Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting and separating ursolic acid from olive leaves

A technology of olive leaf and ursolic acid, applied in the direction of steroids, organic chemistry, etc., can solve the problems of difficult industrial production process, poor continuous operability, long processing cycle, etc., and achieve short cycle, simple method and high yield big effect

Active Publication Date: 2018-03-16
桂林益天成生物科技有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The triterpenoids in olive leaves mainly include oleanolic acid, maslinic acid and ursolic acid, and these three substances have similar structures, and are usually separated by column chromatography, but repeated silica gel column chromatography separation and recrystallization lead to the final The amount of sample obtained is small
And because column chromatography generally has a long processing period and poor continuous operability, it is not easy to be used as a separation medium for the industrial production process of biologically active substances.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Crush olive leaves to 100 mesh, mix with ethanol solution with a volume concentration of 70% according to the ratio of 1g: 10ml, heat and reflux for extraction for 3 hours, filter the extract, take the filtrate to recover ethanol under reduced pressure, and obtain crude Extract;

[0017] 2) Add the crude extract to a 50% ethanol solution until the concentration of the crude extract is 0.06 g / ml, and stir well to obtain a mixed solution;

[0018] 3) Put the mixed solution on the AB-8 macroporous adsorption resin column, the ratio of diameter to height of the resin column is 1:3, first elute with water until colorless, then add 40°C, 0.1mol / L sodium bicarbonate solution for elution , the elution rate of sodium bicarbonate solution is 1BV / h, and its dosage is 1BV, and then eluted with 75°C, 0.6mol / L sodium bicarbonate solution, the elution rate is 2BV / h, and its dosage is 0.5BV , collect the third eluate (that is, 0.6 mol / L sodium bicarbonate eluate), adjust the pH to ...

Embodiment 2

[0021] 1) Crush olive leaves to 180 mesh, mix with ethanol solution with a volume concentration of 90% according to the ratio of 1g to 20ml, heat and reflux for extraction 3 times, each extraction time is 3h, filter the extract, and take The filtrate reclaims ethanol under reduced pressure to obtain a crude extract;

[0022] 2) Add the crude extract to an ethanol solution with a volume concentration of 65% until the concentration of the crude extract is 0.10 g / ml, and stir well to obtain a mixed solution;

[0023] 3) Put the mixed solution on the AB-8 macroporous adsorption resin column, the ratio of diameter to height of the resin column is 1:6, first elute with water until colorless, then add 50°C, 0.15mol / L sodium bicarbonate solution for elution , the elution rate of sodium bicarbonate solution is 1.5BV / h, the dosage is 1.5BV, and then eluted with 78°C, 0.8mol / L sodium bicarbonate solution, the elution speed is 2.5BV / h, and the dosage For 1BV, collect the third eluate (th...

Embodiment 3

[0026] 1) Crush olive leaves to 150 mesh, mix with ethanol solution with a volume concentration of 90% according to the ratio of 1g to 20ml, heat and reflux for extraction twice, each extraction time is 2h, filter the extract, and take The filtrate reclaims ethanol under reduced pressure to obtain a crude extract;

[0027] 2) adding the crude extract to an ethanol solution with a volume concentration of 55% until the concentration of the crude extract is 0.08 g / ml, and stirring well to obtain a mixed solution;

[0028] 3) Put the mixed solution on the AB-8 macroporous adsorption resin column, the ratio of diameter to height of the resin column is 1:4, first elute with water until colorless, then add 45°C, 0.12mol / L sodium bicarbonate solution for elution , the elution rate of sodium bicarbonate solution is 1.2BV / h, the dosage is 1.2BV, and then eluted with 76°C, 0.7mol / L sodium bicarbonate solution, the elution rate is 2.5BV / h, and the dosage is 0.5BV, collect the third eluat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
specific surface areaaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for extracting ursolic acid from olive leaves. The method comprises the following steps: 1) pulverizing olive leaves, extracting with a 70-90 vol% ethanol solution, filtering the extracting solution, taking the filtrate, and recovering the ethanol under reduced pressure to obtain a crude extract; 2) adding the crude extract into a 50-65 vol% ethanol solution to be redissolved, thereby obtaining a mixed solution; and 3) passing the mixed solution through an AB-8 macroporous adsorbent resin column, eluting with water to a colorless state, eluting with a 40-50-DEG C 0.1-0.15 mol / L sodium bicarbonate solution, eluting with a 75-78-DEG C 0.6-0.8 mol / L sodium bicarbonate solution, collecting the three eluates, regulating the pH value to 3-5, filtering, taking the filter residue, and drying. The ursolic acid extracted by the method has the advantages of high purity and high yield.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for extracting and separating ursolic acid from olive leaves. Background technique [0002] Olive (Olea europare L.) is a world-famous woody oil tree species with a cultivation history of more than 4,000 years. The decoction or infusion of olive leaves is often used as folk medicine for diabetes in Mediterranean countries and has certain curative effects. Olive leaf extract is used as a dietary supplement in the Americas and Europe to enhance immune function. Modern data show that olive leaves contain active ingredients such as flavonoids, triterpenes, sugars, and iridoids. Triterpenoids are mainly composed of 6 isoprene structural units, most of which are terpenoids with 30 carbon atoms. The triterpenoids in olive leaves mainly include oleanolic acid, maslinic acid and ursolic acid, and these three substances have similar structures, and are...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 黄健军谢冬养陆美珍叶出良
Owner 桂林益天成生物科技有限公司