Method for determining germination rate of spores of sphaeropsis sapinea of pinus tabuliformis

A technology of spore germination rate and germination rate, applied in the field of fungi
CN106350573AInactive Publication Date: 2017-01-25HEBEI AGRICULTURAL UNIV.
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HEBEI AGRICULTURAL UNIV.
Publication Date
2017-01-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for determining the germination rate of spores of sphaeropsis sapinea of pinus tabuliformis. The method comprises the following steps of sterilizing the surfaces of plant tissues of the pinus tabuliformis with a sphaeropsis sapinea pycnidium, culturing, observing a germination situation of the pycnidium through using an optical microscope during a culturing process, and calculating a germination rate of the pycnidium; acquiring the germination rate of the spores through calculating according to the following formula: the germination rate of the spores is equal to 0.195 + 0.864* the germination rate of the pycnidium. According to the method provided by the invention, a linear relation exists between the germination rate of the pycnidium of the sphaeropsis sapinea of the pinus tabuliformis and the germination rate of the spores, and can be expressed through a one-unknown-quantity regression equation approximatively, the germination rate of the spores of the sphaeropsis sapinea of the pinus tabuliformis is measured by utilizing the regression equation, and a test is carried out on a predicted value and an actual value of the germination rate of the spores so as to discover that no significant difference exists between the predicted value and the actual value, so that the method of measuring and calculating the germination rate of the spores by utilizing the germination rate of the pycnidium has a scientific basis, is simple and convenient, and can be used as a substituting method for determining the germination rate of the spores.
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Description

technical field

[0001] The invention belongs to the technical field of fungi, and in particular relates to a method for measuring the germination rate of spores of pine dieback fungus. Background technique

[0002] Spore germination is an important process in the study of fungal diseases in plant pathology. For example, spore germination is an important part of the study of fungal biological characteristics. At the same time, the spore germination inhibition test is also a necessary indicator for the screening of biocontrol bacteria and chemical agents. In addition, the germination of spores is involved in the study of fungal life history, disease infection process, infection cycle and epidemic prediction.

[0003] The traditional methods for measuring spore germination mainly include hanging drop method, water agar surface germination method, petri dish germination method, agar plate surface germination method, etc., but these methods all need to obtain a large amount of pu...

Claims

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