Method for extracting small molecular peptide from periplaneta americana

Abstract

The invention relates to a method for extracting small molecular peptides from periplaneta americana. The method for extracting small molecular peptides from the periplaneta americana is characterized in that the method comprises the following steps of (1) crushing raw materials in advance into 30mesh to 40mesh powder; (2) extracting serous liquid by adopting dynamic micro-pressure; (3) performing complex enzyme gradient enzymolysis: increasing temperature for sterilization, and then decreasing temperature to 50DEG C, adding 100U / g xylanase for enzymolysis for 1 to 2 hours; then adding 150U / g pectinase for enzymolysis for 1 to 2 hours; then adding NaOH solution to regulate pH to 6.0, and adding 3200U / g papain for enzymolysis for 2 to 3 hours; (4) performing membrane separation, filtering to clear liquid and storing the clear liquid in a tank; (5) performing double-effect vacuum energy-saving concentration, wherein first-effect vacuum degree and temperature are respectively controlled to be negative 0.06MPa and 70 to 80DEG C, and second-effect vacuum degree and temperature are respectively controlled to be negative 0.08MPa and 50 to 60DEG C; (6) performing high-speed centrifugal spray drying; (7) packaging finished products. By implementing the technical scheme, the method for extracting small molecular peptides from the periplaneta americana has the technical effects that the cost is low, the effective component extraction rate is high, the molecular weight of the extract is small and the absorption and utilization are very easy to realize.

Description

technical field [0001] The invention belongs to a method for extracting peptides or proteins, and in particular relates to a method for extracting small molecule peptides of the American cockroach by using a biological enzymatic hydrolysis technology using dried Periplaneta americana as raw materials. Background technique [0002] Periplaneta americana, commonly known as cockroach, is one of the most vigorous, oldest and most successful insect groups in the world. Periplaneta americana is cold in nature, salty in taste, poisonous, pungent, and has the functions of dispelling blood stasis, eliminating stagnation, detoxifying, diuresis, and reducing swelling. Modern research shows that it also has anticancer effects. [0003] Periplaneta americana (cockroach) is a traditional Chinese medicinal material. It is often used as medicine with dried or fresh adults. There are also Periplaneta americana fine powder made into ultrafine powder, which is used in health care products or ...

AI Technical Summary

Problems solved by technology

However, due to traditional habits, it is difficult for patients to accept it, and it is difficult for the drug effect to be fully exerted.

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