Molecular identification method of CGSS (cotton germplasm with stigma showing) gene

A technology of exposed stigma and molecular identification, which is applied in the field of molecular identification of exposed stigma gene identification and molecular marker-assisted breeding, can solve the problems of long-term, low probability, large cotton fields, manpower and financial resources of exposed stigma materials, and achieves accelerated selection. breeding and improvement process, easy operation, high accuracy results

Inactive Publication Date: 2017-01-25
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, in the traditional breeding program relying on phenotypic identification, self-identification is required after each backcross to ensure that the stigma-exposed gene is not lost in the process of transformation and improvement, while the stigma-exposed gene is affected by two pairs of recessive Genetic control, the single plant with exposed stigma has a low probability of appearing in the segregated population, which requires a lot of cotton fields, manpower and financial resources
It takes a long time to select and improve materials with exposed stigmas by conventional breeding techniques

Method used

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  • Molecular identification method of CGSS (cotton germplasm with stigma showing) gene
  • Molecular identification method of CGSS (cotton germplasm with stigma showing) gene

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Embodiment Construction

[0038] The present invention will be further described in detail below in conjunction with the examples.

[0039] Those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be considered as limiting the scope of the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased.

[0040] Tris-Cl: Tris(hydroxymethyl)aminomethane-HCl]; EDTA: Ethylenediaminetetraacetic acid; SDS: Sodium dodecyl sulfate; PVP: Polyethylene Polyvinylpyrrolidone

[0041] All reagents were purchased from: Sangon Bioengineering (Shanghai) Co., Ltd.; purity: analytically pure.

[0042] 1. DNA extraction

[0043] Step 1: Tak...

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Abstract

The invention belongs to the technical field of biology, and relates to a molecular identification method of CGSS (cotton germplasm with stigma showing), particularly gene-specific primer markers for identifying a CGSS-gene-containing cotton strain, wherein the nucleotide sequences are disclosed as SEQ ID NO.1, 2, 3 and 4. The gene-specific markers are utilized to identify the individual plants carrying CGSS gene in the population. The method has the advantages of high identification speed and high accuracy, is simple to operate, and is very suitable for early indoor identification and molecular-marker-assisted selective breeding of CGSS materials.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular identification method for stigma exposed gene identification and molecular marker assisted breeding. Background technique [0002] Exposed stigma refers to a mutant trait of floral organs in cotton. It is manifested that shortly after the budding of the plant (generally 12-20 days), the stigma protrudes and is higher than the corolla, while the stamens are still wrapped in the corolla, and the loose powder is not exposed until the flowering stamens, and the upper half (the base of the stigma) of the stamen anthers often The performance of necrosis leads to difficulty in pollination of stigma, and the boll rate is very low. Due to the characteristic of exposed stigma, this germplasm is also called dioecious line. This mutant is often found in land-sea hybrids of cotton. Previous studies have shown that the trait of exposed stigma is controlled by two pairs o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 张永山陈伟姚金波李燕褚丽
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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