention discloses a method for purifying preadipocytes derived from fetal bovine skeletal muscle tissue. According to the method, a used material is longissimus dorsi tissue of a 3-month-old bovine fetus, and used reagents contain collagenase, fetal bovine serum, the low- glucose Dulbecco's Modified Eagle's Medium, a cell sorting buffer solution, a PDGFR-alpha (platelet-derived growth factor receptor alpha) antibody, dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin and oil red O.
Description
technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a method for purifying bovine fetal skeletal muscle tissue-derived preadipocytes. Background technique [0002] Stem cells are a class of cells that can maintain the ability to replicate and differentiate into different types of cells. Stem cell research is very important for understanding the regulation of cell and tissue development and differentiation. In addition, stem cell research also has great potential for the medical field. Like many other organs, skeletal muscle contains many cell types that give rise to muscle-derived satellite cells and adipose tissue-derived adipocytes, both of which are important for animal production. It is well known that satellite cells are important for both postnatal skeletal muscle growth and skeletal muscle regeneration in adults. Research on isolated satellite cells over the past nearly 50 years has focused on activation ...
Claims
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IPC IPC(8): C12N5/077
CPCC12N5/0653C12N2500/40C12N2501/135C12N2501/33C12N2509/00
Inventor 张路培胡鑫高会江李俊雅
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI