Method for determining germination rate of spores of sphaeropsis sapinea of pinus tabuliformis

A technology of spore germination rate and germination rate, applied in the field of fungi

Inactive Publication Date: 2017-01-25
HEBEI AGRICULTURAL UNIV.
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Problems solved by technology

The pathogen is not easy to produce pycnidia on artificial culture medium, but it is easy t

Method used

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  • Method for determining germination rate of spores of sphaeropsis sapinea of pinus tabuliformis
  • Method for determining germination rate of spores of sphaeropsis sapinea of pinus tabuliformis
  • Method for determining germination rate of spores of sphaeropsis sapinea of pinus tabuliformis

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Embodiment Construction

[0026] 1 Materials and methods

[0027] 1.1 Tested strains: Pinus tabulaeformis came from the Forest Pathology Laboratory of Hebei Agricultural University and stored in a -20°C refrigerator for later use.

[0028] 1.2 Induction of pycnidia of Pinus tabulaeformis

[0029] After the strains to be tested were grown on the potato dextrose agar medium for 3 days, the inoculation was subjected to three consecutive high-pressure sterilizations (the pine needles were rinsed with tap water, air-dried, cut into small sections of about 1 cm, placed in a petri dish, added a small amount of distilled water to Keep it moist, 121 ℃ autoclave 25min) of pine needles, cultivate in 25 ℃ constant temperature light incubator, after the pycnidia and conidia mature, take out the pine needles, place them in a sterile petri dish for later use.

[0030] 1.3 Determination of conidia germination rate of Pinus tabulaeformis by hanging drop method

[0031] 1.3.1 Preparation of spore suspension. The conid...

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Abstract

The invention provides a method for determining the germination rate of spores of sphaeropsis sapinea of pinus tabuliformis. The method comprises the following steps of sterilizing the surfaces of plant tissues of the pinus tabuliformis with a sphaeropsis sapinea pycnidium, culturing, observing a germination situation of the pycnidium through using an optical microscope during a culturing process, and calculating a germination rate of the pycnidium; acquiring the germination rate of the spores through calculating according to the following formula: the germination rate of the spores is equal to 0.195 + 0.864* the germination rate of the pycnidium. According to the method provided by the invention, a linear relation exists between the germination rate of the pycnidium of the sphaeropsis sapinea of the pinus tabuliformis and the germination rate of the spores, and can be expressed through a one-unknown-quantity regression equation approximatively, the germination rate of the spores of the sphaeropsis sapinea of the pinus tabuliformis is measured by utilizing the regression equation, and a test is carried out on a predicted value and an actual value of the germination rate of the spores so as to discover that no significant difference exists between the predicted value and the actual value, so that the method of measuring and calculating the germination rate of the spores by utilizing the germination rate of the pycnidium has a scientific basis, is simple and convenient, and can be used as a substituting method for determining the germination rate of the spores.

Description

technical field [0001] The invention belongs to the technical field of fungi, and in particular relates to a method for measuring the germination rate of spores of pine dieback fungus. Background technique [0002] Spore germination is an important process in the study of fungal diseases in plant pathology. For example, spore germination is an important part of the study of fungal biological characteristics. At the same time, the spore germination inhibition test is also a necessary indicator for the screening of biocontrol bacteria and chemical agents. In addition, the germination of spores is involved in the study of fungal life history, disease infection process, infection cycle and epidemic prediction. [0003] The traditional methods for measuring spore germination mainly include hanging drop method, water agar surface germination method, petri dish germination method, agar plate surface germination method, etc., but these methods all need to obtain a large amount of pu...

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Application Information

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IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 李会平任晓婧崔建州苏筱雨李英董亚新王雪菲暴可心张思涵
Owner HEBEI AGRICULTURAL UNIV.
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